SARS-CoV-2/influenza A/influenza B

Nkọwa Dị Mkpirikpi:

Ngwa a dabara adaba maka nchọpụta in vitro nke SARS-CoV-2, influenza A na influenza B nucleic acid nke nasopharyngeal swab na oropharyngeal swab samples nke ndị a na-enyo enyo na ha bu SARS-CoV-2, influenza A na influenza B. Enwere ike iji ya mee ihe na ndị a na-enyo enyo na ha bu ọrịa SARS-CoV-2, influenza A na influenza B. Enwere ike iji ya mee ihe na ndị a na-enyo enyo na ha bu ọrịa pneumonia na ndị a na-enyo enyo na maka nchọpụta qualitative na ịchọpụta SARS-CoV-2, influenza A na influenza B nucleic acid na nasopharyngeal swab na oropharyngeal swab samples nke ọrịa Coronavirus ọhụrụ n'ọnọdụ ndị ọzọ.

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Nkọwa Ngwaahịa

Akara Ngwaahịa

Aha ngwaahịa

Ngwa Nchọpụta Agwakọta nke HWTS-RT148-SARS-CoV-2/influenza A /influenza B Nucleic Acid (Fluorescence PCR)

Ọwa

Aha Ọwa	Ngwakọta PCR-1	PCR-Mix 2
Ọwa FAM	Mkpụrụ ndụ ihe nketa ORF1ab	IVA
Ọwa VIC/HEX	Njikwa dị n'ime	Njikwa dị n'ime
Ọwa CY5	Mkpụrụ ndụ ihe nketa N	/
Ọwa ROX	Mkpụrụ ndụ ihe nketa E	IVB

Usoro Nkà na ụzụ

Nchekwa	-18℃
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ihe atụ	swabs na nasopharyngeal na swabs oropharyngeal
Ebumnuche	Ihe mgbaru ọsọ atọ SARS-CoV-2 (mkpụrụ ndụ ihe nketa Orf1ab, N na E)/influenza A /influenza B
Ct	≤38
CV	≤10.0%
LoD	SARS-CoV-2：Mpempe 300/mL Ọrịa influenza A: 500 Mpempe akwụkwọ/mL Ọrịa influenza B: 500 Mpịakọta/mL
Nkọwapụta	a) Nsonaazụ nnwale ahụ gosiri na ngwa ahụ dakọtara na nje virus SARSr-CoV nke mmadụ, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, nje virus syncytial respiratory A na B, nje parainfluenza 1, 2 na 3, rhinovirusA, B na C, adenovirus 1, 2, 3, 4, 5, 7 na 55, nje metapneumovirus nke mmadụ, enterovirus A, B, C na D, nje virus pulmonary cytoplasmic nke mmadụ, nje EB, nje virus measles. nje virus cytomegalovirus nke mmadụ, rotavirus, norovirus, nje mumps, nje varicella zoster, nje mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata Enweghị mmeghachi omume n'etiti Pneumocystis yersini na Cryptococcus neoformans. b) Ike mgbochi nnyonye anya: select mucin (60mg/mL), 10% (V/V) ọbara mmadụ, diphenylephrine (2mg/mL), hydroxymethylzoline (2mg/mL), sodium chloride (nwere ihe nchekwa) (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisone (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), α-Interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), pramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceprotene (40μg/mL) Meropenem (200mg/mL), levofloxacin (10μg/mL) na tobramycin (0.6mg/mL). Nsonaazụ gosiri na ihe ndị na-egbochi ọrịa na njupụta dị n'elu enweghị nzaghachi ọ bụla na nsonaazụ nchọpụta nke nje.
Ngwa Ndị Dị Mkpa	Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa SLAN^®Sistemụ PCR oge-oge -96P Sistemụ PCR QuantStudio™ 5 n'oge a LightCycler^®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96