Treponema Pallidum Nucleic Acid
Aha ngwaahịa
HWTS-UR047-Treponema Pallidum Nucleic Acid Nchọpụta Ngwa (Fluorescence PCR)
Epidemiology
Syphilis bụ ọrịa a na-ebutekarị site na mmekọahụ na omume ụlọ ọgwụ, ọkachasị na-ezo aka na ọrịa na-adịghị ala ala, nke a na-ebute site na mmekọahụ nke Treponema Pallidum (TP) butere. Ọrịa syphilis na-ebutekarị site na mbufe mmekọahụ, ibufe nne na nwa na nnyefe ọbara. Ndị na-arịa ọrịa syphilis bụ nanị isi iyi nke ọrịa, na Treponema pallidum nwere ike ịdị na semen, mmiri ara ara, mmiri mmiri na ọbara. Enwere ike kewaa syphilis ụzọ atọ dịka usoro ọrịa si dị. Ọrịa syphilis nke ọkwa mbụ nwere ike ịpụta dị ka chancre siri ike na ọzịza nke lymph nodes inguinal, n'oge a kachasị na-efe efe. Ọrịa syphilis nke ọkwa nke abụọ nwere ike ịpụta dị ka ọkụ ọkụ syphilitic, chancre siri ike na-ebelata, ọrịa na-efe efe dịkwa ike. Syphilis nke ogbo nke atọ nwere ike igosipụta dị ka syphilis ọkpụkpụ, neurosyphilis, wdg.
Nka nka
| Nchekwa | -18 ℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | nwoke urethral swab, nwanyi cervical swab, nwanyi ikpu swab. |
| Ct | ≤38 |
| CV | ≤10.0% |
| LoD | 400 oyiri/μL |
| Ngwa ndị ọdabara | Ọdabara na ụdị I nchọpụta reagent: Ngwa Biosystem 7500 ezigbo oge PCR, QuantStudio®Sistemụ PCR 5 ezigbo oge, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, teknụzụ Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Sistemụ PCR nke BioRad CFX96, BioRad CFX Opus 96 PCR ezigbo oge. Ọdabara maka ụdị II reagent nchọpụta: EudemonTMAIO800 (HWTS-EQ007) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Usoro ọrụ
Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), na Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017-8 nwere ike iji E).TM AIO800 (HWTS-EQ007)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ihe nlele nke ewepụtara bụ 200μL yana olu elution akwadoro bụ 150μL.
