SARS-CoV-2/influenza A/influenza B
Aha ngwaahịa
HWTS-RT148-SARS-CoV-2/influenza A/influenza B Nucleic Acid Ngwa Nchọpụta Ngwa (Fluorescence PCR)
Ọwa
Aha ọwa | PCR-Mix 1 | PCR-Mix 2 |
Ọwa FAM | ORF1ab mkpụrụ ndụ ihe nketa | IVA |
Ọwa VIC/HEX | Njikwa ime | Njikwa ime |
Ọwa CY5 | N gene | / |
Ọwa ROX | E gene | IVB |
Nka nka
Nchekwa | -18 ℃ |
Ndụ nchekwa | Ọnwa iri na abụọ |
Ụdị ụdị | nasopharyngeal swabs na oropharyngeal swabs |
Ebumnuche | SARS-CoV-2 lekwasịrị anya atọ (Orf1ab, N na E mkpụrụ ndụ) / influenza A / influenza B |
Ct | ≤38 |
CV | ≤10.0% |
LoD | SARS-CoV-2: 300 oyiri/ml nje influenza A: 500 Copy/ml nje influenza B: 500 oyiri/ml |
Nkọwapụta | a) Nsonaazụ ule obe gosiri na ngwa ahụ dakọtara na coronavirus mmadụ SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, nje syncytial respiratory A na B, nje parainfluenza 1, 2 na 3, rhinovirusA, B na C, adenovirus 1, 2, 3, 4, 5, 7 na 55, mmadụ metapneumovirus, enterovirus A, B, C na D, mmadụ cytoplasmic pulmonary virus, EB virus, measles virus Human cytomegalovirus, rotavirus, norovirus, nje mumps, varicella zoster virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pneumoniae, Streptococcus pneumoniae, mygacoccus pneumonia, mygacoccus pneumonia. Candida albicans, Candida glabrata Ọ dịghị mmeghachi omume n'etiti Pneumocystis yersini na Cryptococcus neoformans. b) Ikike mgbochi mgbochi: họrọ mucin (60mg/mL), 10% (V/V) ọbara mmadụ, diphenylephrine (2mg/mL), hydroxymethylzoline (2mg/mL), sodium chloride (nwere preservative) (20mg/mL), beclomethasone (20mg / ml), dexamethasone (20mg / ml), flunisone (20μg / ml), triamcinolone acetonide (2mg / ml), budesonide (2mg / ml), mometasone (2mg / ml), fluticasone (2mg / mL), histamine hydrochloride (5mg/mL), α-Interferon (800IU/ml), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/ml), pramivir (1mg/mL), lopinavir (500mg/mL). Ritonavir (60mg / ml), mupirocin (20mg / ml), azithromycin (1mg / ml), ceprotene (40μg / ml) Meropenem (200mg / ml), levofloxacin (10μg / ml) na tobramycin (0.6mg/mL). .Nsonaazụ gosiri na ihe ndị na-etinye aka na ntinye aka n'elu enweghị nzaghachi nnyonye anya na nsonaazụ nchọpụta nke ọrịa. |
Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo SLAN ®-96P Real-Time PCR Systems QuantStudio™ 5 Sistemụ PCR ezigbo oge LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |