Staphylococcus Aureus na Methicillin na-eguzogide Staphylococcus Aureus (MRSA/SA)
Aha ngwaahịa
HWTS-OT062 Staphylococcus Aureus na Methicillin na-eguzogide Staphylococcus Aureus (MRSA/SA) Ngwa nchọpụta Nucleic Acid (Fluorescence PCR)
Asambodo
CE
Epidemiology
Staphylococcus aureus bụ otu n'ime nje bacteria na-akpata ọrịa nosocomial.Staphylococcus aureus (SA) bụ nke staphylococcus ma bụrụ onye nnọchianya nke nje bacteria Gram-positive, nke nwere ike ịmepụta nsị dị iche iche na enzymes na-emerụ ahụ.Nje bacteria nwere njirimara nke nkesa sara mbara, pathogenicity siri ike na oke nguzogide.Mkpụrụ ndụ ihe nketa nuclease (nuc) bụ mkpụrụ ndụ ihe nketa nke staphylococcus aureus echekwara nke ukwuu.
Ọwa
| FAM | gene mecA na-eguzogide methicillin |
| ROX | Njikwa ime |
| CY5 | staphylococcus aureus nuc gene |
Nka nka
| Nchekwa | ≤-18℃ & echebe site na ìhè |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | sputum, akpụkpọ ahụ na anụ ahụ dị nro ọrịa sample, na imi imi |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/ml staphylococcus aureus, 1000 CFU/mL nje bacteria na-eguzogide methicillin.Mgbe ngwa ahụ chọpụtara ntụnye aka LoD nke mba, enwere ike ịhụ 1000/mL staphylococcus aureus |
| Nkọwapụta | Nnwale nlegharị anya nke cross-reactivity na-egosi na ngwa a enweghị ihe ọ bụla na-emeghachi omume na nje ndị ọzọ na-eku ume ume dị ka methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, staphylococcus epidermidis na-eguzogide methicillin, pseudomonas aeruginosa, escherichia coli, kleetociniia, pseudomonas aeruginosa. Ndị na-ahụ maka ọrịa strok, streptococcus pneumonia, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, neisseria meningitidis, haemophilus influenzae. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Sistemu QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1.
Enwere ike iji Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. na-eji Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Tinye 200µL nke saline nkịtị n'ime mmiri a na-ahazi, yana usoro ndị na-esote kwesịrị ịwepụta dịka ntuziaka ahụ si dị, na olu elution akwadoro bụ 80µL.
Nhọrọ 2.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tinye 1mL nke saline nkịtị ka ọ na-asọpụta mgbe ịsachara ya na saline nkịtị, wee gwakọta nke ọma.Centrifuge na 13,000r/min maka nkeji 5, wepụ ihe dị elu (chekwaa 10-20µL nke supernatant), ma soro ntuziaka maka mmịpụta na-esote.
Reagent mmịpụta akwadoro: Nucleic Acid Extraction or Purification Reagent (YDP302) nke Tiangen Biotech (Beijing) Co., Ltd.Ekwesịrị ịme mmịpụta ahụ nke ọma dịka nzọụkwụ 2 nke ntuziaka ntuziaka si dị.A na-atụ aro ka iji RNase na mmiri na-enweghị DNA maka elution na oke 100µL.
