Mmadu BRAF Gene V600E
Aha ngwaahịa
HWTS-TM007-Ihe nchọta mgbanwe mmadụ BRAF Gene V600E(Fluorescence PCR)
Asambodo
CE/TFDA
Epidemiology
Achọpụtala ihe karịrị ụdị mmụgharị BRAF 30, nke ihe dịka 90% dị na exon 15, ebe a na-ewere mmụgharị V600E dị ka mmụgharị a na-ahụkarị, ya bụ, thymine (T) na ọnọdụ 1799 na exon 15 ka agbanwere ka ọ bụrụ mmụgharị. adenine (A), na-ebute nnọchi nke valine (V) na ọnọdụ 600 site na glutamic acid (E) na ngwaahịa protein.A na-ahụkarị mmụgharị BRAF na etuto ọjọọ dị ka melanoma, cancer colorectal, cancer thyroid, na kansa akpa ume.Ịghọta ngbanwe nke mkpụrụ ndụ ihe nketa BRAF aghọwo ihe dị mkpa iji nyochaa EGFR-TKIs na BRAF gene mutation ọgwụ na ọgwụgwọ ọgwụ ezubere iche maka ndị ọrịa nwere ike irite uru.
Ọwa
| FAM | Mgbanwe V600E, njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | paraffin-agbakwunyere anụ ahụ anụ ahụ |
| CV | 5.0% |
| Ct | ≤38 |
| LoD | Jiri ngwa ahụ chọpụta njikwa mma LoD kwekọrọ.a) n'okpuru ụdị ụdị anụ ọhịa 3ng / μL, enwere ike ịhụ ọnụego mmụgharị 1% na nchekwa mmeghachi omume kwụsiri ike;b) n'okpuru 1% mmụgharị, ngbanwe nke 1 × 103Mpempe akwụkwọ/ml dị n'azụ ụdị ọhịa nke 1 × 105Enwere ike ịhụ nnomi/mL nke ọma na nchekwa mmeghachi omume;c) IC Reaction Buffer nwere ike ịchọpụta njikwa njikwa kacha ala SW3 nke njikwa ime ụlọ ọrụ. |
| Ngwa ngwa: | Ngwa Biosystem 7500 ezigbo oge PCR SistemuNgwa Biosystem 7300 ezigbo oge PCR Sistemu, QuantStudio® 5 Sistemụ PCR ezigbo oge Sistemụ PCR Real-Time LightCycler®480 BioRad CFX96 ezigbo oge PCR sistemụ |
Usoro ọrụ
Ihe nrụpụta ihe akwadoro: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Ngwa DNA Rapid Extraction Kit (DP330) nke Tiangen Biotech(Beijing) Co., Ltd rụpụtara.
