HPV16 na HPV18
Aha ngwaahịa
HWTS-CC001-Ngwa HPV16 na HPV18 Nchọpụta Nucleic Acid (Fluorescence PCR)
Epidemiology
Ọrịa kansa cervical bụ otu n'ime etuto ọjọọ na-adịkarị na traktị ọmụmụ ụmụ nwanyị.E gosiputara na ọrịa HPV na-adịgide adịgide na ọtụtụ ọrịa na-efe efe bụ otu n'ime isi ihe na-ebute ọrịa kansa cervical.Ka ọ dị ugbu a, a ka enwekwaghị ọgwụgwọ dị mma nke a na-anabatakarị maka ọrịa kansa cervical nke HPV kpatara.Ya mere, nchọpụta mmalite na mgbochi ọrịa cervical nke HPV kpatara bụ igodo iji gbochie ọrịa cancer nke cervical.Ịmepụta ule nyocha dị mfe, nke akọwapụtara na ngwa ngwa maka ọrịa nje dị oke mkpa maka nyocha ụlọ ọgwụ nke ọrịa cancer cervical.
Ọwa
| Ọwa | Ụdị |
| FAM | HPV18 |
| VIC/HEX | HPV16 |
| CY5 | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | cell exfoliated cervical |
| Ct | ≤28 |
| CV | ≤10.0% |
| LoD | 500 oyiri/ml |
| Nkọwapụta | Mgbe ị na-eji ngwa ahụ nwalee ụdị ndị na-abụghị nke nwere ike ịgafe na ebumnuche ya, nsonaazụ niile adịghị mma, gụnyere Ureaplasma urealyticum, Chlamydia trachomatis, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mildew, Gardnerella na-adịghị ekpuchi na ụdị HPV ndị ọzọ. ngwa ahụ. |
| Ngwa ndị ọdabara | SLAN®-96P Real-Time PCR SystemsNgwa Biosystem 7500 ezigbo oge PCR Sistemu QuantStudio®Sistemụ PCR 5 ezigbo oge LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler. |
Usoro ọrụ
Nhọrọ1.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), ekwesịrị ịwepụta ya dịka ntuziaka ahụ si dị.
Nhọrọ2.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, HWTS-3006C), a ga-ewepụta ya dị ka ntuziaka.Olu ihe nlele ewepụtara bụ 200μL, na olu elution akwadoro bụ 80µL.
Nhọrọ3.
Ngwa QIAamp DNA Mini (51304) nke QIAGEN ma ọ bụ TIANAmp Virus DNA/RNA Kit rụpụtara (YDP315) nke Tiangen Biotech (Beijing) Co., Ltd rụpụtara, ekwesịrị iwepụta ya n'ụzọ siri ike dabere na ntuziaka ahụ.Olu ihe nlele ewepụtara bụ 200μL, na olu elution akwadoro bụ 80µL.
