Ọrịa Zika
Aha ngwaahịa
Ngwa Nchọpụta Asịd Nucleic nke Nje Virus Zika nke HWTS-FE002 (Fluorescence PCR)
Asambodo
CE
Ọmụmụ ihe gbasara ọrịa na-efe efe
Nje Zika so n'ime ụdị Flaviviridae, ọ bụ nje RNA nke nwere otu eriri positive-stranded nke nwere dayameta nke 40-70nm. O nwere envelopu, nwere nucleotides 10794, ma na-akọwapụta amino acid 3419. Dịka ụdị genotype si dị, a na-ekewa ya n'ụdị Afrịka na ụdị Eshia. Ọrịa nje Zika bụ ọrịa na-efe efe nke na-egbochi onwe ya nke nje Zika na-akpata, nke a na-ebutekarị site na ata anwụnta Aedes aegypti. Ihe ndị a na-ahụkarị bụ ahụ ọkụ, ihe ọkụ, ọrịa arthralgia ma ọ bụ conjunctivitis, ọ naghịkwa egbu egbu. Dịka Òtù Ahụ Ike Ụwa si kwuo, obere nwa ọhụrụ na ọrịa Guillain-Barre (Guillain-Barré syndrome) nwere ike ịdị na ya na ọrịa nje Zika.
Ọwa
| FAM | Ọrịa Zika nke nje nucleic acid |
| ROX | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤30℃ & echekwara ya site na ìhè |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | serum ọhụrụ |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500Mpempe/mL |
| Nkọwapụta | Jiri ngwa ahụ chọpụta ihe nlele ọbara nwere nje Zika na-adịghị mma, nsonaazụ ya adịghịkwa mma. Nsonaazụ nnwale nnyonye anya na-egosi na mgbe ntinye bilirubin na serum erughị 168.2μmol/ml, ntinye hemoglobin nke hemolysis mepụtara erughị 130g/L, ntinye lipid ọbara erughị 65mmol/ml, mkpokọta ntinye IgG na serum erughị 5mg/mL, enweghị mmetụta ọ bụla na nje dengue, nje Zika ma ọ bụ nchọpụta nje chikungunya. A na-ahọpụta nje Hepatitis A, nje Hepatitis B, nje Hepatitis C, nje Herpes, nje Eastern equine encephalitis, nje Hantavirus, nje Bunya, nje West Nile na ihe nlele serum genomic mmadụ maka ule cross-reactivity, nsonaazụ ya na-egosikwa na enweghị mmeghachi omume cross n'etiti kit a na nje ndị a kpọtụrụ aha n'elu. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR ABI 7500 n'oge dị adịSistemụ PCR ABI 7500 Ngwa Ngwa Ngwa Oge QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P Oge-N'ezie LightCycler®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Nhọrọ nke 1.
Ngwa Ngwuputa RNA Viral nke QIAamp (52904), Mwepụta ma ọ bụ ihe na-eme ka ihe dị ọcha dị na Nucleic Acid (YDP315-R) nke Tiangen Biotech(Beijing) Co.,Ltd.Mwepụta ahụa ga-ewepụta ya dịka ntuziaka mwepụta si dị, olu mwepụta akwadoro bụ 140 μL, olu mwepụta akwadoro bụ 60 μL.
Nhọrọ nke abụọ.
Ngwa DNA/RNA nke nje Macro & Micro-Test Viral (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). A ga-ewepụta mwepụ ahụ dịka ntuziaka si dị. Olu ihe nlele mwepụ ahụ bụ 200 μL, olu mwepụ akwadoro bụ 80 μL.
