Mycobacterium ụkwara nta INH na-eguzogide
Aha ngwaahịa
HWTS-RT002A-Mycobacterium ụkwara nta nke Isoniazid Nchọpụta Nguzogide (Fluorescence PCR)
Asambodo
Myanmar FDA
Epidemiology
Isoniazid, bụ isi ọgwụ mgbochi ụkwara nta ewepụtara na 1952, bụ otu n'ime ọgwụ kachasị dị irè maka ọgwụgwọ ụkwara nta na-arụ ọrụ na otu ọgwụ maka ụkwara nta.
KatG bụ isi mkpụrụ ndụ ihe nketa na-edobe catalase-peroxidase na katG gene mutation nwere ike ịkwalite njikọ nke mgbidi cell mycolic acid, na-eme ka nje bacteria na-eguzogide ọgwụ isoniazid.A na-ejikọta okwu KatG n'ụzọ na-adịghị mma na mgbanwe na INH-MIC, yana mbelata okpukpu abụọ na okwu katG na-ebute mmụba dị ntakịrị okpukpu abụọ na MIC.Ihe ọzọ na-akpata nguzogide isoniazid na ụkwara nta mycobacterium na-eme mgbe ntinye ntọala, nhichapụ ma ọ bụ ngbanwe na-eme na mpaghara InhA gene nke mycobacterium ụkwara nta.
Ọwa
| ROX | inhA (-15C>T) saịtị · |
| CY5 | katG (315G>C) saịtị |
| VIC (HEX) | IS6110 |
Nka nka
| Nchekwa | ≤-18℃ N'ọchịchịrị |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Sputum |
| CV | ≤5.0% |
| LoD | 1 × 103nje bacteria/ml |
| Nkọwapụta | Mmeghachi omume enweghị obe na ngbanwe nke saịtị anọ na-eguzogide ọgwụ (511, 516, 526 na 531) nke mkpụrụ ndụ rpoB na-abụghị oke nchọta nke ngwa nchọpụta ahụ. Ngwa ngwa: Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR ezigbo oge QuantStudio5 Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
