Ụdị nje 4 na-eku ume
Aha ngwaahịa
HWTS-RT099-4 ụdị nke nje virus iku ume Nucleic Acid Detection Kit (Fluorescence PCR) - NED-ABI 7500 PCR Real Time System/ABI 7500 Ngwa ngwa PCR Systems/QuantStudio®Sistemụ PCR 5 ezigbo oge
HWTS-RT158-4 Ụdị nke Nje Virus iku ume Nucleic Acid Detection Kit (Fluorescence PCR) -Quasar 705
Epidemiology
Ọrịa Corona Virus 2019, nke akpọrọ "COVID-19", na-ezo aka na oyi baa na-akpata.2019-nCoVọrịa.2019-nCoVbụ coronavirus bụ nke β genus. COVID-19 bụ ọrịa na-efe efe na-efe efe na-efe efe na-efe efe, ndị mmadụ na-enwekarịkwa ike. Ka ọ dị ugbu a, isi mmalite nke ọrịa bụ ndị ọrịa butere ya2019-nCoV, na ndị butere ọrịa asymptomatic nwekwara ike bụrụ isi mmalite nke ọrịa. Dabere na nyocha ọrịa ọrịa ugbu a, oge nnabata bụ ụbọchị 1-14, ọkachasị ụbọchị 3-7. Ahụ ọkụ, ụkwara akọrọ na ike ọgwụgwụ bụ isi ihe ngosi. Ndị ọrịa ole na ole nwere akaras dị kaimi imi, imi imi, akpịrị akpịrị, myalgia na afọ ọsịsa, wdg.
Ọwa
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Njikwa ime |
Nka nka
| Nchekwa | -18 ℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300 oyiri/mlInfluenza A virus/Influenza B nje/Nje virus syncytial iku ume: 500Copy/mL |
| Nkọwapụta | a) Cross-reactivity results na-egosi na ọ dịghị cross mmeghachi omume n'etiti kit na mmadụ coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza nje ụdị 1, 2, 3, rhinovirus A, B, diamyvirus chulamoni, C enterovirus A, B, C, D, nje virus akpa ume mmadụ, nje epstein-barr, nje measles, nje cytomegalo mmadụ, rotavirus, norovirus, nje parotitis, nje virus varicella-zoster, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aures aureus, strepylococcus aures, pstreuptococcus aureus klebsiella pneumoniae, mycobacterium ụkwara nta, anwụrụ ọkụ aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci na amụrụ ọhụrụ cryptococcus na mmadụ genomic nucleic acid. b) Ikike mgbochi nnyonye anya: họrọ mucin (60mg / ml), 10% (v / v) nke ọbara na phenylephrine (2mg / mL), oxymetazoline (2mg / ml), sodium chloride (gụnyere preservatives) (20 mg / ml), beclomethasone (20mg / mL), 20 mg / ml), dexamnisone. (20μg / ml), triamcinolone acetonide (2mg / ml), budesonide (2mg / ml), mometasone (2mg / ml), fluticasone (2mg / ml), histamine hydrochloride (5mg / ml), alpha interferon (800IU / ml), zanamivir (20mg/mL) , ovarim ribavirin. (60ng / ml), peramivir (1mg / ml), lopinavir (500mg / ml), ritonavir (60mg / mL), mupirocin (20mg / ml), azithromycin (1mg / ml), ceftriaxone (40μg / ml), meropenem (200mg / ml / ml), 10μcin na levocin. (0.6mg / mL) maka nyocha nnyonye anya, nsonaazụ ya na-egosi na ihe ndị na-etinye aka na ntinye aka ndị a kpọtụrụ aha n'elu enweghị mmeghachi omume nnyonye anya na nsonaazụ ule nke nje. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCRNgwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR Real-Time QuantStudio®5 |
Usoro ọrụ
Nhọrọ 1.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) nke Jiangsu Macro & Micro-Test Med-Tech Co., akwadoro, bụ eluL. olu bụ 80 μl.
Nhọrọ 2.
QIAamp Viral RNA Mini Kit (52904) mepụtara site na QIAGEN ma ọ bụ Nucleic Acid Extraction or Purification Kit (YDP315-R) nke Tiangen Biotech (Beijing) Co., Ltd mepụtara. Ihe nlele amịpụtara bụ 140μL, na olu elution akwadoro bụ 60μL.
