Ọrịa oyi baa nke Mycoplasma (MP)
Aha ngwaahịa
Ngwa Nchọpụta Asịd Nucleic (PCR Fluorescence)
Ọmụmụ ihe gbasara ọrịa na-efe efe
Mycoplasma pneumoniae (MP) bụ ụdị obere nje prokaryotic, nke dị n'etiti nje bacteria na nje virus, nke nwere nhazi sel mana enweghị mgbidi sel. MP na-ebutekarị ọrịa iku ume mmadụ, ọkachasị n'ime ụmụaka na ndị ntorobịa. Ọ nwere ike ibute ọrịa mycoplasma pneumonia mmadụ, ọrịa iku ume ụmụaka na ọrịa pneumonia na-adịghị ahụkebe. Ihe mgbaàmà ahụike dị iche iche, ọtụtụ n'ime ha bụ ụkwara siri ike, ahụ ọkụ, oyi, isi ọwụwa, akpịrị mgbu. Ọrịa iku ume elu na ọrịa pneumonia bronchial bụ ihe a na-ahụkarị. Ụfọdụ ndị ọrịa nwere ike ịmalite site na ọrịa iku ume elu ruo na pneumonia siri ike, nnukwu nsogbu iku ume na ọnwụ nwere ike ime.
Ọwa
| FAM | Ọrịa pneumonia nke Mycoplasma |
| VIC/HEX | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | Sputum, swab oropharyngeal |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Mbipụta 200/mL |
| Nkọwapụta | a) Mmeghachi omume n'ụzọ dị iche iche: enweghị mmeghachi omume n'ụzọ dị iche iche na Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacterium tuberculosis, Legionella pneumophila, Pseudomonas aeruginosa, Acinetobacter baumannii, Influenza A virus, Influenza B virus, Parainfluenza virus type I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial virus na human genomic nucleic acid. b) Ike igbochi ihe mgbochi: enweghị ihe mgbochi mgbe a nwalere ihe ndị na-egbochi ihe ndị a site na njupụta ndị a: hemoglobin (50mg/L), bilirubin (20mg/dL), mucin (60mg/mL), 10% (v/v) ọbara mmadụ, levofloxacin (10μg/mL), moxifloxacin (0.1g/L), gemifloxacin (80μg/mL), azithromycin (1mg/mL), clarithromycin (125μg/mL), erythromycin (0.5g/L), doxycycline (50mg/L), minocycline (0.1g/L). |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR Ngwa-ngwa Ngwa 7500 nke Ejiri Ngwa Ngwa QuantStudio®Sistemụ PCR 5 n'oge a na-arụ ọrụ Sistemụ PCR SLAN-96P nke Oge-N'ezie (Hongshi Medical Technology Co., Ltd.) LightCycler®Sistemụ PCR oge 480 Sistemụ Nchọpụta PCR nke LineGene 9600 Plus n'oge dị adị (FQD-96A, teknụzụ Hangzhou Bioer) MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya (Suzhou Molarray Co., Ltd.) Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR Oge Nta nke BioRad CFX Opus 96 |
Usoro Ọrụ
(1) Ihe nlele mmiri
Ihe na-eme ka a na-ewepụta ihe: Ngwakọta DNA/RNA nke Macro & Micro-Test Viral (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke enwere ike iji Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tinye 200µL nke saline nkịtị na ihe na-eme ka a na-emepụta ihe. A ga-eme ka ihe na-esote ya dịka ntuziaka maka ojiji si dị. Olu ihe na-eme ka ihe dị mma bụ 80µL. Ihe na-eme ka ihe dị mma maka mwepụ ihe: Ngwakọta Nucleic Acid ma ọ bụ ihe na-eme ka ihe dị ọcha (YDP315-R). A ga-eme ka ihe ahụ dị mma dịka ntuziaka maka ojiji si dị. Olu ihe na-eme ka ihe dị mma maka iwepụ ihe dị mma bụ 60µL.
(2) Swab oropharyngeal
Ngwa mwepụta akwadoro: Ngwakọta Macro & Micro-Test Viral DNA/RNA (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (nke enwere ike iji Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekwesịrị ime mwepụta ahụ dịka ntuziaka maka ojiji si dị. Olu mwepụta akwadoro nke ihe nlele bụ 200µL, olu mwepụta akwadoro bụ 80µL. Ngwa mwepụta akwadoro akwadoro: QIAamp Viral RNA Mini Kit (52904) ma ọ bụ Nucleic Acid Extraction or Purification Reagent (YDP315-R). Ekwesịrị ime mwepụta ahụ kpọmkwem dịka ntuziaka maka ojiji si dị. Olu a tụrụ aro maka mwepụta nke ihe nlele bụ 140µL, olu a tụrụ aro maka iwepụ ihe nlele bụ 60µL.
