Mmehie Mmechi Mgbanwe A / B Gene (C.Diff)
Aha
HWT-ot031
Asambodo
CE
Mpidermiology
Mmechi Mmehie di iche (CD), a na-eme ihe na-eme ka mmiri dị iche iche dị iche iche dị iche, bụ otu n'ime nje ndị bụ isi na-ebute imi na-efe efe. Ọrịa 50% nke ọrịa Antimicrobial-metụtara ọgwụ mgbochi na 95% ~ 100% nke Pserumiumble intencile ọrịa (CDI) kpatara ya. Mmechi Mmechi bụ nje na-enweghị ọnọdụ, gụnyere ụdị toxigenic na ụdị na-abụghị nke.
Chaneelu
| M | tcdaozizi |
| Rox | tcdbozizi |
| Vic / hex | Nchịkwa ime |
Nrụta Nka na ụzụ
| Storaji | ≤-18 ℃ |
| Ebe ndu | 12 ọnwa |
| Ụdị ụdị | stool |
| Tt | ≤38 |
| CV | ≤5.0% |
| Loo | 200Cfu / ml |
| Nnoshi | Jiri Kit a iji chọpụta nje ndị ọzọ dị ka Escheris Cori, Shedococcus Aous, Amenovarium Strahaic na-enweghị nje, Virtous, na-emetụta nje, cerfenza b virus na Chyomic DNA, Nsonaazụ bụ ihe ọjọọ. |
| Ngwá agha | Ngwa Biosystems na-etinye 7500 ezigbo oge pcr Ngwa Biosystems na-etinye 7500 ngwa ngwa usoro pcr Nke quagruo®5 ezigbo oge pcr Oge Slan-96p Real-96p Systems (Hongshili Medical Teknụzụ CO., LTD.) Lightcycler®480 ezigbo oge pcr sistemụ Linegene 9600 gbakwunyere ezigbo oge Pcr nchọpụta (FQD-96A,HangzhouTeknụzụ Bioeer) Ma-6000 Real-Oge gwọike Cycler (Suzhou Molerray Co., LTD.) Beired cfx96 ezigbo oge pcr sistemụ Beired cfx opus 96 Real System |
Na-arụ ọrụ
Nke 1.
Tinye 180μL nke lysozyme na-echekwa ihe na-aga nke ọma (ngụkọta lysozyme ruo 20mg / ml na lySOzyme turke, ma tinye180μl nke njikwa na njikwa na-adịghị mma n'usoro. Igbako10na njikwa ime ihe ahụ na-anwale, njikwa dị mma, yana njikwa recleic (YDD302) site na ndọtị Nọọsụ Biko kpachapụrụ ntuziaka maka iji maka usoro ụfọdụ. Jiri Dnase / Rnase Free H2O N'ihi na ntinye aka, na olu ure a tụrụ aro dị 100μl.
Nke 2.
Were 1.5ml nke Rnase / Dnase-Free centte, ma tinye 200μL nke njikwa ziri ezi na njikwa na-adịghị mma n'usoro. Igbako10Ohere nke ime ihe na-eme ka a nwalee, njikwa ziri ezi, yana njiri mara mma na-ebu / RBE-3004-38, HWT-3004- 96) na Macro & Micro-Nnwale Acctic acid na-emepụta nke Exctor (HWT-3006). A ga-eme ka mmịpụta ahụ rụọ ọrụ siri ike na ntuziaka maka ojiji, yana olu usọ akwadoro bụ 80μl.
