Ụdị 12 nke ọrịa iku ume
Aha ngwaahịa
HWTS-RT071A 12 ụdị ngwa ihe nchọpụta ihe na-ahụ maka iku ume (Fluorescence PCR)
Ọwa
| Ọwa | hu12Ihe nchekwa mmeghachi omume A | hu12Ihe nchekwa mmeghachi omume B | hu12Ihe nchekwa mmeghachi omume C | hu12Ihe nchekwa mmeghachi omume D |
| FAM | SARS-CoV-2 | HADV | HPIV bụ ọrịa na-efe efe | HRV |
| VIC/HEX | Njikwa ime | Njikwa ime | HPIV bụ ọrịa na-efe efe | Njikwa ime |
| CY5 | IFV A | MP | HPIV bụ ọrịa na-efe efe | / |
| ROX | IFV B | RSV | HPIV bụ ọrịa na-efe efe | HMPV |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | SARS-CoV-2: 300 oyiri/mlnje influenza B: 500 oyiri/mlnje influenza A: 500 Copy/ml Adenovirus: 500 oyiri/ml mycoplasma pneumoniae: 500 oyiri / ml nje syncytial iku ume: 500 oyiri / ml, nje parainfluenza (Ⅰ, Ⅱ, Ⅲ, Ⅳ)：500 oyiri/mL Rhinovirus: 500 oyiri/ml metapneumovirus mmadụ: 500 oyiri/ml |
| Nkọwapụta | Nnyocha nyocha nke cross-reactivity na-egosi na ọ dịghị cross-reactivity n'etiti kit a na enterovirus A, B, C, D, epstein-barr virus, measles virus, mmadụ cytomegalovirus, rotavirus, norovirus, mumps virus, varicella-herpes zoster virus. bordetella pertussis, streptococcus pyogenes, mycobacterium ụkwara nta, aspergillus fumigatus, candida albicans, candida glabrata, pneumocystis jirovecii, cryptococcus neoformans na mmadụ genomic nucleic acid. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR SistemuNgwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugboQuantStudio®Sistemụ PCR 5 ezigbo oge SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 ezigbo oge PCR usoro, Sistemụ nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nhọrọ 1.
akwadoro mmịpụta reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Ekwesịrị ime mmịpụta ahụ n'ụzọ siri ike dabere na ntuziaka.
Nhọrọ 2.
Na-atụ aro mmịpụta reagent: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Akpaaka Nucleic Acid Extractor ( HWTS-3006C, HWTS-3006B) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., a ga-eduzi mmịpụta dịka ntuziaka siri dị.Oke elution akwadoro bụ 80μL.
Nhọrọ 3.
Na-atụ aro mmịpụta reagent: Nucleic Acid mmịpụta ma ọ bụ dị ọcha Kit (YDP315) site Tiangen Biotech(Beijing) Co., Ltd., mmịpụta ga-eduzi dị ka ntuziaka siri ike.Olu elution akwadoro bụ 100μL.
