Ụdị nje 4 na-eku ume
Aha ngwaahịa
HWTS-RT099-4 ụdịdị nke nje virus iku ume ngwa ngwa nchọpụta Acid (Fluorescence PCR)
Epidemiology
Ọrịa Corona Virus 2019, nke akpọrọ "COVID-19", na-ezo aka na oyi baa na-akpata.2019-nCoVọrịa.2019-nCoVbụ coronavirus bụ nke β genus.COVID-19 bụ ọrịa na-efe efe na-efe efe na-efe efe na-efe efe, ndị mmadụ na-enwekarịkwa ike.Ka ọ dị ugbu a, isi mmalite nke ọrịa bụ ndị ọrịa butere ya2019-nCoV, na ndị butere ọrịa asymptomatic nwekwara ike bụrụ isi mmalite nke ọrịa.Dabere na nyocha ọrịa ọrịa ugbu a, oge nnabata bụ ụbọchị 1-14, ọkachasị ụbọchị 3-7.Ahụ ọkụ, ụkwara akọrọ na ike ọgwụgwụ bụ isi ihe ngosi.Ndị ọrịa ole na ole nwere akaras dị kaimi imi, imi imi, akpịrị akpịrị, myalgia na afọ ọsịsa, wdg.
Ọwa
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Njikwa ime |
Nka nka
| Nchekwa | -18 ℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300 oyiri/mlInfluenza A virus/Influenza B nje/Nje virus syncytial iku ume: 500Copy/mL |
| Nkọwapụta | a) Nsonaazụ mmeghachi omume na-egosi na enweghị mmeghachi omume n'etiti ngwa ahụ na coronavirus mmadụ SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, ụdị nje parainfluenza 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus mmadụ, enterovirus A, B, C, D, nje pulmonary mmadụ, nje epstein-barr, nje measles, nje cytomegalo mmadụ, rotavirus, norovirus, nje parotitis, varicella-zoster. nje, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, klebsiella pneumoniae, mycobacterium ụkwara nta, anwụrụ ọkụ aspergillus, candida albicans, candidanemic pneumonia na-amụ ọhụrụ acid na kriptococcus. . b) Ikike mgbochi nnyonye anya: họrọ mucin (60mg / mL), 10% (v / v) nke ọbara na phenylephrine (2mg / mL), oxymetazoline (2mg / mL), sodium chloride (gụnyere preservatives) (20 mg / ml). Beclomethasone (20mg / ml), dexamethasone (20mg / ml), flunisolide (20μg / ml), triamcinolone acetonide (2mg / ml), budesonide (2mg / ml), mometasone (2mg / ml), fluticasone (2mg / ml). ), histamine hydrochloride (5mg/mL), alpha interferon (800IU/ml), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/ Ritonavir (60 mg / ml), mupirocin (20mg / ml), azithromycin (1mg / ml), ceftriaxone (40μg / ml), meropenem (200mg / ml), levofloxacin (10μg / ml) na tobramycin (0.6mg/mL). mL) maka nyocha nnyonye anya, nsonaazụ ya na-egosi na ihe ndị na-etinye aka na mkpokọta ndị a kpọtụrụ aha n'elu enweghị mmeghachi omume nnyonye anya na nsonaazụ nnwale nke nje. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR ezigbo oge QuantStudio5 |
Usoro ọrụ
Nhọrọ 1.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) nke Jiangsu Macro & Micro mepụtara. -Test Med-Tech Co., Ltd. The amịpụtara sample olu bụ 200μL, na akwadoro elution olu bụ 80μL.
Nhọrọ 2.
QIAamp Viral RNA Mini Kit (52904) mepụtara site na QIAGEN ma ọ bụ Nucleic Acid Extraction or Purification Kit (YDP315-R) nke Tiangen Biotech (Beijing) Co., Ltd mepụtara. Ihe nlele amịpụtara bụ 140μL, na olu elution akwadoro bụ 60μL.
