Ngwa Nchọpụta Acid Nucleic nke Staphylococcus Aureus na Methicillin nke Na-eguzogide Staphylococcus Aureus
Aha ngwaahịa
Ngwa Nchọpụta Acid Nucleic nke Staphylococcus Aureus na Methicillin (Fluorescence PCR)
Ojiji Ezubere
A na-eji ngwa a maka ịchọpụta staphylococcus aureus na staphylococcus aureus nucleic acids nke na-eguzogide methicillin na ihe nlele sputum mmadụ, ihe nlele imi na ihe nlele ọrịa akpụkpọ ahụ na anụ ahụ dị nro na vitro. A na-eji ihe nlele sputum na ihe nlele imi swab na ụlọ ọgwụ iji gbochie ma chịkwaa ọrịa MRSA nosocomial na ndị ọrịa nọ n'ụlọ ọgwụ, gụnyere ndị ọrịa nlekọta siri ike, ndị ọrịa ịwa ahụ na ndị ọrịa nlekọta ogologo oge; A pụkwara iji ya maka nchọpụta enyemaka nke ndị a na-enyo enyo na ha bụ Staphylococcus Aureus na ndị ọrịa Methicillin-Resistant Staphylococcus Aureus na-ebute ọrịa iku ume; Ma ọ bụ yana nyocha ụlọ nyocha ndị ọzọ dị ka ọdịbendị nje iji nyere aka na nchọpụta nke ọrịa akpụkpọ ahụ na anụ ahụ dị nro MRSA/SA. A naghị eji nsonaazụ ule ozugbo eduzi ojiji ọgwụ nje.
Ọmụmụ ihe gbasara ọrịa na-efe efe
Staphylococcus aureus bụ otu n'ime nje bacteria dị mkpa nke na-akpata ọrịa nosocomial. Staphylococcus aureus (SA) bụ nke staphylococcus ma bụrụkwa onye nnọchite anya nje bacteria Gram-positive, nke nwere ike imepụta ọtụtụ nsí na enzymes ndị na-awakpo ahụ. Nje bacteria ahụ nwere njirimara nke mgbasa sara mbara, ọrịa siri ike na ọnụego iguzogide dị elu. Mkpụrụ ndụ ihe nketa nuclease nke a na-akpọ Thermostable (nuc) bụ mkpụrụ ndụ ihe nketa staphylococcus aureus nke a na-echekwa nke ọma.
N'afọ ndị na-adịbeghị anya, n'ihi ojiji nke homonụ na nkwadebe mgbochi ọrịa na iji ọgwụ nje mee ihe nke ukwuu, ọrịa nje ndị na-anaghị alụso ọrịa ọgụ nke Methicillin na-akpata na Staphylococcus aureus (MRSA) na Staphylococcus amụbaala. Ọnụego nchọpụta MRSA nke mba ahụ bụ 30.2% na 2019 na China. A na-ekewa MRSA n'ime MRSA metụtara ahụike (HA-MRSA), MRSA metụtara obodo (CA-MRSA), na MRSA metụtara anụ ụlọ (LA-MRSA). CA-MRSA, HA-MRSA, LA-MRSA nwere nnukwu ọdịiche na microbiology, iguzogide nje bacteria (dịka ọmụmaatụ, HA-MRSA na-egosi iguzogide ọtụtụ ọgwụ karịa CA-MRSA) na njirimara ahụike (dịka ọmụmaatụ ebe ọrịa ahụ dị). Dịka njirimara ndị a si dị, enwere ike ịmata ọdịiche dị n'etiti CA-MRSA na HA-MRSA. Agbanyeghị, ọdịiche dị n'etiti CA-MRSA na HA-MRSA na-ebelata n'ihi mmegharị mgbe niile nke ndị mmadụ n'etiti ụlọ ọgwụ na obodo. MRSA na-eguzogide ọtụtụ ọgwụ, ọ bụghị naanị na ọ na-eguzogide ọgwụ nje β-lactam, kamakwa ọ na-eguzogide aminoglycosides, macrolides, tetracyclines na quinolones n'ọtụtụ ọkwa dị iche iche. E nwere nnukwu ọdịiche mpaghara na ọnụego iguzogide ọgwụ na usoro dị iche iche. MRSA nwere ike ịbanye n'ahụ site na mmerụ ahụ akpụkpọ ahụ, follicles ntutu, ọbara na ihe ndị ọzọ na-akpata ọrịa suppurative, ndị ọrịa nwere ọrịa akpụkpọ ahụ na ọkụ na-enwekwa oke ike ibute ọrịa MRSA. Pneumonia bụ otu n'ime ọrịa ndị a na-ahụkarị na MRSA, ọgwụgwọ na-ekwesịghị ekwesị bụkwa ihe dị mkpa na-akpata ọnwụ dị elu.
Usoro Nkà na ụzụ
| Nchekwa | ≤ -18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | Sputum, imi swab, akpụkpọ ahụ na anụ ahụ dị nro sample ọrịa, |
| CV | <5.0% |
| LoD | 1000 CFU/mL |
| Nkọwapụta | Nnwale mmeghachi omume nke cross-reactivity na-egosi na ngwa a enweghị mmeghachi omume cross-reactivity na nje ndị ọzọ na-efe efe dịka methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Ngwa Ndị Dị Mkpa | Ụdị nke Mbụ: Sistemụ PCR nke Biosystems 7500 Real-Time, Sistemụ PCR nke QuantStudio®5 Real-Time, Sistemụ PCR nke SLAN-96P Real-Time (Hongshi Medical Technology Co., Ltd.), Sistemụ Nchọpụta PCR nke LineGene 9600 Plus Real-Time (FQD-96A, teknụzụ Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Sistemụ PCR nke BioRad CFX96 Real-Time, Sistemụ PCR nke BioRad CFX Opus 96 Real-Time. Ụdị nke Abụọ: HWTS-EudemonTM AIO800 |
Usoro Ọrụ
Ihe ndị na-eme ka ihe ... ghara ịdị mkpa bụ ihe ndị a na-eme ka ihe na-eme ka ihe
Ụdị m
(1) Ngwa Ngwuputa DNA/RNA nke Ngwa ...
(2) Ihe nlele ihe ọṅụṅụ: 4% NaOH ma ọ bụ Sputasol sitere na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. (STXB 20220253)
(3) Nnu mmiri nkịtị.
Ụdị nke Abụọ:
Ngwa DNA/RNA izugbe nke Macro & Micro-Nnwale (HWTS-3019-8) sitere na Jiangsu Macro & Micro-Nnwale Med-Tech Co., Ltd.
A chọrọ ihe oriri mana enyeghị ha:Ntuziaka ndị na-enweghị RNase/DNase, uwe aka a na-atụfu atụfu, tube EP ndị na-enweghị RNase/DNase, mpempe tube 8 maka PCR.
