Mycobacterium ụkwara nta Nucleic Acid na Rifampicin (RIF) ， Nguzogide (INH)

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A na-eji ngwa a maka nchọpụta qualitative in vitro nke Mycobacterium ụkwara nta DNA na sputum mmadụ, omenala siri ike (LJ Medium) na mmiri mmiri omenala (MGIT Medium), bronchial lavage fluid, na mutations na 507-533 amino acid codon region (81bp, rifampicin resistance determining region) nke rcopoBcicin na-eguzogide ọgwụ na mpaghara MycopoB. Mgbanwe ndị dị na saịtị ndị bụ isi nke Mycobacterium tuberculosis isoniazid resistance.Ọ na-enye aka na nchọpụta nke ọrịa ụkwara nta nke Mycobacterium, ọ na-achọpụtakwa mkpụrụ ndụ ihe nketa nke rifampicin na isoniazid, nke na-enyere aka ịghọta nguzogide ọgwụ nke Mycobacterium ụkwara nta nke onye ọrịa butere.

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HWTS-RT147 Mycobacterium ụkwara nta Nucleic Acid na Rifampicin(RIF), (INH) Nchọpụta Ngwa (Melting Curve)

Epidemiology

Mycobacterium ụkwara nta, obere oge dị ka Tubercle bacillus (TB), bụ nje nje na-akpata ụkwara nta, ma ugbu a, ọgwụ ndị a na-ejikarị alụso ọrịa ọgụ nke mbụ na-agụnye isoniazid, rifampicin na ethambutol, wdg.^[1]. Otú ọ dị, n'ihi na-ezighị ezi ojiji nke mgbochi ụkwara nta ọgwụ na e ji mara cell mgbidi Ọdịdị nke mycobacterium ụkwara nta n'onwe ya, mycobacterium ụkwara nta emepụtawo ọgwụ iguzogide mgbochi ụkwara nta ọgwụ, na a karịsịa dị ize ndụ ụdị bụ multidrug-eguzogide ụkwara nta (MDR-TB), nke na-eguzogide ọgwụ abụọ kasị nkịtị na irè ọgwụ, rifampicin na.^[2].

Nsogbu nke ụkwara nta na-eguzogide ọgwụ dị na mba niile nke WHO nyochara. Iji wepụta usoro ọgwụgwọ ziri ezi maka ndị ọrịa ụkwara nta, ọ dị mkpa ịchọpụta iguzogide ọgwụ mgbochi ụkwara nta, ọkachasị nguzogide rifampicin, nke ghọrọ usoro nyocha nke WHO tụrụ aro na ọgwụgwọ ụkwara nta.^[3]. Ọ bụ ezie na nchọpụta nke nguzogide rifampicin fọrọ nke nta ka ọ bụrụ nchọpụta nke MDR-TB, nanị ịchọpụta nguzogide rifampicin na-eleghara ndị ọrịa nwere INH na-eguzogide mono-eguzogide (na-ezo aka na-eguzogide isoniazid ma na-enwe mmetụta nke rifampicin) na mono-eguzogide rifampicin (nhụta nke isoniazid ma iguzogide rifampicin), nke nwere ike iduga ndị ọrịa na-enweghị ọgwụgwọ mbụ. Ya mere, ule nguzogide isoniazid na rifampicin bụ ihe kacha mkpa achọrọ na mmemme njikwa DR-TB niile^[⁴^].

Nka nka

Nchekwa	≤-18℃
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ụdị	Sample Sputum, Omenala siri ike (Ọkara LJ), Omenala Liquid (Ọkara MGIT)
CV	<5.0%
LoD	LoD nke ngwa maka ịchọpụta ụkwara nta nke Mycobacterium bụ nje bacteria 10 / ml;LoD nke kit maka ịchọpụta ụdị anụ ọhịa rifampicin na ụdị mutant bụ 150 bacteria / ml; LoD nke kit maka ịchọpụta ụdị anụ ọhịa isoniazid na ụdị mutant bụ 200 bacteria/ml.
Nkọwapụta	1) Enweghị mmeghachi omume ọ bụla mgbe ị na-eji ngwa ahụ chọpụta DNA genomic nke mmadụ (500ng), ụdị 28 ndị ọzọ nke iku ume iku ume, na ụdị 29 nke mycobacteria na-abụghị tuberculous (dị ka e gosiri na Tebụl 3).2) Enweghị mmeghachi omume ọ bụla mgbe ị na-eji ngwa ahụ chọpụta ebe mgbanwe nke mkpụrụ ndụ ihe nketa rifampicin ndị ọzọ na-eguzogide ọgwụ na isoniazid kókó Mycobacterium ụkwara nta (dị ka e gosiri na Tebụl 4).3) Ihe ndị na-etinyekarị aka na nlele a ga-anwale, dị ka rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide), (45mg/L. ọgwụ (20mg/L), enweghị mmetụta na nsonaazụ ule kit.
Ngwa ndị ọdabara	SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 ezigbo oge PCR sistemụ