Mmadụ TEL-AML1 Fusion Gene Mutation
Aha ngwaahịa
HWTS-TM016 mmadụ TEL-AML1 Ngwa nchọpụta mgbanwe mkpụrụ ndụ (Fluorescence PCR)
Epidemiology
Nnukwu ọrịa leukemia lymphoblastic (ALL) bụ ajọ ọrịa na-adịkarị n'oge nwata.N'ime afọ ndị na-adịbeghị anya, nnukwu ọrịa leukemia (AL) agbanweela site na ụdị MIC (morphology, immunology, cytogenetics) gaa na ụdị MICM (mgbakwunye nke nyocha ihe ọmụmụ ihe omimi).N'afọ 1994, a chọpụtara na njikọ TEL n'oge ọ bụ nwata sitere na ntụgharị chromosomal na-abụghị nke a na-atụghị anya ya bụ t(12;21)(p13;q22) na B-lineage acute lymphoblastic leukemia (ALL).Ebe ọ bụ na achọpụtara mkpụrụ ndụ ihe nketa AML1, TEL-AML1 fusion gene bụ ụzọ kacha mma isi kpee amụma gbasara ụmụaka nwere nnukwu ọrịa leukemia lymphoblastic.
Ọwa
| FAM | Mkpụrụ ndụ ihe nketa TEL-AML1 |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | ụmị ọkpụkpụ sample |
| Ct | ≤40 |
| CV | <5.0% |
| LoD | 1000 oyiri/ml |
| Nkọwapụta | Enweghị mmeghachi omume n'etiti ngwa na mkpụrụ ndụ ihe nketa ndị ọzọ dị ka BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, PML-RARa fusion genes. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR ezigbo oge QuantStudio5 Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Ngwakọta mmịpụta RNA dị ọcha RNAprep (DP433).
