Klebsiella Pneumoniae, Acinetobacter Baumannii na Pseudomonas Aeruginosa na Drug Resistance Genes (KPC, NDM, OXA48 na IMP) Multiplex

Nkọwa dị mkpirikpi:

A na-eji ngwa a maka nchọpụta qualitative in vitro nke Klebsiella pneumoniae (KPN), Acinetobacter baumannii (Aba), Pseudomonas aeruginosa (PA) na mkpụrụ ndụ ihe nketa carbapenem anọ (nke gụnyere KPC, NDM, OXA48 na IMP) na nlele sputum mmadụ, iji nye Ndabere nke nduzi nke nyocha ụlọ ọgwụ, ọgwụgwọ na ọgwụ maka ndị ọrịa na-enyo enyo ọrịa nje.

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HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii na Pseudomonas Aeruginosa na Drug Resistance Genes (KPC, NDM, OXA48 na IMP) Multiplex Detection Kit (Fluorescence PCR)

Asambodo

Epidemiology

Klebsiella pneumoniae bụ ihe na-akpatakarị ohere ụlọ ọgwụ na otu n'ime nje bacteria dị mkpa na-ebute ọrịa nosocomial.Mgbe ahụ ike na-ebelata, nje bacteria na-abanye na ngụgụ site na iku ume, na-ebute ọrịa n'ọtụtụ akụkụ ahụ, na iji ọgwụ nje mee ihe n'oge bụ isi ihe na-agwọ ọrịa.^[1].

Ebe a na-ahụkarị ọrịa Acinetobacter baumannii bụ ngụgụ, nke bụ ihe dị mkpa na-akpata ọrịa oyi baa n'ụlọ ọgwụ (HAP), karịsịa Ventilator jikọtara oyi baa (VAP).Ọ na-esonyere ya na ọrịa nje ndị ọzọ na-efe efe na fungal, nwere njirimara nke ọnụọgụ ọrịa dị elu na ọnụ ọgụgụ ọnwụ dị elu.

Pseudomonas aeruginosa bụ ihe na-adịghị fermentative gram-negative bacilli na-emekarị na ụlọ ọgwụ, na ọ bụ ihe dị mkpa opportunistic pathogen maka ọrịa na-enweta ụlọ ọgwụ, na e ji mara mfe colonization, mfe mgbanwe na multi-ọgwụ mgbochi.

Ọwa

Aha	PCR-Mix 1	PCR-Mix 2
Ọwa FAM	Aba	IMP
Ọwa VIC/HEX	Njikwa ime	KPC
Ọwa CY5	PA	NDM
Ọwa ROX	KPN	OXA48

Nka nka

Nchekwa	≤-18℃
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ụdị	Sputum
Ct	≤36
CV	≤10.0%
LoD	1000 CFU/ml
Nkọwapụta	A) Nnwale mmeghachi omume nke cross-reactivity na-egosi na ngwa a enweghị ihe ọ bụla na-emeghachi omume na nje ndị ọzọ na-eku ume iku ume, dị ka Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelii, Acinetobacter, hemolytic acid, pepeumonia. fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus na sputum samples na-enweghị ebumnuche, wdg. b) Ikike mgbochi nnyonye anya: Họrọ mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid na roxithromycin, wdg. etinyela aka na nchọpụta Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa na carbapenem eguzogide mkpụrụ ndụ ihe nketa KPC, NDM, OXA48 na IMP.
Ngwa ndị ọdabara	Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio^®Sistemụ PCR 5 ezigbo oge LightCycler^®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge