Ụdị Nje Virus Anọ nke Iku Oku
Aha ngwaahịa
HWTS-RT099- Ụdị Nje Virus Iku Oku Anọ Ngwa Nchọpụta Asịd Nucleic (Fluorescence PCR)- Sistemụ PCR NED-ABI 7500 Real-Time/Sistemụ PCR ABI 7500 Ngwa Ngwa Ngwa/QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ
Ụdị Nje Virus Iku Ume HWTS-RT158-4 Ngwa Nchọpụta Asịd Nucleic (Fluorescence PCR)Quasar 705
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa Coronavirus nke afọ 2019, nke a na-akpọ "COVID-19", na-ezo aka na oyi baa nke sitere na ya.2019-nCoVọrịa.2019-nCoVbụ coronavirus nke sitere na β genus. COVID-19 bụ ọrịa na-efe efe nke na-efe efe n'ikuku, ndị mmadụ na-enwekarịkwa ike ibute ya. Ugbu a, isi ihe kpatara ọrịa ahụ bụ ndị ọrịa butere ya site na nje ahụ.2019-nCoV, ndị nwere ọrịa na-enweghị ihe mgbaàmà nwekwara ike ịghọ isi iyi nke ọrịa ahụ. Dabere na nyocha ọrịa na-efe efe ugbu a, oge mmalite nke iku ume bụ ụbọchị 1-14, ọkachasị ụbọchị 3-7. Ahụ ọkụ, ụkwara akọrọ na ike ọgwụgwụ bụ ihe mgbaàmà bụ́ isi. Ụfọdụ ndị ọrịa nwere ihe mgbaàmà ahụ.dị kaimi mkpọchi, imi mmiri na-agba, akpịrị na-afụ ụfụ, myalgia na afọ ọsịsa, wdg.
Ọwa
| FAM | 2019-nCoV |
| VIC (HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | -18℃ |
| Ndụ nchekwa | Ọnwa itoolu |
| Ụdị ihe atụ | Swab nke oropharyngeal |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Mpempe/mLỌrịa Influenza A/Nje Influenza B/Nje syncytial nke iku ume: 500Isi/mL |
| Nkọwapụta | a) Nsonaazụ mmeghachi omume na-egosi na enweghị mmeghachi omume mmegharị n'etiti kit na nje coronavirus mmadụ SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, ụdị nje parainfluenza 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, nje metapneumovirus mmadụ, enterovirus A, B, C, D, nje pulmonary mmadụ, nje epstein-barr, nje measles, nje cytomegalo mmadụ, rotavirus, norovirus, nje parotitis, nje varicella-zoster, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci na ọhụrụ cryptococcus na human genomic nucleic acid. b) Ike igbochi ihe mgbochi: select mucin (60mg/mL), 10% (v/v) nke ọbara na phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (gụnyere ihe nchekwa) (20 mg/mL), beclomethasone (20 mg/mL), dexamethasone (20 mg/mL), flunisolide (20 mg/mL), triamcinolone acetonide (2 mg/mL), budesonide (2 mg/mL), mometasone (2 mg/mL), fluticasone (2 mg/mL), histamine hydrochloride (5 mg/mL), alpha interferon (800 IU/mL), zanamivir (20 mg/mL), ribavirin (10 mg/mL), oseltamivir (60 mg/mL), peramivir (1 mg/mL), lopinavir (500 mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) na tobramycin (0.6mg/mL) maka nnwale nnyonye anya, nsonaazụ ya na-egosikwa na ihe ndị na-egbochi ndị nwere oke ahụ e kwuru n'elu enweghị mmeghachi omume nnyonye anya na nsonaazụ nnwale nke nje. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyereSistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa Sistemụ PCR nke QuantStudio®5 n'oge dị adị |
Usoro Ọrụ
Nhọrọ nke 1.
Ngwa DNA/RNA nke nje Macro & Micro-Test Viral (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd mepụtara. Olu ihe nlele ewepụtara bụ 200μL, olu ihe mgbapụta akwadoro bụ 80μL.
Nhọrọ nke abụọ.
Ngwa QIAamp Viral RNA Mini Kit (52904) nke QIAGEN ma ọ bụ Nucleic Acid Extraction or Purification Kit (YDP315-R) nke Tiangen Biotech (Beijing) Co., Ltd mepụtara. Olu ihe nlele ewepụtara bụ 140μL, olu mgbapụta akwadoro bụ 60μL.
