Staphylococcus aureus na methillin-eguzogide staphylococs auus (MRSA / SA)
Aha
HWT-OT062 staphyillin-na-eguzogide staphylococs aureus (MRSA / S) nucleic acid Kit (Fluorescence Pc)
Asambodo
CE
Mpidermiology
Staphylococcus aureus bụ otu n'ime nje bacteria dị mkpa nke ọrịa imicomomial. Staphylococcus aureus (s) bụ nke staphylococcus ma bụrụ onye nnọchi anya nje dị mma, nke nwere ike iwepụta ụdị nsị na enzymes dị iche iche. Ndị nje bacteria ahụ nwere njirimara nke nkesa dị obosara, ọrịa siri ike na nguzozi dị elu. Igwe ikuku nuclease Gene (nuc) bu udiri udiri nke staphylococus aure.
Chaneelu
M | Methicillin-na-eguzogide maca |
Rox | Nchịkwa ime |
Pur5 | staphylococcus auus nuc Gene |
Nrụta Nka na ụzụ
Storaji | ≤-18 ℃ & chebere site na ọkụ |
Ebe ndu | 12 ọnwa |
Ụdị ụdị | sputum, a na-ahụ maka anụ ahụ nke anụ ahụ, naSal swab |
Ct | ≤36 |
CV | ≤5.0% |
Loo | 1000 cfu / ml staphylococcus aureus, 1000 cfu / ml methicillin-na-eguzogide ọgwụ. Mgbe ndị Kit na-achọpụta ihe na-egosi na mba Lodo, 1000 / ML Staphyloccoluccus a nwere ike ịchọpụta |
Nnoshi | Esemokwu na-eme ihe na-egosi na nke a na-enweghị ike na-ekwupụta na ndị ọzọ na-efe efe na ndị ọzọ na-efe efe, na-eguzogide staphylocosa, na-eguzogide perli, klebiliella na Ugbu a, onye na-atụ anya Mirabilis, Straptrococus pyoonia, onye na-ahụ maka facetium, certoonocs, cersionela pneumophila, ndị na-ahụ maka ihe nkiri, ndị na-ahụ maka ihe nkiri, hemophilus na-emetụta. |
Ngwá agha | Ngwa Biosystems na-etinye 7500 ezigbo oge pcr Nke quagruo®5 ezigbo oge pcr Slan-96p ezigbo oge pcr Lightcycler®480 ezigbo oge pcr sistemụ Linegene 9600 Pụnyere Real Chemical System Ma-6000 Real-Oge lyr Beired cfx96 ezigbo oge pcr sistemụ Beired cfx opus 96 Real System |
Na-arụ ọrụ
Nke 1.
Macro & Micro-ule DNA / RNA-3019) nke Jiangsu Macro & Micro-Tech Acid Acid Ngwá Ọrụ (HWD-3006C, HWS- 3006B). Tinye 200μL nke saline zuru oke na forumpiping, na usoro ndị sochiri a ga-ewepụta ya dị ka ndị na-atụ aro ya si dị, yana oke ugwọ a tụrụ aro bụ 80μl.
Nke 2.
Mpempe akwụkwọ Nleta Macro & Micro-Nnwale (HWTS-3005-8) site na Jiangsu Macro & Micro-Nnwale Med-Tech nke saline na-asacha ya na nnu dị mma, wee gwakọta nke ọma. Centrifuge na 13,000r / min maka nkeji 5, wepu supernant (reserve 10-20μL nke supernatint), ma soro ntuziaka maka mmịpụta ndị na-esote.
A na-atụ aro mmịpụta ntụgharị: Mwepu acid dị ọcha (YDP302 biotech (Beijing) Co., LTD. A ga-emepụta mmịpụta ya dabere na nzọụkwụ 2 nke akwụkwọ ntuziaka. A na-atụ aro ka iji mmiri na-akwụghị mma maka mkpali nke 100μl.