Staphylococcus Aureus na Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)
Aha ngwaahịa
Ngwa Nchọpụta Acid Nucleic nke HWTS-OT062 Staphylococcus Aureus na Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) (Fluorescence PCR)
Asambodo
CE
Ọmụmụ ihe gbasara ọrịa na-efe efe
Staphylococcus aureus bụ otu n'ime nje bacteria dị mkpa nke na-akpata ọrịa nosocomial. Staphylococcus aureus (SA) bụ nke staphylococcus ma bụrụkwa onye nnọchite anya nje bacteria Gram-positive, nke nwere ike imepụta ọtụtụ nsí na enzymes ndị na-awakpo ahụ. Nje bacteria ahụ nwere njirimara nke mgbasa sara mbara, ọrịa siri ike na ọnụego iguzogide dị elu. Mkpụrụ ndụ ihe nketa nuclease nke a na-akpọ Thermostable (nuc) bụ mkpụrụ ndụ ihe nketa staphylococcus aureus nke a na-echekwa nke ọma.
Ọwa
| FAM | Mkpụrụ ndụ ihe nketa mecA na-eguzogide methicillin |
| ROX | Njikwa Dị n'ime |
| CY5 | mkpụrụ ndụ ihe nketa staphylococcus aureus |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ & echekwara ya site na ìhè |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | ihe nlele ọrịa afụ ọnụ, ihe nlele ọrịa akpụkpọ ahụ na anụ ahụ dị nro, yana ihe nlele imi swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, nje bacteria na-eguzogide methicillin 1000 CFU/mL. Mgbe ngwa ahụ chọpụtara ntụaka mba LoD, enwere ike ịchọpụta staphylococcus aureus 1000/mL |
| Nkọwapụta | Nnwale mmeghachi omume nke cross-reactivity na-egosi na ngwa a enweghị mmeghachi omume cross-reactivity na nje ndị ọzọ na-efe efe dịka methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P Oge-N'ezie LightCycler®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Nhọrọ nke 1.
Enwere ike iji Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. mee ihe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Tinye 200µL nke saline nkịtị na ihe e ji eme ihe, a ga-ewepụtakwa usoro ndị na-esote dịka ntuziaka si dị, olu ihe e ji eme ihe a tụrụ aro bụ 80µL.
Nhọrọ nke abụọ.
Ngwa Mwepụta Ihe Nlereanya Macro & Micro-Test (HWTS-3005-8) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tinye 1mL nke saline nkịtị na ihe mkpuchi ahụ mgbe ị sachara ya na saline nkịtị, wee gwakọta ya nke ọma. Na-eme ka ọ dị n'ime 13,000r/min maka nkeji ise, wepụ ihe mkpuchi ahụ (dobe 10-20µL nke ihe mkpuchi ahụ), ma soro ntuziaka maka mwepụta ya mgbe e mesịrị.
Ihe na-eme ka a na-ewepụta ihe n'ime mmiri: Ngwuputa Acid Nucleic ma ọ bụ Nchacha Reagent (YDP302) nke Tiangen Biotech (Beijing) Co.,Ltd. A ga-eme mwepụ ahụ kpọmkwem dịka nzọụkwụ nke abụọ nke akwụkwọ ntuziaka si dị. A na-atụ aro ka e jiri mmiri RNase na DNase na-enweghị mmiri mee ka ọ dị mmiri mmiri nke 100µL.
