Xinjiang Hemorrhagic Fever Virus
Aha ngwaahịa
HWTS-FE007B/C Xinjiang Hemorrhagic Fever Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Ọrịa Xinjiang Hemorrhagic fever bụ nke mbụ kewapụrụ n'ọbara ndị ọrịa nwere ahụ ọkụ na-efe efe na Tarim Basin, Xinjiang, China na akọrọ siri ike ejidere na mpaghara, wee nweta aha ya. Clinical ngosipụta na-agụnye ahụ ọkụ, isi ọwụwa, ọbara ọgbụgba, hypotensive ujo, wdg The isi pathological mgbanwe nke ọrịa a bụ systemic capillary dilatation, congestion, ụba permeability na fragility, n'ihi na iche iche degrees nke mkpọkọ na hemorrhage na akpụkpọ na mucous membranes nakwa dị ka anụ ahụ nke dị iche iche akụkụ n'ime ahụ dum, na degeneration na necrosis nke a gland, imeju na akụkụ ahụ siri ike, dị ka akụkụ ahụ nke gland na akụkụ ahụ siri ike. jelii-dị ka edema na retroperitoneum.
Ọwa
| FAM | Xinjiang Hemorrhagic Fever Virus |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | ọbara ọhụrụ |
| Tt | ≤38 |
| CV | .5.0% |
| LoD | 1000 oyiri/ml |
| Nkọwapụta | Ọ dịghị cross-reactivity na ndị ọzọ akụkụ okuku ume samples dị ka Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneu/B1. nje syncytial A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, wdg na mmadụ genomic DNA. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR, Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®480 ezigbo oge PCR usoro Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (nke ike ga-eji na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) site Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The mmịpụta ga-regent na-eduzi dị ka nke a na mmịpụta. Olu nlele ewepụtara bụ 200µL, na olu elution akwadoro bụ 80µL.
Reagent mmịpụta akwadoro: QIAamp Viral RNA Mini Kit (52904) nke QIAGEN na Nucleic Acid Extraction ma ọ bụ Reagent Ọcha (YDP315-R). Ekwesịrị ịme mmịpụta ahụ n'ụzọ siri ike dabere na ntuziaka maka ojiji. Olu nlele ewepụtara bụ 140µL, na olu elution akwadoro bụ 60µL.
