Influenza A/B
Aha ngwaahịa
Ngwa HWTS-RT003A Influenza A/B Nchọpụta Nucleic Acid (Fluorescence PCR)
Epidemiology
Influenza A bụ nnukwu ọrịa na-efe efe nke iku ume, nwere ọtụtụ ụdị dị ka H1N1 na H3N2, ndị na-enwekarị mmụpụta ma gbasaa n'ụwa niile.Mgbanwe Antigenic na-ezo aka na mmụgharị nke nje influenza A, na-ebute mpụta nke subtype ọhụrụ.A na-ekewa nje virus influenza B ụzọ abụọ bụ isi, Yamagata na Victoria.Nje virus influenza B nwere naanị antigenic ịkpafu, na ha na-agbanarị onyunyo na mkpochapụ nke usoro ahụ ji alụso ọrịa ọgụ mmadụ site na ngbanwe ha.Otú ọ dị, evolushọn ọnụ ọgụgụ evolushọn nke influenza B nje ji nwayọọ karịa nke influenza A nje, na influenza B nje nwekwara ike ịkpata mmadụ traktị iku ume ọrịa na-eduga na-efe efe.
Ọwa
| FAM | IFV A |
| ROX | Njikwa ime |
| VIC/HEX | IFV B |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | oropharyngeal swab |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | IFV A: 500 oyiri / ml, IFV B: 500 oyiri / ml |
| Nkọwapụta | 1. Cross-reactivity: ọ dịghị cross reactivity n'etiti a kit na adenovirus ụdị 3, 7, mmadụ coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, na HCoV-NL63, cytomegalovirus, enterovirus, nje parainfluenza, nje measles, metapneumovirus mmadụ, nje mumps, nje syncytial nke akụkụ okuku ume na ụdị B, rhinovirus, bordetella pertussis, chlamydia pneumoniae, corynebacterium, escherichia coli, haemophilus influenzae, jactobacularlenthabacteria, pneumonia, pneumonia, pneumonia. e, neisseria meningitidis , neisseria gonorrhoeae, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, streptococcus pyogenes, streptococcus salivarius na mmadụ genomic DNA. 2. Nnwale nnyonye anya: A na-ahọrọ ihe ndị na-etinye aka dị ka mucin (60mg / mL), ọbara mmadụ, oxymetazoline (2mg / mL), sọlfọ (10%), beclomethasone (20mg / mL), dexamethasone (20mg / mL), flunisolide ( 20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL). ), azithromycin (1mg/L), cephalosporin (40μg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir phosphate (60ng/mL), ribavirin (10mg/L), na nsonaazụ gosiri. na ihe ndị na-etinye aka na ntinye uche dị n'elu enweghị mmeghachi omume na-egbochi nchoputa nke ngwa ahụ. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistemụ PCR nke BioRad CFX96 na BioRad CFX Opus 96 ezigbo oge PCR Sistemu |
Usoro ọrụ
Nhọrọ 1.
Ndị na-emegharị mmịpụta akwadoro: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke enwere ike iji Macro & Micro-Test). Akpaaka Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekwesịrị ịme mmịpụta dịka IFU si dị.The mmịpụta sample olu bụ200μL.Olu elution akwadoro bụ 80μL.
Nhọrọ 2.
Reagents mmịpụta akwadoro: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Ekwesịrị ịme mmịpụta ahụ dịka IFU si kwuo.
Nhọrọ 3.
Reagents mmịpụta akwadoro: Mwepụta Nucleic Acid ma ọ bụ Ngwa Ọcha (YDP315-R).Ekwesịrị ịme mmịpụta ahụ dịka IFU si kwuo.Olu nke mmịpụta bụ 140 μl.Oke elution akwadoro bụ 60μL.
