Mgbanwe nke Mycobacterium Tuberculosis INH
Aha ngwaahịa
Ngwa Nchọpụta Mgbanwe nke HWTS-RT137 Mycobacterium Tuberculosis INH (Mgbaze Curve)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa ụkwara nta nke Mycobacterium, n'oge na-adịghị anya dịka Tubercle Bacillus (TB), bụ nje bacteria na-akpata ụkwara nta. Ugbu a, ọgwụ mgbochi ụkwara nta nke a na-ejikarị eme ihe gụnyere INH, rifampicin na hexambutol, wdg. Ọgwụ mgbochi ụkwara nta nke abụọ gụnyere fluoroquinolones, amikacin na kanamycin, wdg. Ọgwụ ọhụrụ ndị a mepụtara bụ linezolid, bedaquiline na delamani, wdg. Agbanyeghị, n'ihi ojiji na-ezighi ezi nke ọgwụ mgbochi ụkwara nta na njirimara nke usoro mgbidi sel nke ụkwara nta nke mycobacterium, ụkwara nta mycobacterium na-emepụta iguzogide ọgwụ mgbochi ụkwara nta, nke na-eweta nnukwu ihe ịma aka na mgbochi na ọgwụgwọ ụkwara nta.
Ọwa
| FAM | MP nucleic acid |
| ROX | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | afụ |
| CV | ≤5% |
| LoD | Oke nchọpụta maka nje bacteria INH nke ụdị ọhịa bụ 2x103 bacteria/mL, oke nchọpụta maka nje bacteria ndị na-agbanwe agbanwe bụ 2x103 bacteria/mL. |
| Nkọwapụta | a. Enweghị mmeghachi omume n'etiti mkpụrụ ndụ ihe nketa mmadụ, nje bacteria ndị ọzọ na-abụghị ụkwara nta na nje pneumonia nke ngwa a chọpụtara.b. Achọpụtara ebe mgbanwe nke mkpụrụ ndụ ihe nketa ndị ọzọ na-eguzogide ọgwụ n'ime ụkwara nta Mycobacterium nke ụdị ọhịa, dị ka mpaghara na-ekpebi iguzogide nke mkpụrụ ndụ ihe nketa rifampicin rpoB, nsonaazụ nnwale ahụ egosighi iguzogide INH ọ bụla, na-egosi na ọ nweghị mmeghachi omume cross. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR SLAN-96P Oge-N'ezieSistemụ PCR oge-oge BioRad CFX96LightCycler480®Sistemụ PCR Oge-N'ezie |
Usoro Ọrụ
Ọ bụrụ na ejiri Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (nke enwere ike iji Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. mee ihe maka mwepụta, tinye 200μL nke njikwa na-adịghị mma na ihe nlele sputum a haziri ahazi ka a nwalee ya n'usoro, ma tinye 10μL nke njikwa dị n'ime iche iche n'ime ihe nlele sputum a haziri ahazi ka a nwalee ya, a ga-emekwa usoro ndị na-esote nke ọma dịka ntuziaka mwepụta si dị. Olu ihe nlele ewepụtara bụ 200μL, olu mwepụ akwadoro bụ 100μL.
