Ihe Ndị Na-akpata Ọrịa Iku Oku Ejikọtara
Aha ngwaahịa
Ngwa Nchọpụta Njikọta nke HWTS-RT183-Pathogens Respiratory (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa Korona nke afọ 2019, nke a na-akpọ 'COVID-19', na-ezo aka na oyi baa nke ọrịa SARS-CoV-2 kpatara. SARS-CoV-2 bụ coronavirus nke sitere na β genus. COVID-19 bụ ọrịa na-efe efe nke na-efe efe nke na-efe efe nke ukwuu, ndị mmadụ na-enwekarị ike ibute ya. Ugbu a, isi iyi nke ọrịa bụ ndị ọrịa butere na 2019-nCoV, ndị nwere ọrịa na-enweghị ihe mgbaàmà nwekwara ike ịghọ isi iyi nke ọrịa ahụ. Dabere na nyocha ọrịa na-efe efe ugbu a, oge mmalite nke ọrịa ahụ bụ ụbọchị 1-14, ọkachasị ụbọchị 3-7. Ahụ ọkụ, ụkwara kpọrọ nkụ na ike ọgwụgwụ bụ ihe ndị bụ isi na-egosi. Ụfọdụ ndị ọrịa nwere ihe mgbaàmà dịka mkpọchi imi, imi mmiri, akpịrị mgbu, myalgia na afọ ọsịsa, wdg. Influenza, nke a na-akpọkarị 'flu', bụ ọrịa na-efe efe nke nje influenza kpatara. Ọ na-efe efe nke ukwuu. A na-ebutekarị ya site na ụkwara na ịsụ ude. Ọ na-agbasakarị n'oge opupu ihe ubi na oyi. A na-ekewa nje influenza n'ime ụdị influenza A (IFV A), influenza B (IFV B), na influenza C (IFV C) atọ, ha niile bụ nje na-arapara arapara, na-akpata ọrịa mmadụ karịsịa maka nje influenza A na B, ọ bụ nje RNA nke nwere otu eriri, nke kewara ekewa. Nje influenza A bụ ọrịa iku ume dị oke njọ, gụnyere H1N1, H3N2 na ụdị ndị ọzọ, nke nwere ike ibute mgbanwe na mgbasa n'ụwa niile. 'Shift' na-ezo aka na mgbanwe nke nje influenza A, nke na-ebute mpụta nke 'ụdị' nje ọhụrụ. A na-ekewa nje influenza B n'ime usoro abụọ, nje Yamagata na Victoria Influenza B nwere naanị antigenic drift, ọ na-ezere nlekota na iwepụ usoro ahụ ji alụso ọrịa ọgụ mmadụ site na mgbanwe ya. Agbanyeghị, ọsọ evolushọn nke nje influenza B dị nwayọ karịa nke nje influenza A mmadụ. Nje influenza B nwekwara ike ibute ọrịa iku ume mmadụ ma bute ọrịa.
Nje virus nke na-eme ka mmadụ ghara iku ume (RSV) bụ nje RNA, nke sitere n'ezinụlọ paramyxoviridae. A na-ebufe ya site na ntapụ ikuku na kọntaktị chiri anya, ọ bụkwa isi ihe na-akpata ọrịa akụkụ iku ume ala n'ime ụmụ ọhụrụ. Ụmụ ọhụrụ nwere RSV nwere ike ibute nnukwu bronchiolitis na pneumonia, nke metụtara ụkwara ume ọkụ n'ime ụmụaka. Ụmụ ọhụrụ nwere nnukwu mgbaàmà, gụnyere oke ahụ ọkụ, rhinitis, pharyngitis na laryngitis, wee bụrụ bronchiolitis na pneumonia. Ụmụaka ole na ole na-arịa ọrịa nwere ike inwe mgbagwoju anya na otitis media, pleurisy na myocarditis, wdg. Ọrịa akụkụ iku ume elu bụ isi ihe mgbaàmà nke ọrịa n'ime ndị okenye na ụmụaka toro eto.
Usoro Nkà na ụzụ
| Nchekwa | -18℃ N'ọchịchịrị |
| Ndụ nchekwa | Ọnwa itoolu |
| Ụdị ihe atụ | Swab nke imi; Swab nke imi |
| Ct | IFV A, IFVB, RSV, SARS-CoV-2, IFV A H1N1Ct≤38 |
| CV | ≤5% |
| LoD | Mbipụta 200/mL |
| Nkọwapụta | Nsonaazụ mmeghachi omume na-egosi na enweghị mmeghachi omume n'etiti kit na cytomegalovirus, ụdị nje herpes simplex 1, nje varicella zoster, nje Epstein-Barr, adenovirus, nje metapneumovirus mmadụ, rhinovirus, ụdị nje parainfluenza I/II/III/IV, bocavirus, enterovirus, coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Bordetella pertussis, Corynebacterium spp., Escherichia coli, Haemophilus influenzae, Lactobacillus spp., Legionella pneumophila, Moraxella catarrhalis, ụdị nje Mycobacterium tuberculosis attenuated, Neisseria meningitidis, Neisseria spp., Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivary, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium fasciatum, Nocardia, Serratia marcescens, Citrobacter rodentium, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis carinii, Candida albicans, Roseburia mucosa, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia Q fever na human genomic nucleic acid. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR nke Ejiri Ngwa 7500, Sistemụ PCR nke Ejiri Ngwa 7500, QuantStudio®Sistemụ PCR ise n'oge a na-arụ ọrụ, Sistemụ PCR SLAN-96P n'oge a na-arụ ọrụ (Hongshi Medical Technology Co., Ltd.), LightCycler®Sistemụ PCR nke oge 480, LineGene 9600 Plus Sistemụ Nchọpụta oge PCR nke oge (FQD-96A, teknụzụ Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR Sistemụ, BioRad CFX Opus 96 Real-Time PCR Sistemụ. |
Usoro Ọrụ
Ngwa Ngwuputa DNA/RNA nke Macro na Micro-Test Viral (HWTS-3017) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), na Ngwa Ngwuputa DNA/RNA nke Macro & Micro-Test Viral (HWTS-3017-8) (nke enwere ike iji ya na Eudemon)TM AIO800 (HWTS-EQ007)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Olu ihe nlele ewepụtara bụ 200μL, olu ihe mgbapụta akwadoro bụ 150μL.
