Ụdị nje virus nke Herpes Simplex 1
Aha ngwaahịa
HWTS-UR006 Herpes Simplex Nje Virus Ụdị 1 Ngwa Nchọpụta Nucleic Acid (Fluorescence PCR)
Epidemiology
Ọrịa ndị a na-ebute site ná mmekọahụ (STD) ka bụ otu n'ime ihe ndị dị mkpa na-eyi nchekwa ahụike ọha na eze gburugburu ụwa, nke nwere ike ịkpata amụghị nwa, ịmụ nwa, etuto ahụ na nsogbu dị iche iche dị egwu.[3-6].Enwere ọtụtụ ụdị ọrịa STD, gụnyere nje bacteria, nje, chlamydia, mycoplasma na spirochetes.Ụdị ndị a na-ahụkarị gụnyere neisseria gonorrheae, mycoplasma genitalium, chlamydia trachomatis, herpes simplex virus type 1, herpes simplex virus type 2, mycoplasma hominis, ureaplasma urealyticum, wdg.
Ọwa
FAM | Ụdị nje virus Herpes Simplex 1 (HSV1) |
ROX | Njikwa ime |
Nka nka
Nchekwa | -18 ℃ |
Ndụ nchekwa | Ọnwa iri na abụọ |
Ụdị ụdị | Ọkpụkpụ cervical nwanyị,Nwoke urethra swab |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 500Mpempe akwụkwọ/ml |
Nkọwapụta | Nwalee nje ndị ọzọ na-ebute ọrịa STD, dị ka treponema pallidum, chlamydia trachomatis, neisseria gonorrheae, mycoplasma hominis, mycoplasma genitalium, ureaplasma urealyticum, wdg, ọ dịghị cross-reactivity. |
Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), a ga-eduzi mmịpụta ahụ dịka IFU siri dị.
Nhọrọ 2.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ekwesịrị ịme mmịpụta ahụ dị ka IFU si dị, na oke elution akwadoro bụ 80μL.
Nhọrọ 3.
Nucleic Acid Extraction or Purification Reagent (YDP302) nke Tiangen Biotech (Beijing) Co., Ltd. Ekwesịrị ịme mmịpụta ahụ n'ụzọ siri ike dabere na IFU, na olu elution akwadoro bụ 80μL.
Ekwesịrị ịnwale ụdị DNA ewepụtara ozugbo ma ọ bụ chekwaa ya n'okpuru -18 Celsius maka ihe na-erughị ọnwa 7.Ọnụ ọgụgụ nke oyi na-atụ ugboro ugboro na thawing ekwesịghị gafere okirikiri anọ.