Chlamydia Trachomatis nke a kpọrọ nkụ
Aha ngwaahịa
HWTS-UR032C/D-Ngwa Nchọpụta Acid Nucleic Chlamydia Trachomatis nke akpọnwụrụ akpọnwụ (Enzymatic Probe Isothermal Amplification)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Chlamydia trachomatis (CT) bụ ụdị nje prokaryotic nke na-akpata nje bacteria na mkpụrụ ndụ eukaryotic.[1]A na-ekewa Chlamydia trachomatis n'ime ụdị AK dịka usoro serotype si dị. Ọrịa urogenital tract na-abụkarị site na ụdị trachoma biological variant DK serotypes, a na-egosipụtakarị ụmụ nwoke dị ka urethritis, nke enwere ike iwepụ na-enweghị ọgwụgwọ, mana ọtụtụ n'ime ha na-adịte aka, na-aka njọ mgbe ụfọdụ, enwere ike ijikọ ya na epididymitis, proctitis, wdg.[2]Ndị inyom nwere ike inwe urethritis, cervicitis, wdg, yana nsogbu ndị ka njọ nke salpingitis.[3].
Ọwa
| FAM | Chlamydia trachomatis (CT) |
| ROX | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤30℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | swab nke akpanwa nwanyị Swab nke urethra nwoke Mmamịrị nwoke |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | Mbipụta 400/mL |
| Nkọwapụta | Enweghị mmeghachi omume ọ bụla n'etiti ngwa a na nje ndị ọzọ na-ebute ọrịa genitourinary tract dịka ụdị papillomavirus Human papillomavirus 16, ụdị papillomavirus Human papillomavirus 18, ụdị nje Herpes simplex Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Staphylococcus epidermidis, Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, nje immunodeficiency Human, Lactobacillus casei na DNA genomic human, wdg. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P nke Oge-N'ezie (Hongshi Medical Technology Co., Ltd.) LightCycler®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR BioRad CFX96 n'oge a na sistemụ PCR BioRad CFX Opus 96 n'oge a Sistemụ Nchọpụta Isothermal Fluorescence dị mfe nke oge a na-akpọ Easy Amp(HWTS-1600）. |
Usoro Ọrụ
Nhọrọ nke 1.
Ngwa Mwepụta Ihe Nlereanya Maka Nlereanya Macro & Micro (HWTS-3005-8). A ga-eme mwepụta ahụ dịka IFU siri dị. Tinye DNA ihe nlele ahụ nke ihe nlele ahụ wepụtara n'ime ihe nchekwa mmeghachi omume wee nwalee ya ozugbo na ngwa ahụ, ma ọ bụghị ya, a ga-echekwa ihe nlele ndị ahụ a wepụtara na okpomọkụ 2-8℃ maka ihe karịrị awa 24.
Nhọrọ nke abụọ.
Ngwa DNA/RNA izugbe nke Macro na Micro-Nnwale (HWTS-301)7-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). A ga-eme mwepụta ahụ dịka IFU siri dị, olu mwepụta akwadoro bụ 80μL. A na-ekpo ọkụ DNA sample nke e si n'ụzọ magnetik bead wepụta na 95°C maka nkeji atọ wee saa ya ozugbo na ice maka nkeji abụọ. Tinye DNA sample nke e ji usoro a hazie n'ime ihe nchekwa mmeghachi omume ma nwalee ya na ngwaọrụ ma ọ bụ sample ndị a haziri ahazi kwesịrị ịchekwa n'okpuru -18°C maka ihe karịrị ọnwa anọ. Ọnụọgụ nke ifriizi na ịgbaze ugboro ugboro ekwesịghị ịgafe okirikiri anọ.
