Enterovirus 71 (EV71)
Aha ngwaahịa
HWTS-EV003-Enterovirus 71 (EV71) Ngwa nchọpụta Nucleic Acid (Fluorescence PCR)
Epidemiology
Ọrịa ọnụ ụkwụ aka bụ ọrịa na-efe efe nke enteroviruses (EV) kpatara.Ugbu a, a chọtara ụdị serotypes nke enteroviruses dị 108, nke kewara ụzọ anọ: A, B, C na D. N'ime ha, enterovirus EV71 na CoxA16 bụ ndị na-akpata ọrịa.Ọrịa a na-emekarị n'ime ụmụaka na-erubeghị afọ 5, ọ pụkwara ịkpata herpes na aka, ụkwụ, ọnụ na akụkụ ndị ọzọ.Ọnụ ọgụgụ dị nta nke ụmụaka ga-enwe nsogbu dị ka myocarditis, pulmonary edema, na aseptic meningoencephalitis.
Ọwa
| FAM | EV71 |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | Oropharyngeal swab,Herpes mmiri |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500 oyiri/ml |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1.
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), na mmịpụta ga-eduzi dị ka ntuziaka maka ojiji.Ihe nlele ndị a amịpụtara bụ swabs oropharyngeal ma ọ bụ ihe nlele mmiri herpes sitere na ndị ọrịa anakọtara na saịtị.Tinye swabs anakọtara n'ime Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) ozugbo, vortex na gwakọta nke ọma, tinye ya na ụlọ okpomọkụ maka nkeji 5, wepụ ha wee tụgharịa ka ọ gwakọta nke ọma, iji nweta RNA. nke ọ bụla sample.
Nhọrọ 2.
Na-atụ aro mmịpụta reagent: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke a pụrụ iji na Macro & Micro-Test). Akpaaka Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., na mmịpụta kwesịrị iduzi dị ka ntuziaka maka ojiji.Olu nlele ewepụtara bụ 200µL, na olu elution akwadoro bụ 80µL.
Nhọrọ3.
Reagenti mmịpụta akwadoro: QIAamp Viral RNA Mini Kit (52904) nke QIAGEN ma ọ bụ TIANAmp Nje Virus DNA/RNA Kit (YDP315-R), na mmịpụta kwesịrị ka eme ya n'ụzọ siri ike dabere na ntuziaka maka ojiji.Olu ihe nlele ewepụtara bụ 140μL, na olu elution akwadoro bụ 60µL.
