SARS-CoV-2/influenza A/influenza B

Nkọwa dị mkpirikpi:

Nke a kit bụ adabara in vitro qualitative nchọpụta nke SARS-CoV-2, influenza A na influenza B nucleic acid nke nasopharyngeal swab na oropharyngeal swab samples nke ndị mmadụ na-enyo enyo na ọ bụ ọrịa nke SARS-CoV-2, influenza A na influenza B. Ọ nwekwara ike iji na-enyo enyo na pflumoniit ikpe na p. mata SARS-CoV-2, influenza A na influenza B nucleic acid na nasopharyngeal swab na oropharyngeal swab sample nke novel Coronavirus ọrịa n'ọnọdụ ndị ọzọ.

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Nkọwa ngwaahịa

Mkpado ngwaahịa

Aha ngwaahịa

HWTS-RT148-SARS-CoV-2/influenza A/influenza B Nucleic Acid Ngwa Nchọpụta Ngwa (Fluorescence PCR)

Ọwa

Aha ọwa	PCR-Mix 1	PCR-Mix 2
Ọwa FAM	ORF1ab mkpụrụ ndụ ihe nketa	IVA
Ọwa VIC/HEX	Njikwa ime	Njikwa ime
Ọwa CY5	N gene	/
Ọwa ROX	E gene	IVB

Nka nka

Nchekwa	-18 ℃
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ụdị	nasopharyngeal swabs na oropharyngeal swabs
Ebumnuche	SARS-CoV-2 lekwasịrị anya atọ (Orf1ab, N na E mkpụrụ ndụ) / influenza A / influenza B
Ct	≤38
CV	≤10.0%
LoD	SARS-CoV-2: 300 oyiri/ml nje influenza A: 500 Copy/ml nje influenza B: 500 oyiri/ml
Nkọwapụta	a) The cross test results gosiri na ngwa ahụ dakọtara na mmadụ coronavirus SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, respiratory syncytial virus A na B, parainfluenza virus 1, 2 na 3, rhinovirus, 3, 3, 3, 3, 3, rhinovirus, ade2virus, rhinovirus, 3, 3, 3, 3, 3, 3, rhinovirus, ade2, rhinovirus. 5, 7 na 55, mmadụ metapneumovirus, enterovirus A, B, C na D, mmadụ cytoplasmic pulmonary virus, EB virus, measles virus Human cytomegalovirus, rotavirus, norovirus, mumps virus, varicella zoster virus, Mycoplasma pneumoniae, Chlamydia, pneumonia, Lemun tsami, Lemun tsami, Lemun tsami, Lemun tsami, Lemun tsami, Lemun tsami, Lemun tsami. influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium ụkwara nta, Aspergillus fumigatus, Candida albicans, Candida glabrata Enweghị mmeghachi omume cross n'etiti Pneumocystis yersinioform na Cryptoans. b) Mgbochi nnyonye anya ikike: họrọ mucin (60mg/ml), 10% (V/V) ọbara mmadụ, diphenylephrine (2mg/mL), hydroxymethylzoline (2mg/mL), sodium chloride (nwere preservative) (20mg/ml), beclomethasone (20mg/mL), dexamethasm2 (20mg/mL0), flunisone (20 mg / ml), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), α-Interferon (800IU/ml), zanamivir (20mg/mL), ribavirin (10mg/mL), ribavirin (10mg/mL). (1 mg / ml), lopinavir (500mg / ml), ritonavir (60mg / ml), mupirocin (20mg / ml), azithromycin (1mg / ml), ceprotene (40μg / ml) Meropenem (200mg / ml), levofloxacin (10.6 tom / L) Nsonaazụ gosiri na ihe ndị na-etinye aka na ntinye aka n'elu enweghị nzaghachi nnyonye anya na nsonaazụ nchọpụta nke ọrịa.
Ngwa ndị ọdabara	Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo SLAN^®-96P Real-Time PCR Systems QuantStudio™ 5 Sistemụ PCR ezigbo oge LightCycler^®480 ezigbo oge PCR usoro Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge