Nje Virus Zika
Aha ngwaahịa
Ngwa HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Asambodo
CE
Epidemiology
Nje virus Zika bụ nke genus Flaviviridae, bụ nje RNA nwere eriri nwere otu eriri nwere dayameta nke 40-70nm. O nwere envelopu, nwere 10794 nucleotides, ma tinye 3419 amino acids. Dị ka genotype si kwuo, a na-ekewa ya na ụdị Africa na ụdị Asia. Ọrịa Zika bụ ọrịa na-efe efe na-egbochi onwe ya nke nje Zika na-ebute, nke a na-ebutekarị site na aru Aedes aegypti anwụnta. Ihe ndị a na-eme n'ụlọ ọgwụ bụ nke kachasị ahụ ọkụ, ọkụ ọkụ, arthralgia ma ọ bụ conjunctivitis, ọ naghị adịkarịkwa egbu egbu. Dị ka Òtù Ahụ Ike Ụwa si kwuo, microcephaly nwa ọhụrụ na ọrịa Guillain-Barre (ọrịa Guillain-Barré) nwere ike jikọta ya na nje virus Zika.
Ọwa
| FAM | Zika nje nucleic acid |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤30℃ & echebe site na ìhè |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | ọbara ọhụrụ |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 oyiri/ml |
| Nkọwapụta | Jiri ngwa ahụ chọpụta ihe nlele ọbara na nje Zika adịghị mma, nsonaazụ ya adịghị mma. Nsonaazụ nnyonye anya na-egosi na mgbe ntinye nke bilirubin na serum na-erughị 168.2μmol / ml, mkpokọta hemoglobin nke hemolysis na-emepụta abụghị ihe karịrị 130g/L, ntinye nke lipid ọbara abụghị ihe karịrị 65mmol/ml, mkpokọta IgG na serum abụghị ihe karịrị 5mg/mL, ọ dịghị ihe na-akpata nje virus dengue ma ọ bụ Zikun. A na-ahọrọ nje ịba ọcha n'anya, nje ịba ọcha n'anya B, nje ịba ọcha n'anya C, nje Herpes, nje virus nke Eastern equine encephalitis, Hantavirus, Bunya virus, West Nile virus na mkpụrụ ndụ ihe nketa mkpụrụ ndụ mmadụ ka a na-ahọrọ maka ule mmeghachi omume cross-reactivity, na nsonaazụ ya gosiri na ọ dịghị mmeghachi omume dị n'etiti ngwa a na ọrịa nje ndị a kpọtụrụ aha n'elu. |
| Ngwa ndị ọdabara | Sistemụ PCR ABI 7500 ezigbo ogeABI 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®480 ezigbo oge PCR usoro Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1.
QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction ma ọ bụ Reagent dị ọcha (YDP315-R) nke Tiangen Biotech(Beijing) Co., Ltd.The mmịpụtaekwesịrị ịwepụta ya dị ka ntuziaka mmịpụta si dị, na olu mmịpụta akwadoro bụ 140 μL na oke elution akwadoro bụ 60 μL.
Nhọrọ 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ekwesịrị ịwepụta mmịpụta dịka ntuziaka ahụ si dị. Ihe nlele nke mmịpụta bụ 200 μL, na olu elution akwadoro bụ 80μL.
