Influenza A Nje Virus Universal/H1/H3
Aha ngwaahịa
HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit (Fluorescence PCR)
Epidemiology
Mycoplasma pneumoniae (MP) bụ obere prokaryotic microorganism nwere usoro cell na enweghị mgbidi cell n'etiti nje bacteria na nje.MP na-ebutekarị ọrịa iku ume n'ime mmadụ, ọkachasị ụmụaka na ndị ntorobịa.MP nwere ike ibute Mycoplasma hominis oyi baa, ọrịa akụkụ okuku ume na ụmụaka na oyi baa nkịtị.Mgbaàmà nke ụlọ ọgwụ dị iche iche, nke na-abụkarị ụkwara siri ike, ahụ ọkụ, oyi oyi, isi ọwụwa, akpịrị akpịrị, ọrịa akụkụ okuku ume elu na bronchopneumonia bụ ihe a na-ahụkarị.Ụfọdụ ndị ọrịa nwere ike ịmalite nnukwu oyi baa site na ọrịa akụkụ iku ume elu, na oke nsogbu iku ume ma ọ bụ ọbụna ọnwụ nwere ike ime.MP bụ otu n'ime nje ndị na-ahụkarị na ndị dị mkpa na oyi baa na-enweta obodo (CAP), na-aza 10%-30% nke CAP, na oke nwere ike ịbawanye ugboro 3-5 mgbe MP juru ebe niile.N'afọ ndị na-adịbeghị anya, ọnụ ọgụgụ nke MP na CAP pathogens ejirila nwayọọ nwayọọ mụbaa.Mmụba nke ọrịa Mycoplasma pneumoniae abawanyela, na n'ihi ngosipụta ụlọ ọgwụ na-akọwaghị ya, ọ dị mfe nghọta na oyi na-atụ nje na nje.Ya mere, nchọpụta ụlọ nyocha mmalite dị oke mkpa maka nchọpụta ụlọ ọgwụ na ọgwụgwọ.
Ọwa
| FAM | influenza A eluigwe na ala ụdị nje nucleic acid |
| VIC/HEX | influenza A H1 ụdị nje nucleic acid |
| ROX | influenza A H3 ụdị nje nucleic acid |
| CY5 | njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | nasopharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 oyiri/μL |
| Nkọwapụta | Ọ dịghị cross-reactivity na ndị ọzọ akụkụ okuku ume samples dị ka Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echomo2 virus, Metapneu. B1/B2, nje syncytial respiratory A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, wdg na mmadụ genomic DNA. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR ezigbo oge QuantStudio5 SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
Nucleic Acid mmịpụta ma ọ bụ reagent dị ọcha (YDP315-R) nke Tiangen Biotech (Beijing) Co., Ltd.Ekwesịrị ịme mmịpụta ahụ nke ọma dịka ntuziaka maka ojiji.Ihe nlele nke ewepụtara bụ 140μL, na olu elution akwadoro bụ 60μL.
Nhọrọ 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ekwesịrị ịme mmịpụta ahụ nke ọma dịka ntuziaka maka ojiji.Ihe nlele nke ewepụtara bụ 200μL, na olu elution akwadoro bụ 80μL.
