Mycobacterium ụkwara nta INH Mgbanwe
Aha ngwaahịa
Ngwa HWTS-RT137 Mycobacterium Tuberculosis INH Mgbanwe Mgbanwe (Ọkpụkpọ Mgbaze)
Epidemiology
Mycobacterium ụkwara nta, obere oge dị ka Tubercle bacillus (TB), bụ nje nje na-akpata ụkwara nta. Ka ọ dị ugbu a, ọgwụ mgbochi ụkwara nta nke mbụ a na-ejikarị gụnyere INH, rifampicin na hexambutol, wdg. Ọgwụ mgbochi ụkwara nta nke abụọ gụnyere fluoroquinolones, amikacin na kanamycin, wdg. Ọgwụ ọhụrụ emepụtara bụ linezolid, bedaquiline na delamani, wdg. Otú ọ dị, n'ihi iji ọgwụ na-adịghị mma nke ọgwụ mgbochi ụkwara nta na-emepụta ihe na-emepụta ihe na-emepụta ihe na-emepụta ihe na-eme ka ọ bụrụ ihe na-adịghị mma nke cell cell . mycobacterium ụkwara nta na-etolite ọgwụ na-eguzogide ọgwụ mgbochi ụkwara nta, nke na-eweta ihe ịma aka siri ike na mgbochi na ọgwụgwọ ụkwara nta.
Ọwa
| FAM | MP nucleic acid |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | sputum |
| CV | ≤5% |
| LoD | Oke nchọpụta maka nje bacteria INH ụdị anụ ọhịa bụ 2x103 bacteria/mL, na njedebe nchọpụta maka nje bacteria bụ 2x103 bacteria/ml. |
| Nkọwapụta | a. Enweghị mmeghachi omume na-agafe agafe n'etiti genome mmadụ, mycobacteria ndị ọzọ na-abụghị tuberculous na oyi baa nke ngwa a chọpụtara.b. Achọpụtara ebe ngbanwe nke mkpụrụ ndụ ihe nketa ndị ọzọ na-eguzogide ọgwụ na ụdị anụ ọhịa Mycobacterium ụkwara nta, dị ka nguzogide na-ekpebi mpaghara nke mkpụrụ ndụ rifampicin rpoB, na nsonaazụ ule ahụ egosighi nguzogide INH, na-egosi enweghị mmeghachi omume cross. |
| Ngwa ndị ọdabara | Sistemụ PCR SLAN-96P ezigbo ogeBioRad CFX96 ezigbo oge PCR SystemsLightCycler480®Sistemụ PCR dị adị |
Usoro ọrụ
Ọ bụrụ na-eji Macro & Micro-Test General DNA / RNA Kit (HWTS-3019) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) site na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. maka mmepụta ihe na-adịghị mma, tinyekwa 2000 na-adịghị mma maka nhazi nke μL. n'usoro, ma gbakwunye 10μL nke nchịkwa dị n'ime iche n'ime njikwa na-adịghị mma, a na-edozi sputum sample ka a ga-anwale, na usoro ndị na-esote kwesịrị ime nke ọma dịka ntuziaka mmịpụta. Ihe nlele nke ewepụtara bụ 200μL, na olu elution akwadoro bụ 100μL.
