HPV 16 na HPV 18
Aha ngwaahịa
HWTS-CC001-Ngwa Nchọpụta Asịd Nucleic nke HPV16 na HPV18 (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa kansa olu bụ otu n'ime etuto ọjọọ kachasị na-eme n'ime ụmụ nwanyị. Egosila na ọrịa HPV na-adịgide adịgide na ọtụtụ ọrịa bụ otu n'ime ihe ndị bụ isi na-akpata ọrịa kansa olu. Ugbu a, enwere enweghị ọgwụgwọ dị irè a na-anabatakarị maka ọrịa kansa olu nke HPV na-akpata. Ya mere, nchọpụta mbụ na mgbochi nke ọrịa olu nke HPV na-akpata bụ isi ihe na-egbochi ọrịa kansa olu. Ịmepụta ule nchọpụta dị mfe, kpọmkwem na ngwa ngwa maka nje dị oke mkpa maka nchọpụta ọrịa kansa olu.
Ọwa
| Ọwa | Ụdị |
| FAM | HPV18 |
| VIC/HEX | HPV16 |
| CY5 | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | mkpụrụ ndụ a na-ewepụ n'ime cervical |
| Ct | ≤28 |
| CV | ≤10.0% |
| LoD | Mbipụta 500/mL |
| Nkọwapụta | Mgbe ejiri ngwa ahụ nwalee ihe nlele ndị na-abụghị nke a kapịrị ọnụ nke nwere ike imeghachi omume na ihe mgbaru ọsọ ya, nsonaazụ niile na-adịghị mma, gụnyere Ureaplasma urealyticum, Chlamydia trachomatis, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mildew, Gardnerella na ụdị HPV ndị ọzọ nke ngwa ahụ na-ekpuchighị. |
| Ngwa Ndị Dị Mkpa | SLAN®Sistemụ PCR oge-oge -96PSistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ LightCycler®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Ngwa Okpomọkụ Ọnụọgụ Oge A. |
Usoro Ọrụ
Nhọrọ nke 1.
Ngwa Mwepụta Ihe Nlereanya Macro & Micro-Test (HWTS-3005-8), a ga-ewepụta ya dịka ntuziaka si dị.
Nhọrọ nke abụọ.
Ngwa Nnwale Ngwa DNA/RNA nke Ngwa Macro na Micro (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, HWTS-3006C), a ga-ewepụta ya dịka ntuziaka si dị. Olu ihe nlele ewepụtara bụ 200μL, olu mmiri akwadoro bụ 80µL.
Nhọrọ nke 3.
Ngwa QIAamp DNA Mini Kit (51304) nke QIAGEN ma ọ bụ TIAnamp Virus DNA/RNA Kit (YDP315) nke Tiangen Biotech (Beijing) Co., Ltd. mepụtara, a ga-ewepụta ya dịka ntuziaka si dị. Olu ihe nlele ewepụtara bụ 200μL, olu mmiri akwadoro bụ 80µL.
