Ụdị ọrịa iku ume iri na otu a kpọrọ nkụ n'ime oyi
Aha ngwaahịa
HWTS-RT190 -Kpọọ nkụ, kpọọ nkụ, kpọọ nkụ. Ụdị ọrịa iku ume iri na otu. Ngwa nchọpụta Acid Nucleic (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa iku ume bụ ọrịa dị mkpa nke na-eyi ahụike mmadụ egwu nke ukwuu. Nnyocha egosila na ọtụtụ ọrịa iku ume na-akpata site na nje bacteria na/ma ọ bụ nje virus ndị na-ebute ọrịa ahụ, na-ebute mmụba nke ọrịa ahụ ma ọ bụ ọbụna ọnwụ. Ya mere, ịchọpụta nje nwere ike inye ọgwụgwọ ezubere iche ma melite ọnụego ndụ onye ọrịa [1,2]. Agbanyeghị, ụzọ ọdịnala maka ịchọpụta nje iku ume gụnyere inyocha obere ihe, ọdịbendị nje bacteria, na nyocha ahụ ike. Usoro ndị a dị mgbagwoju anya, na-ewe oge, na-achọ teknụzụ, ma nwee obere mmetụta. Na mgbakwunye, ha enweghị ike ịchọpụta ọtụtụ nje na otu ihe nlele, na-eme ka ọ sie ike inye ndị dọkịta nchoputa enyemaka ziri ezi. N'ihi ya, ọtụtụ ọgwụ ka nọ n'ọkwa ọgwụ empirical, nke na-abụghị naanị na ọ na-eme ka usoro iguzogide nje dịkwuo ngwa ngwa, kamakwa ọ na-emetụta nchọpụta oge nke ndị ọrịa [3]. Ọrịa Haemophilus influenzae a na-ahụkarị, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Stenotrophomonas maltophilia, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Chlamydia pneumoniae, na Legionella pneumophila bụ nje dị mkpa na-akpata ọrịa iku ume n'ime imi [4,5]. Ngwa nnwale a na-achọpụta ma chọpụta nucleic acids kpọmkwem nke nje ndị a dị n'elu n'ime ndị nwere ihe ịrịba ama na mgbaàmà nke ọrịa iku ume, ma jikọta ya na nsonaazụ ụlọ nyocha ndị ọzọ iji nyere aka na nchọpụta nke ọrịa iku ume.
Usoro Nkà na ụzụ
| Nchekwa | 2-30℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | Swab akpịrị |
| Ct | ≤33 |
| CV | <5.0% |
| LoD | LoD nke ngwa maka Klebsiella pneumoniae bụ 500 CFU/mL; LoD nke Streptococcus pneumoniae bụ 500 CFU/mL; LoD nke Haemophilus influenzae bụ 1000 CFU/mL; LoD nke Pseudomonas aeruginosa bụ 500 CFU/mL; LoD nke Acinetobacter baumannii bụ 500 CFU/mL; LoD nke Stenotrophomonas maltophilia bụ 1000 CFU/mL; LoD nke Bordetella pertussis bụ 500 CFU/mL; LoD nke Bordetella parapertussis bụ 500 CFU/mL; LoD nke Mycoplasma pneumoniae bụ 200 kwa/mL; LoD nke Legionella pneumophila bụ 1000 CFU/mL; Ọnụọgụ nke Chlamydia pneumoniae dị 200 kwa mL. |
| Nkọwapụta | Enweghị mmeghachi omume n'etiti ngwa ahụ na nje ndị ọzọ na-akpata iku ume n'èzí usoro nchọpụta nke ngwa nnwale ahụ, dịka Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Serratia marcescens, Enterococcus faecalis, Candida albicans, Klebsiella oxytoca, Streptococcus pyogenes, Micrococcus luteus, Rhodococcus equi, Listeria monocytogenes, Acinetobacter junii, Haemophilus parainfluenzae, Legionella dumov, Enterobacter aerogenes, Haemophilus haemolyticus, Streptococcus salivaryus, Neisseria meningitidis, Mycobacterium tuberculosis, Influenza A virus, Influenza B virus, Aspergillus flavus, Aspergillus terreus, Aspergillus fumigatus, Candida glabrata, na Candida tropicalis. |
| Ngwa Ndị Dị Mkpa | Ụdị nke Mbụ: Sistemụ Biosystems 7500 Real-Time PCR, Sistemụ Biosystems 7500 Ngwa ngwa Real-Time PCR, QuantStudio®Sistemụ PCR ise n'oge a na-arụ ọrụ, Sistemụ PCR SLAN-96P n'oge a na-arụ ọrụ (Hongshi Medical Technology Co., Ltd.), LightCycler®Sistemụ PCR Oge 480, LineGene 9600 Plus Sistemụ Nchọpụta Oge PCR Oge (FQD-96A, teknụzụ Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR Sistemụ, BioRad CFX Opus 96 Real-Time PCR Sistemụ. Ụdị nke Abụọ: EudemonTMAIO800 (HWTS-EQ007) nke Jiangsu Macro & Micro-Test Med-Tech Co. Ltd. |
Usoro Ọrụ
Ụdị nke Mbụ: Ngwa DNA/RNA nke Macro na Micro-Test Viral (HWTS-3017) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. kwadoro maka mwepụta ihe nlele ahụ, a ga-emekwa usoro ndị na-esote dịka IFU nke Ngwa ahụ siri dị.
