Nje Virus Influenza A zuru ụwa ọnụ/H1/H3
Aha ngwaahịa
Ngwa Nchọpụta Mgbakwunye A nke HWTS-RT012 maka Influenza A (PCR Fluorescence)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Nje Influenza bụ ụdị Orthomyxoviridae nke na-anọchite anya ya. Ọ bụ nje na-efe efe nke na-eyi ahụike mmadụ egwu nke ukwuu. Ọ nwere ike ibute ọrịa ahụ nke ukwuu. Ọrịa oge a na-efe efe na-emetụta ihe dị ka nde mmadụ 600 n'ụwa niile ma na-akpata ọnwụ 250,000 ruo 500,000, nke nje influenza A bụ isi ihe na-akpata ọrịa na ọnwụ. Nje Influenza A bụ RNA nke nwere otu eriri na-adịghị mma. Dịka elu ya si dị, enwere ike kewaa HA n'ụdị 16, NA. Kewara ya n'ụdị 9. N'ime nje influenza A, ụdị nje influenza ndị nwere ike ibute ọrịa na ụmụ mmadụ ozugbo bụ: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 na H10N8. N'ime ha, ụdị H1 na H3 na-ebute ọrịa nke ukwuu, ha kwesịkwara nlebara anya karịsịa.
Ọwa
| FAM | ụdị nje influenza A zuru ụwa ọnụ nke ụdị nucleic acid |
| VIC/HEX | influenza A ụdị H1 nje nucleic acid |
| ROX | influenza A ụdị H3 nje nucleic acid |
| CY5 | njikwa dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa itoolu |
| Ụdị ihe atụ | swab nke nasopharyngeal |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Mbipụta 500/μL |
| Nkọwapụta | Enweghị mmeghachi omume mmegharị ahụ na ihe nlele iku ume ndị ọzọ dịka Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneumovirus A1/A2/B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, wdg. na DNA genomic mmadụ. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa Sistemụ PCR nke QuantStudio®5 n'oge dị adị Sistemụ PCR SLAN-96P nke Oge-N'ezie (Hongshi Medical Technology Co., Ltd.) Sistemụ PCR oge-oge LightCycler®480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya (Suzhou Molarray Co., Ltd.) Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
Mwepụta ma ọ bụ Mwepụta Nsụpụta Asịd Nucleic (YDP315-R) nke Tiangen Biotech (Beijing) Co.,Ltd. A ga-eme mwepụta ahụ kpọmkwem dịka ntuziaka maka ojiji si dị. Olu ihe nlele ewepụtara bụ 140μL, olu mmịpụta akwadoro bụ 60μL.
Nhọrọ nke abụọ.
Ngwa DNA/RNA nke nje Macro & Micro-Test Viral (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). A ga-eme mwepụta ahụ nke ọma dịka ntuziaka maka ojiji si dị. Olu ihe nlele ewepụtara bụ 200μL, olu mgbapụta akwadoro bụ 80μL.
