Influenza A Nje Virus Universal/H1/H3
Aha ngwaahịa
HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit (Fluorescence PCR)
Epidemiology
Nje virus influenza bụ ihe nnọchianya nke Orthomyxoviridae. Ọ bụ ọrịa nje na-eyi ahụ́ ike mmadụ egwu nke ukwuu. Ọ nwere ike ibunye onye ọbịa ahụ nke ukwuu. Ọrịa na-efe efe nke oge a na-emetụta ihe dị ka nde mmadụ 600 n'ụwa nile ma na-akpata ọnwụ 250,000 ~ 500,000, nke nje influenza A bụ isi ihe na-ebute ọrịa na ọnwụ. Influenza A bụ nje RNA na-adịghị mma nwere otu eriri. Dị ka elu hemagglutinin (HA) na neuraminidase (NA), HA nwere ike kewaa n'ime 16 subtypes, NA Kewara n'ime 9 subtypes. N'ime nje virus influenza A, ụdị subtypes nke nje influenza nwere ike ibunye mmadụ ozugbo bụ: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 na H10N8. N'ime ha, H1 na H3 subtypes bụ nnukwu ọrịa na-efe efe, ma bụrụ ndị kwesịrị nlebara anya karịsịa.
Ọwa
| FAM | influenza A eluigwe na ala ụdị nje nucleic acid |
| VIC/HEX | influenza A H1 ụdị nje nucleic acid |
| ROX | influenza A H3 ụdị nje nucleic acid |
| CY5 | njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | nasopharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 oyiri/μL |
| Nkọwapụta | Ọ dịghị cross-reactivity na ndị ọzọ akụkụ okuku ume samples dị ka Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneu/B1. nje syncytial A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, wdg na mmadụ genomic DNA. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR Real-Time QuantStudio®5 SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nucleic Acid mmịpụta ma ọ bụ reagent dị ọcha (YDP315-R) nke Tiangen Biotech (Beijing) Co., Ltd. Ekwesịrị ịme mmịpụta ahụ nke ọma dịka ntuziaka maka ojiji. Ihe nlele nke ewepụtara bụ 140μL, na olu elution akwadoro bụ 60μL.
Nhọrọ 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ekwesịrị ịme mmịpụta ahụ nke ọma dịka ntuziaka maka ojiji. Ihe nlele nke ewepụtara bụ 200μL, na olu elution akwadoro bụ 80μL.
