Ụdị HPV iri na asaa (16/18/6/11/44 Ide ihe)
Aha ngwaahịa
HWTS-CC015 Ụdị 17 nke Papillomavirus Mmadụ (16/18/6/11/44 Ịpịpụta) Ngwa Nchọpụta Acid Nucleic (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa kansa olu bụ otu n'ime etuto ọjọọ kachasị na-eme n'ime ụmụ nwanyị. Egosila na ọrịa HPV na-adịgide adịgide na ọtụtụ ọrịa bụ otu n'ime ihe ndị bụ isi na-akpata ọrịa kansa olu. Ugbu a, enwere enweghị ọgwụgwọ dị irè a na-anabatakarị maka ọrịa kansa olu nke HPV na-akpata. Ya mere, nchọpụta mbụ na mgbochi nke ọrịa olu nke HPV na-akpata bụ isi ihe na-egbochi ọrịa kansa olu. Ịmepụta ule nchọpụta dị mfe, kpọmkwem na ngwa ngwa maka nje dị oke mkpa maka nchọpụta ọrịa kansa olu.
Ọwa
| PCR-Mix1 | FAM | 18 |
| VIC/HEX | 16 | |
| ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Njikwa dị n'ime |
| PCR-Mix2 | FAM | 6 |
| VIC/HEX | 11 | |
| ROX | 44 | |
| CY5 | Njikwa dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | -18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | Ihe nlele mmamịrị, ihe nlele swab nke akpanwa nwanyị, ihe nlele swab nke akpanwa nwanyị |
| Ct | ≤28 |
| LoD | 300Mpempe akwụkwọ/mL |
| Nkọwapụta | Enweghị mmeghachi omume na Ureaplasma urealyticum, Chlamydia trachomatis nke usoro ọmụmụ, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mold, Gardnerella na ụdị HPV ndị ọzọ nke ngwa ahụ na-ekpuchighị. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P Oge-N'ezie LightCycler®Sistemụ PCR 480 n'oge a Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Ihe na-eme ka a na-ewepụta ihe: Ngwa DNA/RNA nke Macro & Micro-Test Viral (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke enwere ike iji Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tinye 200μL nke saline nkịtị iji weghachite pellet ahụ na nzọụkwụ 2.1, wee mee mwepụ ahụ dịka ntuziaka maka ojiji nke ihe na-eme ka a na-ewepụta ihe a si dị. Olu mwepụta akwadoro bụ 80μL.
Ihe na-eme ka a na-ewepụta ihe a tụrụ aro: QIAamp DNA Mini Kit (51304) ma ọ bụ Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Tinye 200μL nke saline nkịtị iji weghachite pellet ahụ na nzọụkwụ 2.1, wee mee mwepụ ahụ dịka ntuziaka maka ojiji nke ihe na-eme ka a na-ewepụta ihe a si dị. Olu ihe nlele ahụ niile bụ 200μL, olu ihe nlele ahụ akwadoro bụ 100μL.
Ihe a na-atụ aro maka mwepụta: Ihe a na-akpọ Macro & Micro-Test Sample Release Release Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L). Tinye 200μL nke ihe a na-akpọ sample reagent iji weghachite pellet ahụ na nzọụkwụ 2.1, wee mee mwepụta ahụ dịka ntuziaka maka ojiji nke ihe a na-akpọ extraction a si dị.
