Mycobacterium Tuberculosis Nucleic Acid na Rifampicin (RIF), Nguzogide (INH)

Mycobacterium Tuberculosis Nucleic Acid na Rifampicin (RIF)，Resistance (INH) Foto a egosipụtara

Nkọwa Dị Mkpirikpi:

A na-eji ngwa a maka nchọpụta in vitro nke DNA ụkwara nta Mycobacterium na sputum mmadụ, omenala siri ike (LJ Medium) na omenala mmiri mmiri (MGIT Medium), mmiri ịsa ahụ bronchial, na mgbanwe na mpaghara codon amino acid 507-533 (81bp, mpaghara mkpebi mgbochi rifampicin) nke mkpụrụ ndụ ihe nketa rpoB nke iguzogide ụkwara nta Mycobacterium rifampicin, yana mgbanwe na ebe mgbanwe bụ isi nke iguzogide isoniazid nke ụkwara nta Mycobacterium. Ọ na-enyere aka na nchọpụta nke ọrịa ụkwara nta Mycobacterium, ọ na-achọpụtakwa mkpụrụ ndụ ihe nketa mgbochi nke rifampicin na isoniazid, nke na-enyere aka ịghọta iguzogide ọgwụ nke ụkwara nta Mycobacterium nke onye ọrịa butere.

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Ngwa Nchọpụta HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid na Rifampicin (RIF), (INH) (Mgbaze Curve)

Ọmụmụ ihe gbasara ọrịa na-efe efe

Ọrịa ụkwara nta nke Mycobacterium, dịka Tubercle Bacillus (TB), bụ nje bacteria na-akpata ụkwara nta, ugbu a, ọgwụ ndị a na-ejikarị eme ihe bụ isoniazid, rifampicin na ethambutol, wdg.^[1]Agbanyeghị, n'ihi ojiji na-ezighi ezi nke ọgwụ mgbochi ụkwara nta na njirimara nke usoro mgbidi sel nke ụkwara nta mycobacterium n'onwe ya, ụkwara nta mycobacterium emepụtala iguzogide ọgwụ megide ọgwụ mgbochi ụkwara nta, ụdị dị oke ize ndụ bụ ụkwara nta na-eguzogide ọgwụ dị iche iche (MDR-TB), nke na-eguzogide ọgwụ abụọ kachasị dị irè ma dị irè, rifampicin na isoniazid.^[2].

Nsogbu nke iguzogide ọgwụ ụkwara nta dị na mba niile nke WHO nyochara. Iji nye atụmatụ ọgwụgwọ ziri ezi maka ndị ọrịa ụkwara nta, ọ dị mkpa ịchọpụta iguzogide ọgwụ mgbochi ụkwara nta, ọkachasị iguzogide rifampicin, nke aghọọla usoro nchọpụta nke WHO kwadoro na ọgwụgwọ ụkwara nta.^[3]Ọ bụ ezie na nchọpụta nke iguzogide rifampicin fọrọ nke nta ka ọ bụrụ otu ihe ahụ dị ka nchọpụta nke MDR-TB, naanị ịchọpụta iguzogide rifampicin na-eleghara ndị ọrịa nwere INH mono-resistant (nke na-ezo aka na iguzogide isoniazid mana ọ na-enwe mmetụta na rifampicin) na rifampicin mono-resistant (nsogbu na isoniazid mana ọ na-eguzogide rifampicin), nke nwere ike ibute ndị ọrịa na-enweta usoro ọgwụgwọ mbụ na-enweghị isi. Ya mere, nnwale iguzogide isoniazid na rifampicin bụ ihe kacha mkpa dị mkpa na mmemme njikwa DR-TB niile.^[⁴^].

Usoro Nkà na ụzụ

Nchekwa	≤-18℃
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ihe atụ	Ihe nlele Sputum, Omenala siri ike (LJ Medium), Omenala mmiri mmiri (MGIT Medium)
CV	<5.0%
LoD	LoD nke ngwa ahụ maka ịchọpụta ụkwara nta Mycobacterium bụ nje bacteria iri/mL;LoD nke ngwa maka ịchọpụta ụdị anụ ọhịa na ụdị mutant rifampicin bụ nje bacteria 150/mL; LoD nke ngwa maka ịchọpụta ụdị anụ ọhịa isoniazid na ụdị mutant bụ nje bacteria 200/mL.
Nkọwapụta	1) Enweghị mmeghachi omume cross mgbe ejiri ngwa ahụ chọpụta DNA genomic mmadụ (500ng), ụdị nje iku ume 28 ndị ọzọ, na ụdị mycobacteria na-abụghị ụkwara nta 29 (dịka egosiri na Tebụl 3).2) Enweghị mmeghachi omume cross mgbe ejiri ngwa ahụ chọpụta ebe mgbanwe nke mkpụrụ ndụ ihe nketa ndị ọzọ na-eguzogide ọgwụ nke rifampicin na ụkwara nta Mycobacterium nke na-adịghị emetụta isoniazid (dịka egosiri na Tebụl 4).3) Ihe ndị a na-ahụkarị na-egbochi ihe ndị a ga-anwale, dịka rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide (45mg/L), zanamivir (0.5mg/L), dexamethasone (20mg/L), enweghị mmetụta ọ bụla na nsonaazụ ule kit ahụ.
Ngwa Ndị Dị Mkpa	Sistemụ PCR SLAN-96P n'oge a na-ahụ anya (Hongshi Medical Technology Co., Ltd.), Sistemụ PCR Oge-Oge BioRad CFX96