Klebsiella Pneumoniae, Acinetobacter Baumannii na Pseudomonas Aeruginosa na Drug Resistance Genes (KPC, NDM, OXA48 na IMP) Multiplex

Nkọwa dị mkpirikpi:

Nke a kit na-eji maka in vitro qualitative nchọpụta nke Klebsiella pneumoniae (KPN), Acinetobacter baumannii (Aba), Pseudomonas aeruginosa (PA) na anọ carbapenem eguzogide mkpụrụ ndụ ihe nketa (nke na-agụnye KPC, NDM, OXA48 na IMP) na mmadụ sputum ọrịa ndabere na-enyo enyo na ọgwụgwọ nke ọgwụ na-enye ndị na-agwọ ọrịa.

Zitere anyị email

Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii na Pseudomonas Aeruginosa na Drug Resistance Genes (KPC, NDM, OXA48 na IMP) Multiplex Detection Kit (Fluorescence PCR)

Asambodo

Epidemiology

Klebsiella pneumoniae bụ ihe na-akpatakarị ohere ụlọ ọgwụ na otu n'ime nje bacteria dị mkpa na-ebute ọrịa nosocomial. Mgbe ahụ ike na-ebelata, nje bacteria na-abanye na ngụgụ site na iku ume, na-ebute ọrịa n'ọtụtụ akụkụ ahụ, na iji ọgwụ nje mee ihe n'oge bụ isi ihe na-agwọ ọrịa.^[1].

Ebe a na-ahụkarị ọrịa Acinetobacter baumannii bụ ngụgụ, nke bụ ihe dị mkpa na-akpata ọrịa oyi baa n'ụlọ ọgwụ (HAP), karịsịa Ventilator jikọtara oyi baa (VAP). Ọ na-esonyere ya na ọrịa nje ndị ọzọ na-efe efe na fungal, nwere njirimara nke oke ọrịa na ọnụ ọgụgụ ọnwụ dị elu.

Pseudomonas aeruginosa bụ bacilli na-adịghị fermentative gram-negative na omume ụlọ ọgwụ, ọ bụkwa ihe dị mkpa na-enye ohere maka ọrịa na-ebute ụlọ ọgwụ, nke nwere njirimara nke nchịkwa dị mfe, mgbanwe dị mfe na nkwụsị nke ọtụtụ ọgwụ.

Ọwa

Aha	PCR-Mix 1	PCR-Mix 2
Ọwa FAM	Aba	IMP
Ọwa VIC/HEX	Njikwa ime	KPC
Ọwa CY5	PA	NDM
Ọwa ROX	KPN	OXA48

Nka nka

Nchekwa	≤-18℃
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ụdị	Sputum
Ct	≤36
CV	≤10.0%
LoD	1000 CFU/ml
Nkọwapụta	A) Nnwale mmeghachi omume nke cross-reactivity na-egosi na ngwa a enweghị ihe ọ bụla na-emeghachi omume na nje ndị ọzọ na-eku ume iku ume, dị ka Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelii, Acinetobacter, hemophilatic hemolytic. Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus na sputum samples na-enweghị ebumnuche, wdg. b) Ikike mgbochi nnyonye anya: Họrọ mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid na roxithromycin, wdg maka nnwale nnyonye anya, na nsonaazụ ya na-egosi na nnyonye anya ihe ndị a kpọtụrụ aha n'elu adịghị egbochi nnyonye anya. Acinetobacter baumannii, Pseudomonas aeruginosa na carbapenem eguzogide mkpụrụ ndụ ihe nketa KPC, NDM, OXA48 na IMP.
Ngwa ndị ọdabara	Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio^®Sistemụ PCR 5 ezigbo oge LightCycler^®480 ezigbo oge PCR usoro Sistemụ nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge