Zaire akpọnwụwo na Sudan Ebola nje Nucleic Acid
Aha ngwaahịa
HWTS-FE035-Freeze-akpọnwụ Zaire na Sudan Ebolavirus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Ebolavirus bụ nke Filoviridae, nke bụ nje RNA na-adịghị mma nke nwere otu eriri. Nje Virus bụ ogologo filaments nwere nkezi virion ogologo nke 1000nm na dayameta nke ihe dịka 100nm. Ọrịa Ebola nje genome bụ eriri RNA na-adịghị mma nke nwere oke 18.9kb, na-edobe protein protein 7 na protein anaghị arụ ọrụ. Enwere ike kewaa oria Ebola n'ụdị dịka Zaire, Sudan, Bundibugyo, Tai Forest na Reston. N'ime ha, ụdị Zaire na ụdị Sudan ka akọọrọ na-ebute ọrịa na-egbu ọtụtụ mmadụ. EHF (Ebola Hemorrhagic Fever) bụ nnukwu ọrịa hemorrhagic na-efe efe nke nje Ebola kpatara. Ndị mmadụ na-ebutekarị ọrịa site na kọntaktị na mmiri ahụ, ihe nzuzo na excreta nke ndị ọrịa ma ọ bụ anụmanụ ndị bu ọrịa, na ngosipụta ụlọ ọgwụ na-ebutekarị ahụ ọkụ, ọbara ọgbụgba na ọtụtụ mmebi akụkụ ahụ. EHF nwere ọnụ ọgụgụ ọnwụ dị elu nke 50%-90%. Ka ọ dị ugbu a, ụzọ a na-esi achọpụta ọrịa Ebola bụ nke kachasị nyocha ụlọ nyocha, gụnyere akụkụ abụọ: nchọpụta etiological na nchọpụta serological. Nchọpụta Etiological gụnyere ịchọpụta antigens viral na nlele ọbara site na ELISA, ịchọpụta acid nucleic site na usoro mmụba dị ka RT-PCR, wdg, na iji mkpụrụ ndụ Vero, Hela, wdg maka ikewapụ nje na omenala. Nchọpụta serological gụnyere ịchọpụta Serum kpọmkwem ọgwụ mgbochi IgM site na ijide ELISA, yana ịchọpụta ọgwụ mgbochi IgG kpọmkwem site na ELISA, immunofluorescence, wdg.
Nka nka
Nchekwa | ≤30℃ |
Ndụ nchekwa | Ọnwa iri na abụọ |
Ụdị ụdị | ọbara, plasma samples |
CV | ≤5.0% |
LoD | 500 oyiri/μL |
Ngwa ndị ọdabara | Ọdabara na ụdị I nchọpụta reagent: Ngwa Biosystem 7500 ezigbo oge PCR, QuantStudio®Sistemụ PCR 5 ezigbo oge, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, teknụzụ Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. Ọdabara maka ụdị II reagent nchọpụta: EudemonTMAIO800 (HWTS-EQ007) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Usoro ọrụ
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ekwesịrị ịme mmịpụta ahụ dị ka ntuziaka ahụ si dị, na nha nlele ewepụtara bụ 200μL na olu elution akwadoro bụ 80μL.