Klebsiella Pneumoniae, Acinetobacter Baumannii na Pseudomonas Aeruginosa na Drug Resistance Genes (KPC, NDM, OXA48 na IMP) Multiplex
Aha ngwaahịa
HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii na Pseudomonas Aeruginosa na Drug Resistance Genes (KPC, NDM, OXA48 na IMP) Multiplex Detection Kit (Fluorescence PCR)
Asambodo
CE
Epidemiology
Klebsiella pneumoniae bụ ihe na-akpatakarị ohere ụlọ ọgwụ na otu n'ime nje bacteria dị mkpa na-ebute ọrịa nosocomial. Mgbe mgbochi ahụ na-ebelata, nje bacteria na-abanye na ngụgụ site na iku ume, na-ebute ọrịa n'ọtụtụ akụkụ nke ahụ, na iji ọgwụ nje mee ihe n'oge bụ isi ihe na-agwọta [1] .Ebe a na-ahụkarị ọrịa Acinetobacter baumannii bụ ngụgụ, nke bụ ihe dị mkpa pathogen maka Ụlọ Ọgwụ nwetara oyi baa (HAP), karịsịa Ventilator metụtara oyi oyi (VAP). A na-ejikọta ya na nje ndị ọzọ na-efe efe na ọrịa fungal, na njirimara nke ọnụ ọgụgụ ọrịa dị elu na ọnụ ọgụgụ dị elu nke ọnwụ.
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Sputum |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 oyiri/ml |
| Nkọwapụta | A) Nnwale mmeghachi omume nke cross-reactivity na-egosi na ngwa a enweghị ihe ọ bụla na-emeghachi omume na nje ndị ọzọ na-eku ume iku ume, dị ka Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelii, Acinetobacter, hemophilatic hemolytic. Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus na sputum samples na-enweghị ebumnuche, wdg. b) Ikike mgbochi nnyonye anya: Họrọ mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid na roxithromycin, wdg maka nnwale nnyonye anya, na nsonaazụ ya na-egosi na nnyonye anya ihe ndị a kpọtụrụ aha n'elu adịghị egbochi nnyonye anya. Acinetobacter baumannii, Pseudomonas aeruginosa na carbapenem eguzogide mkpụrụ ndụ ihe nketa KPC, NDM, OXA48 na IMP. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR, Ngwa biosystem 7500 ngwa ngwa PCR ngwa ngwa, QuantStudio®Sistemụ PCR 5 ezigbo oge, LightCycler®480 ezigbo oge PCR usoro, Sistemụ nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Sistemụ PCR nke BioRad CFX96, BioRad CFX Opus 96 PCR ezigbo oge. |
Usoro ọrụ
Macro & Micro-Test General DNA / RNA Kit (HWTS-3019) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B)) site na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. na-akwadoro maka nlele anya n'okpuru usoro nke FU.
