Mkpụrụ ndụ ihe nketa Clostridium siri ike nsí A/B (C. diff)
Aha ngwaahịa
Ngwa Nchọpụta Nucleic Acid HWTS-OT031A maka mkpụrụ ndụ ihe nketa A/B nke Clostridium siri ike (C.diff) (Fluorescence PCR)
Asambodo
CE
Ọmụmụ ihe gbasara ọrịa na-efe efe
Clostridium difficile (CD), nke bụ gram-positive anaerobic sporogenic Clostridium difficile, bụ otu n'ime nje ndị bụ isi na-akpata ọrịa eriri afọ nosocomial. N'ụlọ ọgwụ, ihe dị ka 15% ~ 25% nke afọ ọsịsa metụtara nje bacteria, 50% ~ 75% nke colitis metụtara nje bacteria na 95% ~ 100% nke pseudomembranous enteritis bụ ọrịa Clostridium difficile (CDI). Clostridium difficile bụ nje nwere ike ibute ọrịa, gụnyere ụdị toxin na ụdị ndị na-anaghị egbu egbu.
Ọwa
| FAM | tcdAmkpụrụ ndụ ihe nketa |
| ROX | tcdBmkpụrụ ndụ ihe nketa |
| VIC/HEX | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | oche |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Nkọwapụta | Jiri ngwa a chọpụta nje ndị ọzọ dị n'ime eriri afọ dịka Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, influenza A virus, influenza B virus na human genomic DNA, ihe ndị a niile adịghị mma. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P nke Oge-N'ezie (Hongshi Medical Technology Co., Ltd.) LightCycler®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị (FQD-96A,Hangzhouteknụzụ biore) MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya (Suzhou Molarray Co., Ltd.) Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Nhọrọ nke 1.
Tinye 180μL nke ihe nchekwa lysozyme na precipitate (gwakọta lysozyme ahụ ruo 20mg/mL na lysozyme diluent), pipette ka ọ gwakọta nke ọma, wee hazie ya na 37°C ruo ihe karịrị nkeji iri atọ. Were 1.5mL nke tube centrifuge na-enweghị RNase/DNase, wee tinye ya180μL nke njikwa dị mma na njikwa na-adịghị mma n'usoro. Tinye10μL nke njikwa ime na ihe nlele ahụ a ga-anwale, njikwa dị mma, na njikwa na-adịghị mma n'usoro, ma jiri Nucleic Acid Extraction or Purification Reagent (YDP302) nke Tiangen Biotech (Beijing) Co., Ltd. maka mwepụta DNA ihe nlele na-esote, biko soro ntuziaka maka ojiji maka usoro ụfọdụ. Jiri DNase/RNase na-enweghị H2O maka iwepụ mmiri, olu nhicha mmiri akwadoro bụ 100μL.
Nhọrọ nke abụọ.
Were 1.5mL nke tube centrifuge na-enweghị RNase/DNase, wee tinye 200μL nke njikwa dị mma na njikwa adịghị mma n'usoro.10μL nke njikwa ime na ihe nlele ahụ ka a nwalee, njikwa dị mma, na njikwa adịghị mma n'usoro, ma jiri Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). A ga-eme mwepụta ahụ dịka ntuziaka maka ojiji si dị, olu mgbapụta akwadoro bụkwa 80μL.
