Zaire Ebola virus
Aha
HWT-Fe008 Zaire Ebola virus nucleic Acid Nchọpụta Kit (Fluorescence Pc)
Mpidermiology
Ọrịa Ebola nwe Floviridae, nke bụ nje na-adịghị mma HNA na-adịghị mma. Njeghari bu ogologo fileraments na nkezi vionle ogologo 1000nm na dayameta nke ihe dị ka 100nm. Nje Virus Eboola bụ ihe na-adịghị mma RNA nke na-adịghị mma nke 18.9KB, na-eme ka protein 7 na protein na-abụghị nke. Enwere ike kee nje Ebola dị ka Zaire, Sudan, Bundan Bundá, Tai ọhịa na Enton. N'ime ha, a kọọla ụdị Zire na ụdị ndị Sudan iji mee ọtụtụ ọnwụ nke mmadụ site na ọrịa. Ehf (Ebola Hemorrhagic ahụ) bụ ọrịa na-efe efe na-efe efe nke nje oria Ebola kpatara. A na - ebute ụmụ mmadụ site na ịkpọtụrụ mmiri ahụ, ebịọ na nke Excreta nke ndị ọrịa ma ọ bụ anụmanụ na - ebute ọrịa, na ngosipụta ọgbụgba na ọtụtụ anụ ahụ. Ehf nwere oke onwu nke 50% -90%.
Chaneelu
| M | Mp nucleic acid |
| Rox | Nchịkwa ime |
Nrụta Nka na ụzụ
| Storaji | ≤-18 ℃ |
| Ebe ndu | Ọnwa 9 |
| Ụdị ụdị | Ọhụrụ ọbara, plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| Loo | 500 mbipụta / μl |
| Nnoshi | Jiri Kits iji nwalee ụlọ ọrụ na-adịghị mma, nsonaazụ ahụ na-emezu ihe ndị achọrọ. |
| Ngwá agha | Ngwa Biosystems na-etinye 7500 ezigbo oge pcr Ngwa Biosystems na-etinye 7500 ngwa ngwa usoro pcr Systrudio®5 ezigbo oge pcr Oge Slan-96p Real-96p Systems (Hongshili Medical Teknụzụ CO., LTD.) Lightcycler®480 Real System System Linegene 9600 gbakwunyere ezigbo oge Pcr na-achọpụta usoro (FQD-96A, teknụzụ Bioner) Ma-6000 Real-Oge gwọike Cycler (Suzhou Molerray Co., LTD.) Beired CFX96 ezigbo oge Pcr sistemụ, na BIORAD CFX OPUS 96 Real System |
Na-arụ ọrụ
Nke 1.
A na-atụ aro mmịpụta ntụgharị: Qiap RAIL RA MINI Kit (52904) Ọ ga-amịpụta ya na ntuziaka, na ọnụego mmịpụta nke atụnyere dị 140μl na olu ure akwadoro bụ 60μL.
Nke 2.
A na-atụ aro mmịpụta ntụgharị: macro & Micro-ule (HWT-3004-34, HWT-3004-96) na-emepụta Acid Actocket (HWT-3006) .it ekwesịrị iwepụta ya dị ka ntuziaka si dị. Olu mmịpụta ahụ bụ 200μL, na olu ure akwadoro bụ 80μL.
Nke 3.
A na-atụ aro ndị na-emegharị akwụkwọ ntụgharị: Ntinye Acid Acid (1000020261) na elu site na nnukwu nkwadebe Sistem na-akpaghị aka (MGISP-96) site na ntuziaka. Olu mmịpụta bụ 160μL, na olu urọ a tụrụ aro ya bụ 60μL.
