EasyAmp site na Macro & Micro Test—-Ngwaọrụ Amplification Isothermal Fluorescence Dakọtara na LAMP/RPA/NASBA/HDA

Ọmarịcha arụmọrụ & ngwa sara mbara

Easy Amp, site na teknụzụ nke isothermal nucleic acid amplificationa na-egosipụta na eluuche nankenkemmeghachi omumeoge na-enweghị chọrọ maka usoro mgbanwe okpomọkụ. Ya mere, o nwereemeputaeddị kaọtụtụTeknụzụ masịrị maka nyocha ngwa ngwa nucleic acid.Enweghị mgbagwoju anya tSistemụ ịgba ígwè hermal, ngwaọrụ nkwalite isothermal dị mfe ma dị mfenwere ike imeju iheusoro mmeghachi omume dum.

Macro & Micro-Test ewepụtala onwe yana ulo oruEasyAmp,ngwá ọrụ amplification isothermal fluorescentmaka ngwa dị iche iche.

Ugbu a, ndịAmp dị mfe akwadola ịbụdakọtara na ọtụtụ usoro nkwalite isothermal: Loop-Mediated Isothermal Amplification (LAMP), Recombinase Polymerase Amplification (RPA/RAA/MIRA), Nucleic Acid Sequence Based Amplification (NASBA), na Helicase Dependent Amplification (HDA).Ọ ga-abụ ngwa ọzọ na-egbutu ọnụ maka nnwale ngwa ngwa nucleic acid nwere ngwa ka ukwuu.

Ọganihu Isothermal Amụma (LAMP):Amplification na60-65°Cna dummmeghachi omumeusoroEmechara n'ime nkeji iri atọ.Ịhụ foto 1 maka mmụba yaụkpụrụ:

 

Fig 1. Ụkpụrụof oriọna 

Recombinase Polymerase Amplification (RPA/RAA/MIRA):Amplification na37°C-42°Cna dummmeghachi omumeusoroemechara na nkeji 10-30.Ịhụ foto 2 maka yaụkpụrụ mmụba:

Fig 2. Ụkpụrụof Recombinase Polymerase Amplification

·Usoro Nkwalite Nucleic Acid (NASBA):Amplification na42°Cna dummmeghachi omumeusoroemechara na 40-120 nkeji.Ịhụ foto 3 maka yaụkpụrụ mmụba:

Fig 3. Ụkpụrụof Usoro Nucleic Acid Amplification dabere

Ọganihu na-adabere na helicase (HDA):Amplification na65°Cna dummmeghachi omumeusoroEmechara na 70-90 nkeji.Ịhụ foto 4 maka yaụkpụrụ mmụba:

Fig4.Ụkpụrụof Mmụba dabere na helicase

· EasyAmpmee kaNgosipụta nke ọma na nkwụsi ike maka nchọpụta DNA na RNA site na iji usoro isothermal anọ ahụ a kpọtụrụ aha n'elu.Ọ na-enweta mmetụta mmụba ruo 200 mbipụta / mL ma hụ na nkọwapụta dị elu na-enweghị cross-reactivity na nkwalite ebumnuche..(Iegosipụtara na foto 5 ruo 7).

Nchọpụta DNA Nchọpụta RNA

Fig5.RAA/MIRA Platform

Nchọpụta DNA Nchọpụta RNA

Fig6.Platform LAMP

 

Nchọpụta DNA Nchọpụta RNA

Fig7.NASBA Platform

Uru ngwaahịa:

Ngwa ngwa

· EPIAteknụzụ ghọtaranti utịpachọpụtara na 5 min.

· Ọrụ dị mfe na mmịpụta otu nzọụkwụmaka ngwa ngwa na ngwa.

Oru oma

4 modul nke onwe maka ihe achọrọ, nnwale n'otu oge nke 4 dị iche icheụdị ules.

Modul nke ọ bụla nwere ike nyochaa nlele anọ n'otu oge.

· Abụọ/Quadfluorescenceọwamaka nchọpụta ọtụtụ ebumnuche.

Ndakọrịta

· Dakọtara naotutuikpo okwu isothermal dị ka LAMP, RPA, NASBA, HDA.

· Ezubere maka nkwalite okpomọkụ dị elu biputere ntụgharị ntụgharị ihu igwe dị ala.

· Hazieikeiji gboo mkpa ndị ahịa.

Dị mfe ojiji

· Portable imewe(290×245×128mm, 2kg) maka dị iche iche ngwa senarios.

· 7-inch agba touchscreenmaka ịrụ ọrụ dị mfe yana nkọwa nsonaazụ doro anya.

Ihe nbipute ọkụ arụnyere n'ime ya maka ibipụta nsonaazụ ozugbo.

· Igwe nyocha jikọtara ọnụ maka ọgụgụ ihe nchọpụta akpaka.