Enterovirus zuru ụwa ọnụ
Aha ngwaahịa
Ngwa Nchọpụta Asịd Nucleic zuru ụwa ọnụ nke Enterovirus (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa aka-ụkwụ-ọnụ bụ ọrịa na-efe efe nke enteroviruses (EV) na-akpata. Ugbu a, achọpụtala ụdị serotypes nke enteroviruses 108, nke kewara n'ime otu anọ: A, B, C na D. N'ime ha, enterovirus EV71 na CoxA16 bụ isi nje ndị na-efe efe. Ọrịa a na-emekarị n'ụmụaka na-erubeghị afọ ise, ọ pụkwara ịkpata herpes n'aka, ụkwụ, ọnụ na akụkụ ndị ọzọ. Ọnụ ọgụgụ dị nta nke ụmụaka ga-enwe nsogbu dịka myocarditis, pulmonary edema, na aseptic meningoencephalitis.
Ọwa
| FAM | EV RNA |
| ROX | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | Mmiri oropharyngeal,Mmiri ọgwụ herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500Mpempe/mL |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Ngwa-ngwa Ngwa 7500/7500 nke Ejiri Mee Ihe, QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P Oge-N'ezie LightCycler®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Nhọrọ nke 1.
Ngwa Mwepụta Akwadoro: Ngwa Ngwuputa Macro & Micro-Nnwale General DNA/RNA (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Ngwa Nwepụta Acid Nucleic Automatic Macro & Micro-Nnwale (HWTS-3006B, HWTS-3006C), a ga-ewepụta ya nke ọma dịka ntuziaka si dị. Olu ihe nlele ahụ bụ 200 μL, olu mwepụta akwadoro bụ 80µL.
Nhọrọ nke abụọ.
Ngwa a tụrụ aro maka mwepụta: Ngwa mwepụta ihe nlele Macro & Micro-Test (HWTS-3005-8), a ga-ewepụta ya nke ọma dịka ntuziaka si dị.
Nhọrọ nke 3.
Ngwa a tụrụ aro maka mwepụta: QIAamp Viral RNA Mini Kit (52904) ma ọ bụ Nucleic Acid Extraction or Purification Kit (YDP315-R), a ga-ewepụta ya dịka ntuziaka si dị. Olu ihe nlele ahụ bụ 140 μL, olu mwepụta akwadoro bụ 60µL.
