Mycobacterium ụyọkọm nucleic acid na rifamplicin iguzogide
Aha
HWT-RT074B-Mycobactosis Truberculosis acid acid na rifammicin na-eguzogide ihe Kit (na-agbaze usoro)
Asambodo
CE
Mpidermiology
Mycobactecrium ụkwara nta, n'oge na-adịghị anya dị ka tubercle bicillus, TB, nje nje patkpanic nke na-akpata ụkwara nta. Ugbu a, ọgwụ mgbochi na-ejikarị bụ ọgwụ na-egbu egbu, Rifamicin na Hexambin, wdg. . Kaosinadị, n'ihi ihe na-ezighi ezi Ọgwụ mgbochi na njiri mara ụlọ mgbidi courctosis nke mycobactosis, mycobactosis na-eme ka ọgwụ mgbochi ọrịa na-eme ihe ike, nke na-eweta oke nsogbu na ọgwụgwọ nke ụkwara ụyọkọ.
A na-eji Rifampicin mee ihe n'ọtụtụ ọgwụ a purnomosis ndị ọrịa kemgbe ngwụsị afọ 1970, ma nwee mmetụta dị ukwuu. Ọ bụ nhọrọ mbụ iji belata chemotherapy nke ndị ọrịa ụkwara nta. RIFAMMICIN Ndogide na-akpata site na mmụba nke RPOB Gene. Ọ bụ ezie na ọgwụ mgbochi nje na-apụtakarị, na arụmọrụ nke ọrịa ụkwara akwara agbachiwo anya, a ka nwere ọgwụ mgbochi ọrịa, na ihe na-enweghị isi na-enweghị isi na ụlọ ọgwụ dị elu. N'ụzọ doro anya, mycobosis ụyọstactosis na ndị ọrịa nwere nku purculonish enweghị ike igbu egbu oge, nke na-ebute ogo ọgwụgwụ nke ọrịa ahụ, ma na-eme ka ọ bụrụ ihe ize ndụ nke ọnwụ onye ọrịa.
Chaneelu
| Chaneelu | Ọwa na fluorophores | Mmeghachi omume na-eme ihe a | Mmeghachi omume na-arụ ọrụ b | Mmeghachi omume na-eme ihe c |
| Ato ey | Reporter: UM, onye na-ekwu okwu: onweghi | RPOB 507-514 | RPOB 513-520 | 38KD na IS6110 |
| Chanel cy5 | Reporter: Cy5, onye na-azụ ahịa: onweghị | RPOB 520-527 | RPOB 527-533 | / |
| Hex (vic) ọwa | Reporter: Hex (VIC), onye na-apụ apụ: ọ dịghị | Nchịkwa ime | Nchịkwa ime | Nchịkwa ime |
Nrụta Nka na ụzụ
| Storaji | ≤-18 ℃ n'ọchịchịrị |
| Ebe ndu | 12 ọnwa |
| Ụdị ụdị | Sputum |
| CV | ≤5.0% |
| Loo | bacteriatium Tubercucterium 50 bacteria / ml ụdị RIFAMPICING-na-eguzogide oke: 2x103nje na ml Domozygous: 2x103nje na ml |
| Nnoshi | Ọ na-achọpụta ụdị ụycucterium ụyọkọ ụkwara ụyọkọ na saịtị na-eguzogide mkpụrụ ndụ ihe ndị ọzọ dị ka Katg 315G> Nsonaazụ na-egosi enweghị nguzogide rifamicin, nke pụtara na ọ nweghị ihe na-adịghị mma. |
| Ngwá ọrụ dị ala: | Slan-96p ezigbo oge pcr Beired cfx96 ezigbo oge pcr sistemụ Lightcycler480® ezigbo oge pcr sistemụ |
Na-arụ ọrụ
If use the Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (which can be used with Macro & Micro-Test Automatic Nucleic Acid (HWT-3006C, HWT-3006B)) ma ọ bụ ọrịa macro & Micro-ule Nchịkọta DNA / HNA - HWT-3022-50) Site na Jiangsu Macro & Micro-Nnwale Med-Tech Spotum ihe nlele ga-anwale n'usoro, ma tinye 10μL nke nchịkwa dị iche iche na njikwa dị mma, nchịkwa na-adịghị mma na sputum sputum a ga-anwale, na usoro ndị ahụ ga-emerịrị nke ọma, a ga-arụ ọrụ nke ọma dabere na mmịpụta Ntuziaka. Olu mmepụta ahụ wepụtara bụ 200μL, na olu ure atụ aro bụ 100μl.
