Ihe nje iku ume isii akpọnwụrụ akpọnwụrụ nucleic acid
Aha ngwaahịa
Ngwa HWTS-RT192-Freeze-akpọnwụrụ ngwa ngwa nchọta iku ume isii (Fluorescence PCR)
Epidemiology
Ọrịa iku ume bụ ụdị ọrịa mmadụ a na-ahụkarị, nke nwere ike ime na okike, afọ na mpaghara ọ bụla, ma bụrụ otu n'ime ihe kacha mkpa na-ebute ọrịa na ọnwụ n'ụwa [1]. Ọrịa iku ume na-ahụkarị n'ụlọ ọgwụ gụnyere nje syncytial iku ume, adenovirus, metapneumovirus mmadụ, rhinovirus, nje parainfluenza (I/II/III) na mycoplasma pneumoniae, wdg [2,3]. Mgbaàmà nke ụlọ ọgwụ na ihe ịrịba ama nke ọrịa iku ume na-akpata yiri nke ahụ, ma ọrịa nke ọrịa dị iche iche na-akpata nwere usoro ọgwụgwọ dị iche iche, mmetụta ọgwụgwọ na ọrịa [4,5]. Ka ọ dị ugbu a, ụzọ ndị bụ isi nke nchọpụta ụlọ nyocha nke ọrịa iku ume na-agụnye: iche iche nje, nchọpụta antigen na nchọpụta nucleic acid, wdg. Ngwa a na-achọpụta ma na-achọpụta kpọmkwem viral nucleic acids na ndị mmadụ nwere ihe ịrịba ama na mgbaàmà nke ọrịa iku ume, na njikọ ndị ọzọ na-adakarị na ụlọ nyocha na-enyere aka na nchọpụta nke ọrịa iku ume.
Nka nka
| Nchekwa | 2-28℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Nasopharyngeal swab |
| Ct | RSV,Adv,hMPV,Rhv,PIV,MP Ct≤35 |
| LoD | 200 mbipụta/ml |
| Nkọwapụta | Cross reactivity: Ọ dịghị cross reactivity n'etiti kit na Boca nje, Cytomegalovirus, Epstein-Barr virus, Herpes simplex virus, varicella zoster Virus, Mumps virus, Enterovirus, measles virus, mmadụ coronavirus, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, pneptococcusiella, Streptococcusiella. ka oyi baa, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium ụkwara nta, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptoansssalicoccus nephrine catarrh, Lactobacillus, Corynebacterium, DNA genomic mmadụ. |
| Ngwa ndị ọdabara | Ọdabara na ụdị I test reagent: Ngwa Biosystem 7500 ezigbo oge PCR, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.). Ọdabara na ụdị II test reagent: EudemonTMAIO800 (HWTS-EQ007) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Usoro ọrụ
PCR nkịtị
Macro & Micro-Test General DNA / RNA Kit (HWTS-3019) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B)) site na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. na-akwadoro maka nlele anya n'okpuru usoro nke FU.
AIO800 niile-na-otu igwe
