Ụdị ọrịa iku ume 11 akpọnwụrụ akpọnwụrụ nucleic acid
Aha ngwaahịa
HWTS-RT190 -Freeze-Fiiri-Freeze-Fikiere Ụdị 11 nke Ọrịa iku ume Nucleic Acid Nchọpụta Ngwa (Fluorescence PCR)
Epidemiology
Ọrịa akụkụ iku ume bụ ọrịa dị mkpa na-eyi ahụike mmadụ egwu nke ukwuu. Nnyocha e mere egosiwo na ihe ka ọtụtụ n'ọrịa ndị na-eku ume na-ebute site na nje bacteria na/ma ọ bụ nje na-ebute ọrịa na-ebute ọrịa ahụ, na-eduga n'ịdị njọ nke ọrịa ahụ ma ọ bụ ọbụna ọnwụ. Ya mere, ịchọpụta nje ahụ nwere ike inye ọgwụgwọ ezubere iche ma melite ọnụọgụ ndụ onye ọrịa[1,2]. Agbanyeghị, ụzọ ọdịnala maka ịchọpụta ọrịa na-efe efe na-eku ume gụnyere nyocha nke obere ihe, ọdịbendị nje na nyocha nke immunological. Ụzọ ndị a dị mgbagwoju anya, na-ewe oge, na-achọ nkà na ụzụ, ma nwee mmetụta dị ala. Na mgbakwunye, ha enweghị ike ịchọpụta ọtụtụ nje na-akpata n'otu nlele, na-eme ka o sie ike ịnye ndị dọkịta nyocha ihe enyemaka ziri ezi. N'ihi ya, ọtụtụ ọgwụ ka nọ na ọkwa ọgwụ ike, nke ọ bụghị nanị na-eme ka usoro nkwụsị nke nje na-adị ngwa ngwa, kamakwa na-emetụta nyocha nke ndị ọrịa n'oge[3]. Haemophilus influenzae a na-ahụkarị, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Stenotrophomonas maltophilia, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae pneumoniae. pneumophila bụ nje ndị dị mkpa na-ebute ọrịa na-efe efe nke akụkụ okuku ume na nosocomial [4,5]. Ngwa ule a na-achọpụta ma na-achọpụta ụfọdụ nucleic acid nke nje ndị a dị n'elu n'ime ndị mmadụ nwere akara na akara nke ọrịa iku ume, ma jikọta ya na nsonaazụ ụlọ nyocha ndị ọzọ iji nyere aka na nyocha nke ọrịa nje iku ume.
Nka nka
| Nchekwa | 2-30 ℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Akpịrị swab |
| Ct | ≤33 |
| CV | <5.0% |
| LoD | LoD nke ngwa maka Klebsiella pneumoniae bụ 500 CFU/ml; LoD nke Streptococcus pneumoniae bụ 500 CFU / ml; LoD nke Haemophilus influenzae bụ 1000 CFU/mL; LoD nke Pseudomonas aeruginosa bụ 500 CFU / mL; LoD nke Acinetobacter baumannii bụ 500 CFU/mL; LoD nke Stenotrophomonas maltophilia bụ 1000 CFU / mL; LoD nke Bordetella pertussis bụ 500 CFU / ml; LoD nke Bordetella parapertussis bụ 500 CFU / ml; LoD nke Mycoplasma pneumoniae bụ 200 mbipụta / ml; LoD nke Legionella pneumophila bụ 1000 CFU / mL; LoD nke chlamydia pneumoniae bụ 200 mbipụta / ml. |
| Nkọwapụta | Enweghị mmeghachi omume n'etiti ngwa ahụ na nje ndị ọzọ na-ekesa iku ume na-abụghị ebe a na-achọpụta ngwa ule ahụ, dịka Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Serratia marcescens, Enterococcus faecalis, Candida albicans, Klebsiella oxytoca, Streptonecoccus, Micro-Streptonecoquido, Micro-Staphylococcus, Escherichia. Listeria monocytogenes, Acinetobacter junii, Haemophilus parainfluenzae, Legionella dumov, Enterobacter aerogenes, Haemophilus haemolyticus, Streptococcus salivarius, Streptococcus salivarius, Neisseria meningitidis, Mycobacterium ụkwara nta, Influenza A virus, Influenza A virus, Influenza B nje, Influenza Flav. Aspergillus fumigatus, Candida glabrata, na Candida tropicalis. |
| Ngwa ndị ọdabara | Ụdị M: Ngwa Biosystems 7500 Real-Time PCR Systems, Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa, QuantStudio®Sistemụ PCR 5 ezigbo oge, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR Systems, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, Real-Time PCR Sistemu, PCR-Time PCR System, Real-Time PCR Sistemu, PCR-Time PCR System Ụdị II: EudemonTMAIO800 (HWTS-EQ007) nke Jiangsu Macro & Micro-Test Med-Tech Co. Ltd. |
Usoro ọrụ
Ụdị M: Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) site na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. na-atụ aro ka a na-atụ aro ya na usoro nke FU na-esote. Kit.
