Ọrịa Ahụ́ Ọbara nke Xinjiang
Aha ngwaahịa
Ngwa Nchọpụta Acid Nucleic nke HWTS-FE007B/C nke nje virus hemorrhagic fever nke Xinjiang (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
E bu ụzọ chọpụta nje Xinjiang hemorrhagic fever site n'ọbara ndị ọrịa nwere hemorrhagic fever na Tarim Basin, Xinjiang, China na akọrọ siri ike e jidere n'ógbè ahụ, wee nweta aha ya. Ihe mgbaàmà ahụike gụnyere ahụ ọkụ, isi ọwụwa, ọbara ọgbụgba, oke mkpọtụ ọbara mgbali elu, wdg. Mgbanwe ndị bụ isi nke ọrịa a bụ mgbasa nke capillary systemic, mkpọchi, mmụba nke permeability na adịghị ike, nke na-ebute oke mkpọchi na ọbara ọgbụgba dị iche iche na akpụkpọ ahụ na akpụkpọ ahụ mucous yana anụ ahụ nke akụkụ ahụ dị iche iche n'ahụ, yana mmebi na necrosis nke akụkụ ahụ siri ike dịka imeju, gland adrenal, pituitary gland, wdg, na edema dị ka jelly na retroperitoneum.
Ọwa
| FAM | Ọrịa Ahụ́ Ọbara nke Xinjiang |
| ROX | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa itoolu |
| Ụdị ihe atụ | serum ọhụrụ |
| Tt | ≤38 |
| CV | ＜5.0% |
| LoD | 1000Mpempe/mL |
| Nkọwapụta | Enweghị mmeghachi omume mmegharị ahụ na ihe nlele iku ume ndị ọzọ dịka Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneumovirus A1/A2/B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, wdg. na DNA genomic mmadụ. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500, Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P Oge-N'ezie LightCycler®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Ihe na-eme ka a na-ewepụta ihe: Ngwakọta DNA/RNA nke Macro & Micro-Test Viral (HWTS-3017) (nke enwere ike iji Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. A ga-eme mwepụta ahụ dịka ntuziaka maka ojiji nke ihe na-eme ka a na-ewepụta ihe a si dị. Olu ihe nlele ahụ ewepụtara bụ 200µL, olu mwepụta ahụ akwadoro bụ 80µL.
Ihe eji ewepụta ihe: QIAamp Viral RNA Mini Kit (52904) nke QIAGEN na Nucleic Acid Extraction or Purification Reagent (YDP315-R). A ga-eme mwepụ ahụ dịka ntuziaka maka ojiji si dị. Olu ihe nlele ewepụtara bụ 140µL, olu mwepụ ahụ akwadoro bụ 60µL.
