Ihe Nlereanya Mwepụta Ihe Nlereanya
Aha ngwaahịa
Ihe nnọchite anya mwepụta ihe nlele Macro na Micro-Ule
Asambodo
CE, FDA, NMPA
Isi ihe mejupụtara ya
| Aha | Isi ihe mejupụtara ya | Akụkụnkọwapụta | Ọnụọgụ |
| Mwepụta ihe atụihe mmeghachi omume | Dithiothreitol, sodium dodecylsulfate (SDS), ihe mgbochi RNase,mmiri surfactant, mmiri dị ọcha | 0.5mL/Valụ | Vial iri ise |
Rịba ama: A naghị agbanwe ihe ndị dị na ngwa dị iche iche.
Ọnọdụ nchekwa na oge nchekwa
Debe ma bufee ya n'ọnọdụ okpomọkụ dị n'ime ụlọ. Ndụ nchekwa ya bụ ọnwa iri abụọ na anọ.
Ngwaọrụ ndị dị mkpa
Ngwa na akụrụngwa n'oge nhazi ihe nlele, dịka pipettes, vortex mixers,ịsa mmiri, wdg.
Ihe nlele achọrọ
Swab oropharyngeal a chịkọtara ọhụrụ, swab nasopharyngeal.
Nkọcha nkenke
Mgbe ejiri ngwa a wepụta CV ntụaka ziri ezi n'ime ụlọ maka ihe iri e ji mee ihe, ọnụọgụ mgbanwe (CV, %) nke uru Ct agaghị agafe 10%.
Ọdịiche dị n'etiti ogbe
Mgbe a nwalere ntụaka ziri ezi n'ime ụlọ na ngwugwu atọ nke ngwa nnwale mgbe ewepụtara ya ugboro ugboro, ọnụọgụ mgbanwe (CV, %) nke uru Ct adịghị karịa 10%.
Ntụnyere arụmọrụ
● Nhazi arụmọrụ mwepụta
| Ntụnyere arụmọrụ nke usoro ihe ndọta magnetik na ihe mwepụta ihe atụ | ||||
| ntinye uche | usoro ihe ndọta magnetik | ihe mwepụta ihe nlele | ||
| orfab | N | orfab | N | |
| 20000 | 28.01 | 28.76 | 28.6 | 29.15 |
| 2000 | 31.53 | 31.9 | 32.35 | 32.37 |
| 500 | 33.8 | 34 | 35.25 | 35.9 |
| 200 | 35.25 | 35.9 | 35.83 | 35.96 |
| 100 | 36.99 | 37.7 | 38.13 | emebeghị |
Arụmọrụ mwepụta nke ihe nlele ahụ yiri nke usoro magnetik beads, na ntinye nke nje nwere ike ịbụ 200Copies/mL.
● Ntụnyere uru CV
| Imeghachi nke mwepụta ihe nlele ihe nlele | ||
| ntinye uche:5000Ndepụta/mL | ORF1ab | N |
| 30.17 | 30.38 | |
| 30.09 | 30.36 | |
| 30.36 | 30.26 | |
| 30.03 | 30.48 | |
| 30.14 | 30.45 | |
| 30.31 | 30.16 | |
| 30.38 | 30.7 | |
| 30.72 | 30.79 | |
| CV | 0.73% | 0.69% |
Mgbe a nwalere ya na 5,000 copies /mL, CV nke orFab na N bụ 0.73% na 0.69%, n'otu n'otu.
