Nnwale EasyAmp site na Macro na Micro—- Ngwa Mgbakwunye Fluorescence Isothermal nke a na-ebugharị ebugharị nke dakọtara na LAMP/RPA/NASBA/HDA

Arụmọrụ Dị Mma & Ngwa zuru oke

Easy Amp, site na teknụzụ nke immụba nke sothermal nucleic acida na-egosipụta ya na oke dị elummetụta uche namkpụmkpụmmeghachi omumeoge na-enweghị ihe achọrọ maka usoro mgbanwe okpomọkụ. Ya mere, o nwereịpụtaeddị kaọtụtụteknụzụ kachasị amasị maka nnwale ngwa ngwa nke nucleic acid.Na-enweghị mgbagwoju anya tsistemụ ịgba ígwè hermal, ngwaọrụ nkwalite isothermal dị mfe ma dị mfe ibugharịnwere ike imeju afọ ojujuusoro mmeghachi omume dum.

Macro na Micro-Test emepụtala onwe hama maliteEasyAmp,ngwa mgbakwunye fluorescent isothermal amplification nke a na-ebugharị ebugharịmaka ngwa dị iche iche.

Ugbu a,Mfe Amp a kwadoro ka ọ bụrụdakọtara na ọtụtụ usoro mmụba okpomọkụ: Mgbakwunye Isothermal Loop-Mediated (LAMP), Mgbakwunye Polymerase Recombinase (RPA/RAA/MIRA), Mgbakwunye Acid Sequence Based Nucleic (NASBA), na Mgbakwunye Helicase Dependent (HDA)Ọ ga-abụ ngwa ọzọ dị mma maka nnwale nucleic acid ngwa ngwa yana ojiji sara mbara karị.

· Mgbakwunye Isothermal nke ejiri aka mee (LEMP):Mmụba na60-65°Cna dummmeghachi omumeusoroemechara ya n'ime nkeji iri atọ.Ịhụ eserese nke 1 maka mmụba yaụkpụrụ:

 

Fig 1. Ụkpụrụof oriọna 

· Mmelite Mmelite Polymerase (RPA/RAA/MIRA):Mmụba na37°C-42°Cna dummmeghachi omumeusoroemechara n'ime nkeji iri ruo iri atọ.Lee foto nke abụọ maka yaụkpụrụ mmụba:

Fig 2. Ụkpụrụof Mmelite Mmelite Polymerase Recombinase

·Mgbakwunye Dabere na Usoro Acid Nucleic (NASBA):Mmụba na42°Cna dummmeghachi omumeusoroemechara n'ime nkeji 40-120.Lee foto nke atọ maka yaụkpụrụ mmụba:

Fig 3. Ụkpụrụof Mmụba Dabere na Usoro Acid Nucleic

· Mmụba nke Helicase Dabere (HDA):Mmụba na65°Cna dummmeghachi omumeusoroemechara n'ime nkeji 70-90.Lee foto nke 4 maka yaụkpụrụ mmụba:

Fig4. Ụkpụrụof Mgbakwunye Dabere na Helicase

· EasyAmpmee ka o kwe omumeỌ na-eme ka DNA na RNA dịkwuo mma ma kwụsie ike site na iji usoro isothermal anọ a kpọtụrụ aha n'elu. Ọ na-enweta mmetụta mmụba ruo 200 copies/mL ma na-ahụ na nkọwapụta dị elu na-enweghị mmeghachi omume cross-reactivity na nkwalite ebumnuche.(Ie sere na eserese 5 ruo 7).

Nchọpụta RNA Nchọpụta DNA

Fig5. Ikpo okwu RAA/MIRA

Nchọpụta RNA Nchọpụta DNA

Fig6. Ikpo okwu LAMP

 

Nchọpụta RNA Nchọpụta DNA

Fig7. NASBA Platform

Uru Ngwaahịa:

Ọsọ ọsọ

· EPIAteknụzụ na-achọpụtansonaazụ dị mmaachọpụtara n'ime nkeji 5.

· Ọrụ dị mfe site na mwepụ otu nzọụkwụmaka mmụba na ngwa egwu.

Dị irè

· Modulu anọ nọọrọ onwe ha maka nnwale dị iche iche n'otu oge, n'otu oge maka ihe dị iche iche anọụdị nnwales.

· Modulu ọ bụla nwere ike inyocha ihe nlele anọ n'otu oge.

· Abụọ/Anọfluorescenceọwamaka nchọpụta ọtụtụ ihe mgbaru ọsọ sara mbara.

Ndakọrịta

· Dakọtara naọtụtụikpo okwu isothermal dịka LAMP, RPA, NASBA, HDA.

· Emebere ya maka mmụgharị okpomọkụ dị elu mgbe e degharịrị ya n'ọnọdụ dị ala.

· Hazie onwe gịikeiji mezuo mkpa ndị ahịa kpọmkwem.

Dị mfe ojiji

· Pimewe gburugburu(290×245×128mm, 2kg) maka ụdị dị iche iche nke ndị agadi.

· Ihe mmetụ aka agba 7-inchmaka ọrụ dị mfe na ihe osise nsonaazụ doro anya.

· Igwe nbipute okpomọkụ arụnyere n'ime ya maka mbipụta nsonaazụ ozugbo.

· Igwe nyocha agbakwunyere maka ịgụ ihe nchọpụta akpaka.