—— Ọmụmụ mmekorita nke Zhejiang CDC, Macro & Micro-Test, na China CDC bipụtara na Frontiers in Cellular and Infection Microbiology
Nchịkọta Ọmụmụ Ihe
Na Mee 2026, Frontiers in Cellular and Infection Microbiology (JCR Q1, IF ≈ 4.6) bipụtara akwụkwọ nke Zhejiang Provincial Center for Disease Control and Prevention (Zhejiang CDC) duziri, ya na ndị otu bioinformatics sitere na Beijing Macro & Micro-Test Bio-Tech Co., Ltd. na National Institute for Communicable Disease Control and Prevention (China CDC) dịka ndị edemede. A kpọrọ ọmụmụ ihe a:
"Nyocha njirimara na nhazi nke ụdị Brucella abortus asaa na Zhejiang, China."
Ọmụmụ ihe a na-anọchite anya nyocha mbụ nke usoro, nke dabere na mkpụrụ ndụ ihe nketa nke Brucella abortus (B. abortus) na Zhejiang Province, China. Ndị otu ahụ nyochachara ihe dị iche iche asaa a nakọtara site na 2015 ruo 2025 (ụdị mmadụ anọ na ụdị ehi atọ sitere na Jinhua, Quzhou, na Ningbo). Nchọpụta ndị a na-enye ihe akaebe genomic maka mmalite na ụzọ mbufe nke "ụdị ugwu a na-ahụkarị" na mpaghara ọrịa na-efe efe na ndịda nke ọwụwa anyanwụ China.
Ndabere na Mkpa
Brucellosis bụ ọrịa anụmanụ nke nje bacteria nke ụdị Brucella na-akpata. Brucella abortus na-ebutekarị ehi mana ọ nwekwara ike ibute ọrịa n'ime mmadụ. Na China, brucellosis na-egosi nnukwu ọdịiche dị n'etiti ala: ọnụọgụgụ kachasị elu na-apụta na mpaghara ugwu (dịka ọmụmaatụ, Inner Mongolia, Shanxi, Heilongjiang). N'aka nke ọzọ, ógbè ndịda, gụnyere Zhejiang, bụ nke Brucella melitensis na-achị n'oge gara aga, ebe a kọrọ na ọ bụ naanị mmadụ ole na ole nwere B. abortus. Nha nhata mpaghara a na-eme ka njirimara mkpụrụ ndụ ihe nketa na nchọpụta isi mmalite nke B. abortus na Zhejiang bụrụ ihe kacha mkpa maka ahụike ọha na eze.
Ụzọ na Nchọpụta Ndị Dị Mkpa
Ndị otu nnyocha ahụ nakweere usoro nwere ọtụtụ akụkụ nke jikọtara bayoloji molekul na bioinformatics:
1.Nchọpụta ọrịa na ụdị isi
BCSP-31 gene PCR na AMOS-PCR kwadoro na ihe asaa niile bụ B. abortus.
Ntinye usoro Multilocus (MLST) nke dabere na mkpụrụ ndụ ihe nketa nlekọta ụlọ itoolu gosiri na ihe niile dị iche iche bụ nke ụdị usoro ST2, nke na-egosi oke otu mkpụrụ ndụ ihe nketa n'etiti ụdị B. abortus na-agagharị na Zhejiang.
2.Nkọwa nke mkpụrụ ndụ ihe nketa dum
E mere usoro mkpụrụ ndụ ihe nketa zuru oke na ikpo okwu Illumina NovaSeq. Nyocha nkezi nke njirimara nucleotide (ANI) gosiri na ihe ndị Zhejiang kewara ihe ruru 99.99% na ụdị ntụaka B. abortus 544.
Nnyocha nke mkpụrụ ndụ ihe nketa (pan-genome) gosiri na e nwere ọtụtụ mmadụ echekwara nke ọma: achọpụtara mkpụrụ ndụ ihe nketa isi 3,084, yana naanị mkpụrụ ndụ ihe nketa shell iri, a chọpụtaghịkwa mkpụrụ ndụ ihe nketa dị nro ma ọ bụ nke igwe ojii.
