Ejikọtara Ọrịa iku ume
Aha ngwaahịa
HWTS-RT183-Ngwungwu Nchọpụta Ihe Nchọpụta Mgbakọ (Fluorescence PCR)
Epidemiology
Ọrịa Corona Virus 2019, nke akpọrọ 'COVID-19', na-ezo aka na oyi baa nke ọrịa SARS-CoV-2 kpatara. SARS-CoV-2 bụ coronavirus nke β genus. COVID-19 bụ ọrịa na-efe efe na-efe efe na-efe efe na-efe efe, ndị mmadụ na-enwekarịkwa ike. Ka ọ dị ugbu a, isi mmalite nke ọrịa bụ ndị ọrịa butere site na 2019-nCoV, na ndị butere ọrịa asymptomatic nwekwara ike bụrụ isi mmalite nke ọrịa. Dabere na nyocha ọrịa ọrịa ugbu a, oge nnabata bụ ụbọchị 1-14, ọkachasị ụbọchị 3-7. Ahụ ọkụ, ụkwara akọrọ na ike ọgwụgwụ bụ isi ihe ngosi. Ndị ọrịa ole na ole nwere ihe mgbaàmà dị ka imi imi, imi imi, akpịrị akpịrị, myalgia na afọ ọsịsa, wdg. Influenza, nke a na-akpọkarị 'flu', bụ nnukwu ọrịa na-efe efe nke iku ume nke nje influenza kpatara. Ọ na-efe efe nke ukwuu. A na-ebutekarị ya site na ụkwara na imi. Ọ na-apụtakarị na mmiri na oyi. A na-ekewa nje virus influenza n'ime influenza A (IFV A), influenza B (IFV B), na influenza C (IFV C) ụdị atọ, ha niile bụ nje virus na-egbuke egbuke, na-ebute ọrịa mmadụ karịsịa maka nje nje A na B, ọ bụ nje RNA nwere otu eriri, nke kewara ekewa. Influenza A bụ nnukwu ọrịa iku ume, gụnyere H1N1, H3N2 na ụdị ndị ọzọ, nke na-enwekarị mmụgharị na ntiwapụ n'ụwa nile. 'Shift' na-ezo aka na ngbanwe nke nje influenza A, na-ebute mpụta nke nje 'subtype' ọhụrụ. A na-ekewa nje virus influenza B ụzọ abụọ, nje Yamagata na Victoria Influenza B nwere naanị ihe mgbochi antigenic, ọ na-agbanarị nleba anya na mkpochapụ nke usoro ahụ mmadụ site na ngbanwe ya. Otú ọ dị, evolushọn ọsọ nke nje influenza B dị nwayọọ karịa nke nje influenza A mmadụ. Nje virus influenza B nwekwara ike ibute ọrịa iku ume mmadụ ma bute ọrịa na-efe efe.
Nje virus syncytial iku ume (RSV) bụ nje RNA, nke ezinụlọ paramyxoviridae. A na-ebunye ya site na ụmụ irighiri mmiri ikuku na nso nso a na ọ bụ isi ihe na-akpata ọrịa iku ume n'ime ụmụ ọhụrụ. Ụmụaka nwere ọrịa RSV nwere ike ịmalite bronchiolitis siri ike na oyi baa, nke metụtara ụkwara ume ọkụ na ụmụaka. Ụmụaka na-enwe mgbaàmà siri ike, gụnyere nnukwu ahụ ọkụ, rhinitis, pharyngitis na laryngitis, na mgbe ahụ bronchiolitis na oyi baa. Ụmụaka ole na ole na-arịa ọrịa nwere ike ịgbagwoju anya na mgbasa ozi otitis, pleurisy na myocarditis, wdg. Ọrịa nke akụkụ okuku ume elu bụ isi ihe mgbaàmà nke ọrịa na ndị okenye na ụmụaka ndị toro eto.
Nka nka
| Nchekwa | -18 ℃ N'ọchịchịrị |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | Oropharyngeal swab; Nasopharyngeal swab |
| Ct | IFV A, IFVB, RSV, SARS-CoV-2, IFV A H1N1Ct≤38 |
| CV | ≤5% |
| LoD | 200 oyiri/μL |
| Nkọwapụta | Nsonaazụ mmeghachi omume na-egosi na ọ nweghị mmeghachi omume n'etiti kit na cytomegalovirus, ọrịa herpes simplex ụdị 1, nje varicella zoster, nje Epstein-Barr, adenovirus, metapneumovirus mmadụ, rhinovirus, ụdị nje parainfluenza I/II/III/IV, bocavirus, enterovirus, coronavirus, Chhrumyelamonia p. Bordetella pertussis, Corynebacterium spp., Escherichia coli, Haemophilus influenzae, Lactobacillus spp., Legionella pneumophila, Moraxella catarrhalis, attenuated nsogbu nke Mycobacterium ụkwara nta, Neisseria meningitidis, Neisseria spp., Staphyloginoudosamon, Neisseria spp. Streptococcus pneumoniae, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium fasciatum, Nocardia, Serratia marcespercoccus, Cryptoscentr. fumigatus, Aspergillus flavus, Pneumocystis carinii, Candida albicans, Roseburia mucosa, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia Q fever na mmadụ genomic nucleic acid. |
| Ngwa ndị ọdabara | Ngwa Ngwa Ngwa Ngwa Ngwa Ngwa Ngwa PCR 7500 Ngwa Ngwa Ngwa Ngwa Ngwa Ngwa Ngwa Ngwa Ngwa Ngwa PCR 7500 Ngwa ngwa PCR, QuantStudio®Sistemụ PCR 5 ezigbo oge, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR Sistemu, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, Real-Time PCR System, PCR-Time PCR System, Real-Time PCR System. |
Usoro ọrụ
Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), na Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017-8 nwere ike iji E).TM AIO800 (HWTS-EQ007)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ihe nlele nke ewepụtara bụ 200μL yana olu elution akwadoro bụ 150μL.
