Klebsiella Pneumoniae, Acinetobacter Baumannii na Pseudomonas Aeruginosa na Mkpụrụ ndụ ihe nketa na-eguzogide ọgwụ (KPC, NDM, OXA48 na IMP) Multiplex
Aha ngwaahịa
HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii na Pseudomonas Aeruginosa na Mkpụrụ ndụ ihe nketa na-eguzogide ọgwụ (KPC, NDM, OXA48 na IMP) Ngwa Nchọpụta Multiplex (Fluorescence PCR)
Asambodo
CE
Ọmụmụ ihe gbasara ọrịa na-efe efe
Klebsiella pneumoniae bụ nje bacteria a na-ahụkarị n'ụlọọgwụ, ọ bụkwa otu n'ime nje bacteria dị mkpa na-akpata ọrịa n'oge na-adịghị anya. Mgbe ahụ mmadụ na-ebelata ike iguzogide ya, nje bacteria ahụ na-abanye n'akpa ume site na akụkụ iku ume, na-ebute ọrịa n'ọtụtụ akụkụ ahụ, iji ọgwụ nje eme ihe n'oge bụ isi ihe dị mkpa iji gwọọ ya.[1].
Ebe a na-ahụkarị ọrịa Acinetobacter baumannii bụ ngụgụ, nke bụ ihe dị mkpa na-akpata ọrịa pneumonia nke ụlọ ọgwụ (HAP), ọkachasị nke metụtara Ventilator (VAP). Ọ na-abụkarị ọrịa nje bacteria na fungal ndị ọzọ na-eso ya, yana njirimara nke oke ọrịa na oke ọnwụ.
Pseudomonas aeruginosa bụ nje bacteria kachasị na-anaghị agbaze agbaze na gram-negative n'ọgwụgwọ, ọ bụkwa nje bacteria dị mkpa maka ọrịa ndị a na-ebute n'ụlọọgwụ, nwere njirimara nke ịdị mfe nke ịmịnye mmadụ n'ime ụlọ, mgbanwe dị mfe na iguzogide ọtụtụ ọgwụ.
Ọwa
| Aha | Ngwakọta PCR-1 | PCR-Mix 2 |
| Ọwa FAM | Aba | IMP |
| Ọwa VIC/HEX | Njikwa Dị n'ime | KPC |
| Ọwa CY5 | PA | NDM |
| Ọwa ROX | KPN | OXA48 |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | Sputum |
| Ct | ≤36 |
| CV | ≤10.0% |
| LoD | 1000 CFU/mL |
| Nkọwapụta | a) Nnwale mmeghachi omume nke cross-reactivity na-egosi na ngwa a enweghị mmeghachi omume cross-reactivity na nje ndị ọzọ na-efe efe, dịka Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus na sputum samples na-enweghị ihe mgbaru ọsọ, wdg. b) Ike mgbochi nnyonye anya: Họrọ mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, na roxithromycin, wdg. maka ule nnyonye anya, nsonaazụ ya na-egosi na ihe nnyonye anya ndị a kpọtụrụ aha n'elu anaghị egbochi nchọpụta nke Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa na mkpụrụ ndụ ihe nketa carbapenem KPC, NDM, OXA48 na IMP. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ LightCycler®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị (FQD-96A, teknụzụ Bioer) MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya (Suzhou Molarray Co., Ltd.) Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Ngwọta PCR zuru oke
