Chlamydia Trachomatis akpọnwụwo akpọnwụrụ
Aha ngwaahịa
HWTS-UR032C/D-Klamydia Trachomatis Nucleic Acid Detection Kit nke akpọnwụ akpọnwụ (Enzymatic Probe Isothermal Amplification)
Epidemiology
Chlamydia trachomatis (CT) bụ ụdị prokaryotic microorganism nke na-akpachapụ anya parasitic na sel eukaryotic.[1].Chlamydia trachomatis na-ekewa n'ime AK serotypes dịka usoro serotype siri dị.Ọrịa urogenital tract na-ebutekarị site na trachoma biological variant DK serotypes, na ụmụ nwoke na-egosipụtakarị dị ka urethritis, nke a na-ewepụ ya na-enweghị ọgwụgwọ, ma ọtụtụ n'ime ha na-aghọ nke na-adịghị ala ala, na-akawanye njọ mgbe ụfọdụ, a pụkwara ijikọta ya na epididymitis, proctitis, wdg.[2].Enwere ike ịkpata ụmụ nwanyị na urethritis, cervicitis, wdg, yana nsogbu ndị ka njọ nke salpingitis.[3].
Ọwa
| FAM | Chlamydia trachomatis (CT) |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤30℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Swab cervical nwanyị Nwoke urethra swab Mmamiri nwoke |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | 400 oyiri/ml |
| Nkọwapụta | Ọ dịghị cross-reactivity n'etiti kit a na ndị ọzọ genitourinary tract ọrịa nje dị ka nnukwu ihe ize ndụ Human papillomavirus ụdị 16, Human papillomavirus ụdị 18, Herpes simplex virus ụdị Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma hominis, Epilloma genital, Mycoplasma Hominis, Epilloma, Epilloma. , Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, Human immunodeficiency virus, Lactobacillus casei na mmadụ genomic DNA, wdg. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler Sistemụ PCR nke BioRad CFX96 na BioRad CFX Opus 96 ezigbo oge PCR Sistemu Sistemụ nchọpụta nke Isothermal dị mfe Amp Real-time Fluorescence(HWTS-1600). |
Usoro ọrụ
Nhọrọ 1.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Ekwesịrị ime mmịpụta ahụ n'ụzọ siri ike dabere na IFU.Tinye ihe nlele DNA nke ihe ntọhapụ sampị ewepụtara n'ime ihe nchekwa mmeghachi omume wee nwalee na ngwa ahụ ozugbo, ma ọ bụ a ga-echekwa ihe ndị amịpụtara na 2-8 ℃ maka ihe karịrị awa 24.
Nhọrọ 2.
Ngwa Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ekwesịrị ime mmịpụta ahụ n'ụzọ siri ike dabere na IFU, na oke elution akwadoro bụ 80μL.Ihe nlele DNA nke usoro bead magnetik ewepụtara na-ekpo ọkụ na 95ºC maka nkeji 3 wee saa ice ozugbo maka nkeji abụọ.Tinye DNA a haziri ahazi n'ime ihe nchekwa mmeghachi omume wee nwalee n'ime ngwa ma ọ bụ na a ga-echekwa ihe ndị a haziri n'okpuru -18 Celsius maka ihe karịrị ọnwa anọ.Ọnụ ọgụgụ nke oyi na-atụ ugboro ugboro na thawing ekwesịghị gafere okirikiri anọ.
