Ọrịa Ebola na Zaire
Aha ngwaahịa
Ngwa Nchọpụta Asịd Nucleic nke Nje Virus Ebola nke HWTS-FE008 (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Nje Ebola bụ nke Filoviridae, nke bụ nje RNA nke na-anaghị ekewa otu eriri. Nje virus bụ eriri ogologo nwere nkezi ogologo virion nke 1000nm na dayameta nke ihe dị ka 100nm. Nje virus Ebola bụ RNA nke na-anaghị ekewa nke nwere nha 18.9kb, nke na-akọwa protein nhazi 7 na otu protein na-abụghị nhazi. Enwere ike kewaa nje Ebola n'ụdị dịka Zaire, Sudan, Bundibugyo, Tai Forest na Reston. N'ime ha, akọpụtala na ụdị Zaire na ụdị Sudan na-akpata ọnwụ ọtụtụ mmadụ site na ọrịa. EHF (Ebola Hemorrhagic Fever) bụ ọrịa na-efe efe nke nje Ebola na-akpata. Ndị mmadụ na-ebutekarị ọrịa site na kọntaktị na mmiri ahụ, ihe ndị na-apụta na ihe ndị na-apụta n'ahụ, ihe ndị na-apụta na ihe ndị na-apụta n'ahụ ma ọ bụ anụmanụ ndị bu ọrịa, ihe ngosi ahụike bụkarị ahụ ọkụ, ọbara ọgbụgba na mmebi akụkụ ahụ dị iche iche. EHF nwere oke ọnwụ nke 50%-90%.
Ọwa
| FAM | MP nucleic acid |
| ROX | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa itoolu |
| Ụdị ihe atụ | Serọm ọhụrụ, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Mbipụta 500/μL |
| Nkọwapụta | Jiri ngwa ndị ahụ nwalee ntụaka ụlọ ọrụ na-adịghị mma, nsonaazụ ya na-emezu ihe achọrọ. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa Sistemụ PCR nke QuantStudio®5 n'oge dị adị Sistemụ PCR SLAN-96P nke Oge-N'ezie (Hongshi Medical Technology Co., Ltd.) Sistemụ PCR oge-oge LightCycler®480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya (Suzhou Molarray Co., Ltd.) Sistemụ PCR BioRad CFX96 Real-Time, na Sistemụ PCR BioRad CFX Opus 96 Real-Time |
Usoro Ọrụ
Nhọrọ nke 1.
Ihe eji ewepụta ihe a tụrụ aro: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) nke Tiangen Biotech (Beijing) Co., Ltd. A ga-ewepụta ya dịka ntuziaka si dị, olu mwepụta ihe nlele akwadoro bụ 140μL yana olu mwepụta akwadoro bụ 60μL.
Nhọrọ nke abụọ.
Ihe na-eme ka a na-ewepụta ihe ndị a tụrụ aro: Ngwa DNA/RNA nke Macro & Micro-Test Viral (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). A ga-ewepụta ya dịka ntuziaka si dị. Olu ihe nlele ahụ bụ 200μL, olu mgbapụta ahụ akwadoro bụ 80μL.
Nhọrọ nke 3.
Ihe ndị a tụrụ aro maka mwepụta: A ga-ewepụta ihe nnọchite anya mmepụta Nucleic Acid (1000020261) na sistemụ nkwadebe ihe nlele akpaaka nke High Throughput (MGISP-960) site na BGI dịka ntuziaka si dị. Olu mwepụta ahụ bụ 160μL, olu mwepụta akwadoro bụ 60μL.
