Enterovirus Universal
Aha ngwaahịa
HWTS-EV001-Enterovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Ọrịa ọnụ ụkwụ aka bụ ọrịa na-efe efe nke enteroviruses (EV) kpatara.Ugbu a, a chọtara ụdị serotypes nke enteroviruses dị 108, nke kewara ụzọ anọ: A, B, C na D. N'ime ha, enterovirus EV71 na CoxA16 bụ ndị na-akpata ọrịa.Ọrịa a na-emekarị n'ime ụmụaka na-erubeghị afọ 5, ọ pụkwara ịkpata herpes na aka, ụkwụ, ọnụ na akụkụ ndị ọzọ.Ọnụ ọgụgụ dị nta nke ụmụaka ga-enwe nsogbu dị ka myocarditis, pulmonary edema, na aseptic meningoencephalitis.
Ọwa
| FAM | EV RNA |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Oropharyngeal swab,Herpes mmiri |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 oyiri/ml |
| Ngwa ndị ọdabara | Ngwa biosystem 7500/7500 ngwa ngwa PCR ngwa ngwa, QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1.
Ngwa mgbakwunye na-atụ aro: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor ( HWTS-3006B, HWTS-3006C), a ga-ewepụtara ya nke ọma dịka ntuziaka.Olu nlele bụ 200 μL, olu elution akwadoro bụ 80µL.
Nhọrọ 2.
Ngwa mmịpụta akwadoro: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), ekwesịrị ịwepụta ya nke ọma dịka ntuziaka ahụ siri dị.
Nhọrọ3.
Ngwa mmịpụta akwadoro: QIAamp Viral RNA Mini Kit (52904) ma ọ bụ Nucleic Acid Extraction or Purification Kit (YDP315-R), ekwesịrị ịwepụta ya n'ụzọ siri ike dabere na ntuziaka.Olu nlele bụ 140 μL, olu elution akwadoro bụ 60µL.
