Ngwa Nchọpụta Acid Nucleic nke Nje Virus Dengue I/II/II/IV nke a kpọrọ nkụ (Fluorescence PCR)-RUO
Aha ngwaahịa
Ngwa Nchọpụta Acid Nucleic nke Nje Virus Dengue I/II/II/IV nke a kpọrọ nkụ (Fluorescence PCR)-RUO
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa Dengue (DF), nke ọrịa denguevirus (DENV) na-akpata, bụ otu n'ime ọrịa ndị na-efe efe kachasị na ọrịa arbovirus. Usoro mbufe ya gụnyere Aedes aegypti na Aedes albopictus. DF na-adịkarị na mpaghara okpomọkụ na nke dị n'okpuru okpomọkụ. DENV bụ nke flavivirus n'okpuru flaviviridae, enwere ike kewaa ya n'ime ụdị anọ dịka antigen dị n'elu si dị. Ngosipụta ahụike nke ọrịa DENV gụnyere isi ọwụwa, ahụ ọkụ, adịghị ike, mmụba nke lymph node, leukopenia na wdg, na ọbara ọgbụgba, ujo, mmerụ ahụ imeju ma ọ bụ ọbụna ọnwụ n'ọnọdụ ndị siri ike. N'afọ ndị na-adịbeghị anya, mgbanwe ihu igwe, mmepe obodo, mmepe ngwa ngwa nke njem nlegharị anya na ihe ndị ọzọ enyela ọnọdụ ngwa ngwa na dị mfe maka izipu na mgbasa nke DF, na-eduga na mgbasawanye nke mpaghara ọrịa DF mgbe niile. Ya mere, ịmepụta ụzọ nchọpụta etiological dị mfe, kpọmkwem na ngwa ngwa nke DENV dị mkpa maka nchọpụta ahụike nke DF.
Usoro Nkà na ụzụ
| Nchekwa | 2-8℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | serum |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Mbipụta 500/mL |
| Nkọwapụta | Nnwale ahụ adịghị mma maka ihe nlele serum nke nje dengue na-adịghị mma; nsonaazụ nnwale nnyonye anya na-egosi na mgbe ntinye bilirubin na serum adịghị karịa 168.2μmol/mL, ntinye nke hemoglobin nke hemolysis mepụtara abụghị ihe karịrị 130g/L, ntinye lipid ọbara adịghị karịa 65mmol/mL, na mkpokọta ntinye IgG na serum erughị 5mg/mL, enweghị mmetụta ọ bụla na nchọpụta nke nje dengue; jiri nje Hepatitis A, nje Hepatitis B, nje Hepatitis C, nje Herpes, nje Eastern equine encephalitis, nje Hantaan, nje Bunia, nje West Nile, nje Zika na ihe nlele serum genomic mmadụ maka ule cross-reactivity, nsonaazụ ya na-egosi na enweghị mmeghachi omume cross n'etiti ngwa a na nje ndị dị n'elu. |
| Ngwa Ndị Dị Mkpa | Ụdị nke Mbụ: Sistemụ Biosystems 7500 Real-Time PCR, Sistemụ QuantStudio®5 Real-Time PCR, Sistemụ SLAN-96P Real-Time PCR (Hongshi Medical Technology Co., Ltd.), Sistemụ Nchọpụta LineGene 9600 Plus Real-Time PCR (FQD-96A, teknụzụ Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Sistemụ BioRad CFX96 Real-Time PCR, Sistemụ BioRad CFX Opus 96 Real-Time PCR. Ụdị nke Abụọ: EudemonTMAIO800 (HWTS-EQ007) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
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Usoro Ọrụ
Ihe ndị na-eme ka ihe na-eme ka ihe na-eme ka ihe dị n'ime mmiri chọrọ mana enyeghị ha:
Ụdị nke Mbụ: Ngwa Ngwa DNA/RNA nke Macro & Micro-Nnwale General (HWTS-3017) (nke enwere ike iji ya na Macro & Micro-Nnwale Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Nnwale Viral DNA/RNA Column (HWTS-3022).
Ụdị nke Abụọ: Ngwa DNA/RNA nke Macro & Micro-Nnwale General (HWTS-3023-8), enwere ike iji ya na EudemonTM AIO800 (HWTS-EQ007) site na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tupu ejiri ihe nnwale Ụdị nke Abụọ, onye ahịa nwere ihe na-akwado mwepụta na ngwa mmụba, onye injinia ga-eduzi onye ahịa ka ọ rụọ ọrụ nnwale ahụ mgbe ọ na-eji ya oge mbụ.
A chọrọ ihe oriri mana enyeghị ha:Ntuziaka DNase/RNase Enweghị, uwe aka a na-atụfu atụfu, DNase/RNase Tubụ EP efu, centrifuge, ihe ndọta, mpempe tube 8 maka PCR.
