Mycobacterium ụkwara nta Rifampicin na-eguzogide

Nkọwa dị mkpirikpi:

Ngwa a dabara adaba maka nchọpụta qualitative nke homozygous mutation na mpaghara 507-533 amino acid codon nke mkpụrụ ndụ rpoB nke na-akpata Mycobacterium ụkwara nta rifampicin.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-RT074A-Mycobacterium ụkwara nta nke Rifampicin Nchọpụta Nguzogide (Fluorescence PCR)

Epidemiology

A na-eji Rifampicin na-agwọ ndị ọrịa ụkwara nta nke akpa ume kemgbe afọ 1970, ma nwee mmetụta dị ukwuu.Ọ bụ nhọrọ mbụ iji belata ọgwụgwọ ọgwụgwọ nke ndị ọrịa ụkwara nta.Ihe na-akpata nguzogide Rifampicin bụ ngbanwe nke mkpụrụ ndụ ihe nketa rpoB.Ọ bụ ezie na ọgwụ ọhụrụ na-egbochi ụkwara nta na-apụta mgbe nile, na ịdị irè ụlọ ọgwụ nke ndị ọrịa ụkwara nta na-aga n'ihu na-agakwa n'ihu, a ka nwere ụkọ ọgwụ mgbochi ụkwara nta, na ọdịdị nke iji ọgwụ na-enweghị isi eme ihe na ụlọ ọgwụ dị oke elu.N'ụzọ doro anya, ọrịa ụkwara nta nke Mycobacterium nke ndị ọrịa nwere ụkwara nta akpa ume enweghị ike igbu kpamkpam n'oge kwesịrị ekwesị, nke na-emecha na-eduga n'ọkwa dị iche iche nke nguzogide ọgwụ n'ime ahụ onye ọrịa, na-agbatị usoro nke ọrịa ahụ, na-abawanye ohere nke ọnwụ nke onye ọrịa.Ngwa a dabara adaba maka nyocha inyeaka nke ọrịa ụkwara nta nke Mycobacterium na ịchọpụta mkpụrụ ndụ ihe mgbochi rifampicin, nke na-enyere aka ịghọta nguzogide ọgwụ nke ụkwara nta mycobacterium nke ndị ọrịa butere, na inye ụzọ inyeaka maka nduzi ọgwụ ụlọ ọgwụ.

Epidemiology

 

Aha ebumnuche Onye nta akụkọ Quencher
Nchekwa mmeghachi omumeA Nchekwa mmeghachi omumeB Nchekwa mmeghachi omumeC
rpoB 507-514 rpoB 513-520 IS6110 FAM Ọ dịghị
rpoB 520-527 rpoB 527-533 / CY5 Ọ dịghị
/ / Njikwa ime HEX (VIC) Ọ dịghị

 

Nka nka

Nchekwa

≤-18℃ N'ọchịchịrị

Ndụ nchekwa

Ọnwa 9

Ụdị ụdị

Sputum

CV

5%

LoD

Ụdị anụ ọhịa rifampicin na-eguzogide: 2x103nje bacteria/ml

mutant homozygous: 2x103nje bacteria/ml

Nkọwapụta

Ọ na-achọpụta ụkwara nta mycobacterium nke anụ ọhịa na ebe ngbanwe nke mkpụrụ ndụ ihe nketa ọgwụ ndị ọzọ dị ka katG 315G>C\A, InhA-15C>T, nsonaazụ ule egosighi nguzogide rifampicin, nke pụtara na ọ dịghị cross-reactivity.

Ngwa ngwa:

SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.)

Usoro ọrụ


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