Nje Virus Zika
Aha ngwaahịa
Ngwa HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Asambodo
CE
Epidemiology
Nje virus Zika bụ nke genus Flaviviridae, bụ nje RNA nwere eriri nwere otu eriri nwere dayameta nke 40-70nm.O nwere envelopu, nwere 10794 nucleotides, ma tinye 3419 amino acids.Dị ka genotype si kwuo, a na-ekewa ya na ụdị Africa na ụdị Asia.Ọrịa Zika bụ ọrịa na-efe efe na-egbochi onwe ya nke nje Zika na-ebute, nke a na-ebutekarị site na aru Aedes aegypti anwụnta.Ihe ndị a na-eme n'ụlọ ọgwụ bụ nke kachasị ahụ ọkụ, ọkụ ọkụ, arthralgia ma ọ bụ conjunctivitis, ọ naghị adịkarịkwa egbu egbu.Dị ka Òtù Ahụ Ike Ụwa si kwuo, microcephaly nwa ọhụrụ na ọrịa Guillain-Barre (ọrịa Guillain-Barré) nwere ike jikọta ya na nje virus Zika.
Ọwa
| FAM | Zika nje nucleic acid |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤30℃ & echebe site na ìhè |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | ọbara ọhụrụ |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500ng/μl |
| Nkọwapụta | Nsonaazụ ule enwetara site na ngwa a agaghị emetụta haemoglobin (<800g/L), bilirubin (<700μmol/L), na lipids/triglycerides ọbara (<7mmol/L) n'ọbara. |
| Ngwa ndị ọdabara | Sistemụ PCR ABI 7500 ezigbo oge ABI 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler Sistemụ PCR nke BioRad CFX96, BioRad CFX Opus 96 ezigbo oge PCR Sistemu |
Usoro ọrụ
Nhọrọ 1.
QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction ma ọ bụ Reagent dị ọcha (YDP315-R) nke Tiangen Biotech(Beijing) Co., Ltd.The mmịpụtakwesịrị iwepụta ya dị ka ntuziaka mmịpụta si dị, na olu mmịpụta akwadoro bụ 140 μL na oke elution akwadoro bụ 60 μL.
Nhọrọ 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).Ekwesịrị ịwepụta mmịpụta dịka ntuziaka ahụ si dị.Ihe nlele nke mmịpụta bụ 200 μL, na olu elution akwadoro bụ 80μL.
