Ụdị 17 nke HPV (16/18/6/11/44 Mpempe akwụkwọ)
Aha ngwaahịa
HWTS-CC015 17 Ụdị Papillomavirus mmadụ (16/18/6/11/44) Ngwa nchọpụta Nucleic Acid (Fluorescence PCR)
Epidemiology
Ọrịa kansa cervical bụ otu n'ime etuto ọjọọ na-adịkarị na traktị ọmụmụ ụmụ nwanyị.E gosiputara na ọrịa HPV na-adịgide adịgide na ọtụtụ ọrịa na-efe efe bụ otu n'ime isi ihe na-ebute ọrịa kansa cervical.Ka ọ dị ugbu a, a ka enwekwaghị ọgwụgwọ dị mma nke a na-anabatakarị maka ọrịa kansa cervical nke HPV kpatara.Ya mere, nchọpụta mmalite na mgbochi ọrịa cervical nke HPV kpatara bụ igodo iji gbochie ọrịa cancer nke cervical.Ịmepụta ule nyocha dị mfe, nke akọwapụtara na ngwa ngwa maka ọrịa nje dị oke mkpa maka nyocha ụlọ ọgwụ nke ọrịa cancer cervical.
Ọwa
| PCR-Mix1 | FAM | 18 |
| VIC/HEX | 16 | |
| ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Njikwa ime |
| PCR-Mix2 | FAM | 6 |
| VIC/HEX | 11 | |
| ROX | 44 | |
| CY5 | Njikwa ime |
Nka nka
| Nchekwa | -18 ℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Ihe nlele mmamịrị, ihe gbasara swab nke nwanyị, ihe swab nke nwanyị |
| Ct | ≤28 |
| LoD | 300 oyiri/ml |
| Nkọwapụta | Ọ dịghị cross-reactivity na Ureaplasma urealyticum, Chlamydia trachomatis nke ọmụmụ tract, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, ebu , Gardnerella na ndị ọzọ HPV ụdị adịghị ekpuchi site kit. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge Sistemụ PCR SLAN-96P ezigbo oge LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke nwere ike iji na Macro & Micro-Test). Akpaaka Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tinye 200μL nke saline nkịtị iji kwughachi pellet ahụ na nzọụkwụ 2.1, mgbe ahụ, a ga-eme mmịpụta ahụ dịka ọ dị. na ntuziaka maka ojiji nke a mmịpụta reagent.Oke elution akwadoro bụ 80μL.
Reagenti mmịpụta akwadoro: QIAamp DNA Mini Kit (51304) ma ọ bụ kọlụm Macro & Micro-Test Viral DNA/RNA (HWTS-3020-50).Tinye 200μL nke saline nkịtị ka ịmaliteghachi pellet ahụ na nzọụkwụ 2.1, mgbe ahụ, a ga-eme mmịpụta ahụ dịka ntuziaka maka ojiji nke reagent mmịpụta a.The amịpụtara sample olu nke sample bụ niile 200μL, na akwadoro elution olu bụ 100μL.
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Sample Mwepụta Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L).Tinye 200μL nke reagent ntọhapụ ka ịmaliteghachi pellet na nzọụkwụ 2.1, wee mee mmịpụta ahụ dịka ntuziaka maka ojiji nke reagent mmịpụta a.
