Mycobacterium ụkwara nta nke Isoniazid Mgbanwe Mgbanwe

Mycobacterium ụkwara nta nke Isoniazid Resistance Mutation egosipụtara

Nkọwa dị mkpirikpi:

Ngwa a dabara adaba maka nchọpụta qualitative nke isi ngbanwe saịtị na sputum sample mmadụ anakọtara site na Tubercle bacillus nti ndị ọrịa na-eduga mycobacterium ụkwara nta isoniazid resistance: InhA promoter region -15C>T, -8T>A, -8T>C;AhpC mpaghara nkwalite -12C>T, -6G>A;Mgbanwe homozygous nke KatG 315 codon 315G>A, 315G>C .

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HWTS-RT137 Mycobacterium Ụkwara nta nke Isoniazid Nguzogide Mgbanwe Nchọpụta Ngwa (Ọkpụkpọ Mgbaze)

Epidemiology

Mycobacterium ụkwara nta, obere oge dị ka Tubercle bacillus (TB), bụ nje nje na-akpata ụkwara nta.Ugbu a, ọgwụ mgbochi ụkwara nta nke mbụ a na-ejikarị gụnyere isoniazid, rifampicin na hexambutol, wdg. Ọgwụ mgbochi ụkwara nta nke abụọ gụnyere fluoroquinolones, amikacin na kanamycin, wdg. Ọgwụ ọhụrụ emepụtara bụ linezolid, bedaquiline na delamani, wdg. Otú ọ dị, n'ihi iji ọgwụ mgbochi ụkwara nta na-ezighị ezi na njirimara nke mgbidi cell nke ụkwara nta nke mycobacterium, ụkwara nta mycobacterium na-emepụta ọgwụ na-eguzogide ọgwụ mgbochi ụkwara nta, nke na-eweta nnukwu nsogbu na mgbochi na ọgwụgwọ ụkwara nta.

Ọwa

FAM	MP nucleic acid
ROX	Njikwa ime

Nka nka

Nchekwa	≤-18℃
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ụdị	sputum
CV	≤5%
LoD	Oke nchọpụta maka nje bacteria na-eguzogide ụdị isoniazid bụ 2x103 bacteria/ml, na njedebe nchọpụta maka nje bacteria bụ 2x103 bacteria/ml.
Nkọwapụta	a.Enweghị mmeghachi omume na-agafe agafe n'etiti genome mmadụ, mycobacteria ndị ọzọ na-abụghị tuberculous na oyi baa nke ngwa a chọpụtara.b.Achọpụtara saịtị ngbanwe nke mkpụrụ ndụ ihe nketa ndị ọzọ na-eguzogide ọgwụ na ụdị Mycobacterium ụkwara nta, dị ka nguzogide na-ekpebi mpaghara nke mkpụrụ ndụ rifampicin rpoB, na nsonaazụ nnwale ahụ egosighi nguzogide isoniazid, na-egosi enweghị mmeghachi omume cross.
Ngwa ndị ọdabara	Sistemụ PCR SLAN-96P ezigbo ogeBioRad CFX96 ezigbo oge PCR Systems LightCycler480® Real-Time PCR System

Usoro ọrụ

Ọ bụrụ na-eji Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (nke enwere ike iji Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) site na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. maka mmịpụta, tinye 200μL nke njikwa na-adịghị mma na nhazi sputum sample ga-anwale n'usoro, tinye 10μL nke n'ime njikwa iche iche n'ime njikwa na-adịghị mma, nhazi sputum sample ga-anwale, na-esote nzọụkwụ kwesịrị ime nke ọma dị ka ntuziaka mmịpụta.Ihe nlele nke ewepụtara bụ 200μL, na olu elution akwadoro bụ 100μL.