PML-RARA Fusion Gene Mutation mmadụ
Aha ngwaahịa
HWTS-TM017ANgwa PML-RARA Fusion Gene Mutation Detection Kit (Fluorescence PCR)
Epidemiology
Nnukwu ọrịa leukemia promyelocytic (APL) bụ ụdị pụrụ iche nke nnukwu ọrịa leukemia myeloid (AML). Ihe dị ka 95% nke ndị ọrịa APL na-esonyere mgbanwe cytogenetic pụrụ iche, ya bụ t (15;17) (q22;q21), nke na-eme ka mkpụrụ ndụ PML na chromosome 15 na retinoic acid α gene (RARA) na chromosome 17 fused iji mepụta PML-RARA fusion gene. N'ihi nkwụsịtụ dị iche iche nke mkpụrụ ndụ PML, enwere ike kewaa PML-RARA fusion gene n'ụdị ogologo (ụdị L), ụdị mkpụmkpụ (ụdị S) na ụdị dị iche iche (ụdị V), na-aza ihe dịka 55%, 40% na 5% n'otu n'otu.
Ọwa
| FAM | Mkpụrụ ndụ ihe nketa PML-RARA |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ N'ọchịchịrị |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | ụmị ọkpụkpụ |
| CV | <5.0 nke |
| LoD | 1000 oyiri/ml. |
| Nkọwapụta | Enweghị mmeghachi omume na-emeghachi omume na mkpụrụ ndụ ihe nketa ndị ọzọ BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, na TEL-AML1 mkpụrụ ndụ ihe nketa. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Reagenti mmịpụta akwadoro: RNAprep Ọbara Ngụkọta Mgbapụta RNA (DP433). Ekwesịrị ịme mmịpụta ahụ dịka IFU si kwuo.
