Ụdị Ọrịa Iku Oku Iri na Abụọ
Aha ngwaahịa
HWTS-RT071A Ụdị Ngwa Nchọpụta Asịd Nucleic Iri na Abụọ nke Ọrịa Iku Oku (Fluorescence PCR)
Ọwa
| Ọwa | hu12Nchekwa Mmeghachi omume A | hu12Nchekwa Mmeghachi omume B | hu12Nchekwa Mmeghachi Omume C | hu12Nchekwa Mmeghachi omume D |
| FAM | SARS-CoV-2 | HADV | Ọrịa HPIV Ⅰ | HRV |
| VIC/HEX | Njikwa Dị n'ime | Njikwa Dị n'ime | HPIV Ⅱ | Njikwa Dị n'ime |
| CY5 | IFV A | MP | Ọrịa HPIV Ⅲ | / |
| ROX | IFV B | RSV | Ọrịa HPIV Ⅳ | HMPV |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | Swab nke oropharyngeal |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | SARS-CoV-2：Mpempe 300/mLỌrịa influenza B: 500 Mpịakọta/mLỌrịa influenza A: 500 Mpempe akwụkwọ/mL Adenovirus: 500 mbipụta/mL Ọrịa oyi nke mycoplasma: 500 Mkpọ/mL nje ọrịa iku ume: 500 Copies/mL, nje parainfluenza (Ⅰ, Ⅱ, Ⅲ, Ⅳ): 500 Mpempe akwụkwọ/mL Rhinovirus: 500 mbipụta/mL ọrịa metapneumovirus mmadụ: 500 mbipụta/mL |
| Nkọwapụta | Nnyocha mmeghachi omume nke cross-reactivity na-egosi na enweghị mmeghachi omume cross-reactivity n'etiti ngwa a na enterovirus A, B, C, D, epstein-barr virus, measles virus, human cytomegalovirus, rotavirus, norovirus, mumps virus, varicella-herpes zoster virus, bordetella pertussis, streptococcus pyogenes, mycobacterium tuberculosis, aspergillus fumigatus, candida albicans, candida glabrata, pneumocystis jiravecii, cryptococcus neoformans na human genomic nucleic acid. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyereSistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa NgwaQuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P Real-Time (Hongshi Medical Technology Co., Ltd.) LightCycler®Sistemụ PCR oge 480, Sistemụ Nchọpụta PCR LineGene 9600 Plus nke Oge N'ezie (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya (Suzhou Molarray Co., Ltd.) Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Nhọrọ nke 1.
Ihe a tụrụ aro maka mwepụta: Ihe a na-akpọ Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., A ga-eme mwepụ ahụ dịka ntuziaka si dị.
Nhọrọ nke abụọ.
Ihe na-eme ka a na-ewepụta ihe dị ka Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., a ga-eme mwepụta ahụ dịka ntuziaka si dị. Olu mwepụta ahụ akwadoro bụ 80μL.
Nhọrọ nke 3.
Ihe a na-atụ aro maka mwepụta: Ngwa mwepụta ma ọ bụ ihe nhicha Nucleic Acid (YDP315) nke Tiangen Biotech(Beijing) Co., Ltd., a ga-eme mwepụta ahụ dịka ntuziaka si dị. Olu mwepụta ahụ akwadoro bụ 100μL.