3.Nkọwa mkpụrụ ndụ ihe nketa na-eguzogide nje na nje bacteria
E buru amụma na ngụkọta ihe 68 metụtara ọrịa nje na-efe efe, na-ekpuchi ụzọ ochie dịka LPS biosynthesis, sistemụ nzuzo T4SS, na sistemụ nchịkwa ihe abụọ nke BvrR-BvrS. Ihe pụrụ iche bụ na ihe niile e kewapụrụ enweghị mkpụrụ ndụ ihe nketa adhesin bmaA na btaF. Nyocha mkpụrụ ndụ ihe nketa iguzogide chọpụtara naanị mkpụrụ ndụ mprF dị na nchekwa data CARD, na-enweghị ihe ndị ọzọ na-ekpebi iguzogide.
4. Ndozigharị na nchọpụta nke phylogenetic
Nnyocha nke mkpụrụ ndụ ihe nketa nke otu nucleotide (cgSNP) tinyere ihe ndị dị na Zhejiang n'otu ebe kpọmkwem n'osisi phylogenetic zuru ụwa ọnụ. Nsonaazụ ya gosiri na ụdị Zhejiang na-etolite otu monophyletic yana ụdị sitere na Russia, Mongolia, na ọtụtụ ógbè ndị dị n'ebe ugwu China (Ningxia, Heilongjiang, Inner Mongolia, Hebei, Gansu, Beijing). Otu a kewara ọzọ n'ime obere klaasị atọ dị iche iche (Clade 1–3), na-egosi ọtụtụ ihe omume mmalite onwe ha.
Mmechi na ihe ndị ọ pụtara
Ọmụmụ ihe a na-enye data genomic mbụ dị oke nkenke nke B. abortus na Zhejiang Province ma na-enye ọtụtụ nkwubi okwu dị mkpa:
- Clear ndabere mkpụrụ ndụ ihe nketa– Ụdị B. abortus nke na-agagharị na Zhejiang bụ nke ST2, a na-echekwa ha nke ọma n'ihe gbasara mkpụrụ ndụ ihe nketa, ma na-anọchite anya ụdị brucellosis ehi a na-ahụkarị.
2. Evimgbasa ozi mpaghara gafere– Nyocha nke phylogenetic anaghị akwado ịdị adị nke usoro ọmụmụ nke onwe ya na Zhejiang. Kama nke ahụ, data ahụ na-egosi nke ọma na ụdị ndị a sitere na ugwu China ma nwee ike ịkekọrịta ndabere evolushọn na ụdị ndị sitere na Russia na Mongolia. Ọnụnọ nke obere klaasị atọ na-egosi ọtụtụ ihe omume mmalite dị iche iche.
3. Mmetụta ahụike ọha na eze– Nchọpụta ndị a na-egosi uru nke nlekota genomic maka ọrịa brucellosis ọbụlagodi na mpaghara ndị na-adịghị ahụkebe dịka Zhejiang. Ọ bụ ezie na ọnụọgụgụ ndị ọrịa dị ugbu a dị ala, ngwaọrụ dị elu dịka cgSNP nwere ike ịchọpụta ebe ọrịa si apụta ma nye ihe akaebe sayensị iji kwụsị usoro nnyefe nke metụtara njem anụ ụlọ n'etiti mpaghara.
Ọrụ a abụghị naanị na ọ na-ejupụta oghere nyocha na Zhejiang Province kamakwa ọ na-enye data mmalite ọhụrụ maka nlekota nje na nyocha ihe egwu nke brucellosis na mpaghara Yangtze River Delta.
Ozi Akwụkwọ:
Yang, Y., Shi, X., Chen, J., Wang, L., Wu, Z., Yao, W., … & Wu, B. (2026). Nchọpụta na nyocha nke ụdị Brucella abortus asaa na Zhejiang, China. Frontiers in Cellular and Infection Microbiology, 16, 1758965.
Oge ozi: Juun-10-2026