Nje Virus/Influenza B Nje Virus Nucleic Acid akpọnwụwo akpọnwụọ
Aha ngwaahịa
HWTS-RT193-Nje Virus Influenza akpọnwụwo oyi/Nri Influenza B Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Dị ka antigenic dị iche n'etiti NP na M genes, influenza nje nwere ike kewaa anọ ụdị: influenza A virus (IFV A), influenza B virus (IFV B), influenza C virus (IFV C) na influenza D virus (IFV D) . Maka nje influenza A, o nwere ọtụtụ ndị ọbịa na serotypes dị mgbagwoju anya, ma nwee ike gbasaa n'ofe ndị ọbịa site na nchịkọta mkpụrụ ndụ ihe nketa na mgbanwe mgbanwe. Ụmụ mmadụ enweghị ihe nchebe na-adịgide adịgide maka nje influenza A, ya mere ndị mmadụ nọ n'afọ ndụ niile na-enwekarị nsogbu. Ọrịa influenza A bụ nje na-akpata ọrịa influenza kasịnụ. Maka nje influenza B, ọ na-agbasakarị n'obere mpaghara ma enweghị ụdị subtypes ugbu a. Ihe na-ebutekarị ọrịa mmadụ bụ nje influenza nke B/Yamagata ma ọ bụ B/Victoria lineages. N'ime ọrịa influenza a na-ekwenye kwa ọnwa na mba 15 nke mpaghara Eshia-Pacific, ọnụọgụ nyocha nke nje influenza B sitere na 0 ruo 92%. N'adịghị ka nje influenza A, ụfọdụ ndị mmadụ, dị ka ụmụaka na ndị agadi, nwere ike ibute nje influenza B, nke nwere ike ịkpata nsogbu n'ụzọ dị mfe, na-ebute ọbụna ibu karịa ọha mmadụ karịa nje influenza A.
Nka nka
| Nchekwa | 2-28℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Akpịrị swab |
| Ct | IFV A,IFVB Ct≤35 |
| CV | <5.0% |
| LoD | 200 mbipụta/ml |
| Nkọwapụta | Cross-reactivity: Ọ dịghị cross-reactivity n'etiti kit na Bocavirus, Rhinovirus, Cytomegalovirus, Respiratory syncytial virus, Parainfluenza virus, Epstein-Barr virus, herpes simplex virus, varicella-zoster virus, Mumps nje, Enterovirus, Measles nje, mmadụ metapneumovirus, coronavirus Adenovirus, SARS-Covid-19. MERS-CoV, Rotavirus, Norovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumocystis jirovecii, Haemophilus Bordecoccus aurephytus, Staktus, Status, Haemophilus influenza. Mycobacterium ụkwara nta, Neisseria gonorrhoeae, Candida albicans, Candida glabrata, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrhalis, Lactobacillus, Corynebacterium na mmadụ genomic DNA. Nnwale nnyonye anya: Mucin (60mg / ml), ọbara mmadụ (50%), Phenylephrine (2 mg / mL), Oxymetazoline (2mg / mL), sodium chloride (20mg / ml) na 5% preservative, Beclomethasone (20mg / ml), Dexamethasone (20mg/mL), Flulones (2mg/mL), Budesonide (1mg/mL), Mometasone (2mg/mL), Fluticasone (2mg/mL), Histamine hydrochloride (5 mg/mL), Benzocaine (10%), Menthol (10%), Zanamivir (20mg/mL), Peramivir (1mg/mL), Mupirom/Lm. A na-ahọrọ Oseltamivir (60ng / mL), Ribavirin (10mg / L) maka nyocha nnyonye anya, na nsonaazụ ya gosipụtara na ihe ndị na-etinye aka na ihe ndị dị n'elu enweghị mmeghachi omume nnyonye anya na nsonaazụ ule nke ngwa ahụ. |
| Ngwa ndị ọdabara | Ọdabara na ụdị I test reagent: Ngwa Biosystem 7500 ezigbo oge PCR, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.). Ọdabara na ụdị II test reagent: EudemonTM AIO800 (HWTS-EQ007) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Usoro ọrụ
PCR nkịtị
Macro & Micro-Test General DNA / RNA Kit (HWTS-3019) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B)) site na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. na-akwadoro maka nlele anya n'okpuru usoro nke FU.
AIO800 niile-na-otu igwe
