Clostridium difficile toxin A/B gene (C.diff)
Aha ngwaahịa
Ngwa HWTS-OT031A Nchọpụta Nucleic Acid maka Clostridium difficile toxin A/B gene (C.diff) (Fluorescence PCR)
Asambodo
CE
Epidemiology
Clostridium difficile (CD), gram-positive anaerobic sporogenic Clostridium difficile, bụ otu n'ime ihe ndị na-akpata ọrịa nrịanrịa nke nosocomial.N'ụlọ ọgwụ, ihe dị ka 15% ~ 25% nke afọ ọsịsa metụtara antimicrobial, 50% ~ 75% nke colitis metụtara antimicrobial na 95% ~ 100% nke pseudomembranous enteritis bụ ọrịa Clostridium siri ike (CDI).Clostridium difficile bụ ọrịa na-akpata ọnọdụ, gụnyere ụdị nsi na ụdị anaghị egbu egbu.
Ọwa
| FAM | tcdAmkpụrụ ndụ ihe nketa |
| ROX | tcdBmkpụrụ ndụ ihe nketa |
| VIC/HEX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | stool |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/ml |
| Nkọwapụta | jiri ngwa a chọpụta nje ndị ọzọ na-akpata eriri afọ dị ka Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile na-abụghị pathogenic strains, Adenovirus, rotavirus, norovirus, influenza A virus, influenza virus na human gefluenza virus. DNA, nsonaazụ ya niile adịghị mma. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 PlusFQD-96A,HangzhouTeknụzụ bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1.
Tinye 180μL nke lysozyme buffer na precipitate (gbanye lysozyme na 20mg / mL na lysozyme diluent), pipette iji jikọta nke ọma, na nhazi na 37 ° C maka ihe karịrị nkeji 30. Were 1.5mL nke RNase / DNase-free tube centrifuge tube, ma tinye180μL nke njikwa dị mma na njikwa na-adịghị mma n'usoro.Tinye10μL nke njikwa ime n'ime ihe nlele a ga-anwale, njikwa ziri ezi na njikwa na-adịghị mma n'usoro, wee jiri Nucleic Acid Extraction or Purification Reagent (YDP302) nke Tiangen Biotech (Beijing) Co., Ltd. maka mmịpụta DNA na-esote, yana biko soro ntuziaka maka ojiji maka usoro ụfọdụ.Jiri DNAse/RNase H2O maka elution, na olu elution akwadoro bụ 100μL.
Nhọrọ 2.
Were 1.5mL nke RNase/DNase centrifuge tube na-enweghị, ma tinye 200μL nke njikwa dị mma na njikwa adịghị mma n'usoro.Tinye10μL nke njikwa ime n'ime ihe nlele a ga-anwale, njikwa dị mma na njikwa na-adịghị mma n'usoro, wee jiri Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004- 96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).Ekwesịrị ime mmịpụta ahụ n'ụzọ siri ike dabere na ntuziaka maka ojiji, na oke elution akwadoro bụ 80μL.
