Mgbanwe Mkpụrụ Ndụ Ihe nketa CYP2C19 nke Mmadụ
Aha ngwaahịa
Ngwa Nchọpụta Mkpụrụ Ndụ Mkpụrụ Ndụ HWTS-GE012A-mmadụ CYP2C19 (Fluorescence PCR)
Asambodo
CE/TFDA
Ọmụmụ ihe gbasara ọrịa na-efe efe
CYP2C19 bụ otu n'ime enzymes dị mkpa na-eme ka ọgwụ dị iche iche na ezinụlọ CYP450. Ọtụtụ ihe ndị dị n'ime endogenous na ihe dị ka 2% nke ọgwụ ndị a na-eme ka CYP2C19 dị iche, dịka metabolism nke ndị na-egbochi njikọta ihe mgbochi (dịka clopidogrel), ndị na-egbochi proton pump (omeprazole), ndị na-egbochi nkụchi obi, wdg. Mkpụrụ ndụ ihe nketa CYP2C19 nwekwara ọdịiche dị na ikike nhazi nke ọgwụ ndị metụtara ya. Mgbanwe isi ndị a nke *2 (rs4244285) na *3 (rs4986893) na-akpata mfu nke ọrụ enzyme nke mkpụrụ ndụ ihe nketa CYP2C19 dere na adịghị ike nke ikike nhazi metabolic, ma na-eme ka ntinye ọbara dịkwuo elu, iji mee ka mmeghachi omume ọgwụ ọjọọ metụtara ntinye ọbara dịkwuo njọ. *17 (rs12248560) nwere ike ịbawanye ọrụ enzyme nke mkpụrụ ndụ ihe nketa CYP2C19 dere, mmepụta nke metabolites na-arụ ọrụ, ma melite mgbochi nchịkọta platelet ma mee ka ihe egwu ọbara dị elu. Maka ndị na-eji ọgwụ eme ihe nwayọ nwayọ, ịṅụ ọgwụ nkịtị ruo ogologo oge ga-akpata nnukwu nsí na mmetụta ọjọọ: ọkachasị mmebi imeju, mmebi sistemụ hematopoietic, mmebi sistemụ akwara etiti, wdg, nke nwere ike ibute ọnwụ n'ọnọdụ ndị siri ike. Dịka ọdịiche dị n'etiti onwe onye na metabolism ọgwụ kwekọrọ, a na-ekewa ya n'ọtụtụ ụzọ anọ, ya bụ metabolism ngwa ngwa (UM,*17/*17,*1/*17), metabolism ngwa ngwa (RM,*1/*1), metabolism etiti (IM, *1/*2, *1/*3), metabolism nwayọ nwayọ (PM, *2/*2, *2/*3, *3/*3).
Ọwa
| FAM | CYP2C19*2 |
| CY5 | CYP2C9*3 |
| ROX | CYP2C19*17 |
| VIC/HEX | IC |
Usoro Nkà na ụzụ
| Nchekwa | Mmiri mmiri: ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | Ọbara ọhụrụ nke EDTA anticoagulated |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Nkọwapụta | Enweghị mmeghachi omume na-agbanwe agbanwe na usoro ndị ọzọ na-agbanwe agbanwe (mkpụrụ ndụ ihe nketa CYP2C9) na mkpụrụ ndụ ihe nketa mmadụ. Mgbanwe nke ebe CYP2C19*23, CYP2C19*24 na CYP2C19*25 dị n'èzí oke nchọpụta nke ngwa a enweghị mmetụta ọ bụla na mmetụta nchọpụta nke ngwa a. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa Sistemụ PCR nke QuantStudio®5 n'oge dị adị Sistemụ PCR SLAN-96P Oge-N'ezie Sistemụ PCR oge-oge LightCycler®480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Ihe na-eme ka a na-ewepụta ihe a tụrụ aro: Ngwakọta DNA/RNA nke Macro & Micro-Test General (HWTS-3019) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekwesịrị iwepụta ihe a dịka ntuziaka si dị. Olu ihe nlele ahụ bụ 200μL, olu mgbapụta ahụ akwadoro bụ 100μL.
A ga-ewepụta ihe na-eme ka a na-ewepụta ihe na-eme ka a na-ewepụta ihe na-eme ka a na-ewepụta ihe na-eme ka a na-ewepụta ihe na-eme ka a na-ewepụta ihe na-eme ka a na-ewepụta ihe na-eme ka a na-ewepụta ihe na-eme ka a na-ewepụta ihe na-eme ka ihe dị na ya pụta ìhè: Ngwa Wizard® Genomic DNA Purification Kit (Nọmba Katalọgụ: A1120) nke Promega, Nucleic Acid Extraction or Purification Reagent (YDP348) nke Tiangen Biotech(Beijing) Co.,Ltd. dịka ntuziaka mwepụta si dị, olu mwepụta akwadoro bụ 200 μL yana olu mwepụta akwadoro bụ 160 μL.
