Mycoplasma pneumoniae (MP)
Aha ngwaahịa
HWTS-RT024 Mycoplasma Pneumoniae (MP) Nchọpụta Nucleic Acid (Fluorescence PCR)
Epidemiology
Mycoplasma pneumoniae (MP) bụ ụdị obere prokaryotic microorganism, nke dị n'etiti nje bacteria na nje, nwere nhazi cell ma enweghị mgbidi cell.MP na-ebutekarị ọrịa iku ume mmadụ, ọkachasị ụmụaka na ndị ntorobịa.Ọ nwere ike ibute mycoplasma oyi baa mmadụ, ọrịa akụkụ iku ume ụmụaka na oyi baa na-adịghị ahụkebe.Ihe ngosi ụlọ ọgwụ dị iche iche, ọtụtụ n'ime ha bụ ụkwara siri ike, ahụ ọkụ, oyi, isi ọwụwa, akpịrị akpịrị.Ọrịa elu akụkụ iku ume na oyi baa na bronchial bụ ihe a na-ahụkarị.Ụfọdụ ndị ọrịa nwere ike ịmalite site na ọrịa akụkụ iku ume elu ruo na oyi baa siri ike, nnukwu nsogbu iku ume na ọnwụ nwere ike ime.
Ọwa
| FAM | Mycoplasma pneumonia |
| VIC/HEX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Sputum, Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 200 mbipụta/ml |
| Nkọwapụta | a) Cross reactivity: ọ dịghị cross reactivity na Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Klebsiella pneumoniae, Staphylococcus, mycoccusbacteriosis, mycoccus. eruginosa, Acinetobacter baumannii, nje influenza A , Influenza B nje, Parainfluenza nje ụdị I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial nje na mmadụ genomic nucleic acid. b) Ikike mgbochi nnyonye anya: ọ dịghị nnyonye anya mgbe a nwalere ihe ndị na-etinye aka na mkpokọta ndị a: hemoglobin (50mg / L), bilirubin (20mg / dL), mucin (60mg / ml), 10% (v / v) ọbara mmadụ, levofloxacin (10μg / ml), moxifloxacin (0.1g / L), gemifloxacin (80μg / ml), azithromycin (1mg / ml), clarithromycin (125μg / ml), erythromycin (0.5g / L), doxycycline (50mg). /L), minocycline (0.1g/L). |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo QuantStudio®Sistemụ PCR 5 ezigbo oge SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®Sistemụ PCR 480 ezigbo oge Sistemụ nchọpụta PCR nke LineGene 9600 Plus (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 ezigbo oge PCR sistemụ BioRad CFX Opus 96 PCR ezigbo oge |
Usoro ọrụ
(1) Ihe nlele sputum
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke nwere ike iji na Macro & Micro-Test). Akpaaka Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tinye 200µL nke saline nkịtị n'ọsọ mmiri esichara.Ekwesịrị ịme mmịpụta na-esote dịka ntuziaka maka ojiji.Olu elution akwadoro bụ 80µL. A na-atụ aro reagent mmịpụta: Nucleic Acid Extraction or Purification Reagent (YDP315-R).Ekwesịrị ịme mmịpụta ahụ nke ọma dịka ntuziaka maka ojiji.Olu elution akwadoro bụ 60µL.
(2) Oropharyngeal swab
Na-atụ aro mmịpụta reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke nwere ike iji na Macro & Micro-Test). Akpaaka Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekwesịrị ịme mmịpụta dịka ntuziaka maka ojiji.Olu mmịpụta akwadoro nke sample bụ 200µL, na olu elution akwadoro bụ 80µL. akwadoro mmịpụta reagent: QIAamp Viral RNA Mini Kit (52904) ma ọ bụ Nucleic Acid Extraction or Purification Reagent (YDP315-R).Ekwesịrị ịme mmịpụta ahụ nke ọma dịka ntuziaka maka ojiji.Oke mmịpụta akwadoro nke nlele bụ 140µL, na olu elution akwadoro bụ 60µL.
