Ọrịa Enterovirus 71 (EV71)
Aha ngwaahịa
Ngwa Nchọpụta Asịd Nucleic HWTS-EV003- Enterovirus 71 (EV71) (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa aka-ụkwụ-ọnụ bụ ọrịa na-efe efe nke enteroviruses (EV) na-akpata. Ugbu a, achọpụtala ụdị serotypes nke enteroviruses 108, nke kewara n'ime otu anọ: A, B, C na D. N'ime ha, enterovirus EV71 na CoxA16 bụ isi nje ndị na-efe efe. Ọrịa a na-emekarị n'ụmụaka na-erubeghị afọ ise, ọ pụkwara ịkpata herpes n'aka, ụkwụ, ọnụ na akụkụ ndị ọzọ. Ọnụ ọgụgụ dị nta nke ụmụaka ga-enwe nsogbu dịka myocarditis, pulmonary edema, na aseptic meningoencephalitis.
Ọwa
| FAM | EV71 |
| ROX | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa itoolu |
| Ụdị ihe atụ | Mmiri oropharyngeal,Mmiri ọgwụ herpes |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500Mpempe/mL |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P Oge-N'ezie LightCycler®Sistemụ PCR 480 n'oge a Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Nhọrọ nke 1.
Ihe na-eme ka a na-ewepụta ihe: Ihe na-eme ka ihe na-ewepụta ihe dị ka Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), a ga-emekwa ihe a na-ewepụta dịka ntuziaka maka ojiji si dị. Ihe ndị a na-ewepụta bụ ihe ndị na-eme ka ihe na-ewepụta ihe dị ka oropharyngeal swabs ma ọ bụ herpes fluid samples sitere n'aka ndị ọrịa a chịkọtara n'ebe ahụ. Tinye ihe ndị a na-anakọtara n'ime ihe na-emepụta ihe dị ka Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) ozugbo, kpụgharịa ha ma gwakọta ha nke ọma, tinye ha n'ọnọdụ okpomọkụ ụlọ maka nkeji ise, wepụ ha wee tụgharịa ha ka ha gwakọta nke ọma, iji nweta RNA nke ihe nlele ọ bụla.
Nhọrọ nke abụọ.
Ihe na-eme ka a na-ewepụta ihe: Ngwa DNA/RNA nke Macro & Micro-Test General (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke enwere ike iji Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., a ga-emekwa mwepụ ahụ dịka ntuziaka maka ojiji si dị. Olu ihe nlele ahụ ewepụtara bụ 200µL, olu mgbapụta akwadoro bụ 80µL.
Nhọrọ nke 3.
Ihe a na-atụ aro maka mwepụta: QIAamp Viral RNA Mini Kit (52904) nke QIAGEN ma ọ bụ TIAnamp Virus DNA/RNA Kit (YDP315-R), a ga-emekwa mwepụta ahụ dịka ntuziaka maka ojiji si dị. Olu ihe nlele ahụ ewepụtara bụ 140μL, olu mwepụta ahụ akwadoro bụ 60µL.
