Ihe Ndị Na-akpata Ọrịa Iku Oku Ejikọtara
Aha ngwaahịa
Ngwa Nchọpụta Agwakọtara nke HWTS-RT158A nke Ọrịa Iku Ume (Fluorescence PCR)
Asambodo
CE
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa Coronavirus nke afọ 2019, nke a na-akpọ "Ọrịa Coronavirus nke afọ 2019"'COVID 19', na-ezo aka na oyi baa nke ọrịa 2019-nCoV kpatara. 2019-nCoV bụ coronavirus nke sitere na β genus. COVID-19 bụ ọrịa na-efe efe ngwa ngwa nke na-efe efe n'ikuku, ndị mmadụ na-enwekarị ike ibute ya. Ugbu a, isi iyi nke ọrịa bụ ndị ọrịa butere na 2019-nCoV, ndị nwere ihe mgbaàmà nwekwara ike ịbụ isi iyi nke ọrịa ahụ. Dabere na nyocha ọrịa na-efe efe ugbu a, oge mmalite nke ọrịa ahụ bụ ụbọchị 1-14, ọkachasị ụbọchị 3-7. Ahụ ọkụ, ụkwara kpọrọ nkụ na ike ọgwụgwụ bụ ihe ndị bụ isi na-egosi. Ụfọdụ ndị ọrịa nwere ihe mgbaàmà dịka mkpọchi imi, imi mmiri, akpịrị mgbu, myalgia na afọ ọsịsa, wdg.
Influenza, nke a na-akpọkarị "influenza", bụ ọrịa na-efe efe nke nje influenza na-akpata. Ọ na-efe efe nke ukwuu. A na-ebutekarị ya site na ụkwara na ịsụ ude. Ọ na-agbasakarị n'oge opupu ihe ubi na oyi. A na-ekewa nje influenza n'ime ụdị atọ nke influenza A (IFV A), influenza B (IFV B), na Influenza C (IFV C) nke nje na-arapara arapara, na-akpata ọrịa mmadụ karịsịa maka nje influenza A na B, ọ bụ nje RNA nke nwere otu eriri, nke kewara ekewa. Influenza A bụ ọrịa iku ume dị oke njọ, gụnyere H1N1, H3N2 na ụdị ndị ọzọ, nke na-adịkarị mfe ịgbanwe ma gbasaa n'ụwa niile. "Shift" na-ezo aka na mgbanwe nke nje influenza A, nke na-ebute mpụta nke "ụdị" nje ọhụrụ. A na-ekewa nje influenza B n'ime usoro abụọ, Yamagata na Victoria. Nje influenza B nwere naanị antigenic drift, ọ na-ezere nlekota na mwepụ nke sistemu ahụ ji alụso ọrịa ọgụ site na mgbanwe ya. Agbanyeghị, ọsọ evolushọn nke nje influenza B dị nwayọ karịa nke nje influenza A mmadụ. Ọrịa Influenza B nwekwara ike ibute ọrịa iku ume mmadụ ma bute ọrịa na-efe efe.
Nje virus nke na-eme ka mmadụ ghara iku ume (RSV) bụ nje RNA, nke sitere n'ezinụlọ paramyxoviridae. A na-ebufe ya site na ntapụ ikuku na kọntaktị chiri anya, ọ bụkwa isi ihe na-akpata ọrịa akụkụ iku ume ala n'ime ụmụ ọhụrụ. Ụmụ ọhụrụ nwere RSV nwere ike ibute nnukwu bronchiolitis na pneumonia, nke metụtara ụkwara ume ọkụ n'ime ụmụaka. Ụmụ ọhụrụ nwere nnukwu mgbaàmà, gụnyere oke ahụ ọkụ, rhinitis, pharyngitis na laryngitis, wee bụrụ bronchiolitis na pneumonia. Ụmụaka ole na ole na-arịa ọrịa nwere ike inwe mgbagwoju anya na otitis media, pleurisy na myocarditis, wdg. Ọrịa akụkụ iku ume elu bụ isi ihe mgbaàmà nke ọrịa n'ime ndị okenye na ụmụaka toro eto.
Ọwa
| FAM | SARS-CoV-2 |
| VIC (HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| Quasar 705 | Njikwa Dị n'ime |
Usoro Nkà na ụzụ
| Nchekwa | -18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | Swab nke oropharyngeal |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Mpempe/mL Ọrịa Influenza A/Nje Influenza B/Nje syncytial nke iku ume: 500Isi/mL |
| Nkọwapụta | a) Nsonaazụ mmeghachi omume na-egosi na enweghị mmeghachi omume mmegharị n'etiti kit na nje virus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, ụdị nje parainfluenza 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, nje metapneumovirus mmadụ, enterovirus A, B, C, D, nje epstein-barr, nje measles, nje cytomegalo mmadụ, rotavirus, norovirus, nje parotitis, nje varicella-zoster, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci na ọhụrụ cryptococcus na human genomic nucleic acid. b) Ike igbochi ihe mgbochi: select mucin (60mg/mL), 10% (v/v) nke ọbara na phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (gụnyere ihe nchekwa) (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) na tobramycin (0.6mg/mL) maka nnwale nnyonye anya, nsonaazụ ya na-egosikwa na ihe ndị na-egbochi ndị nwere oke ahụ e kwuru n'elu enweghị mmeghachi omume nnyonye anya na nsonaazụ nnwale nke nje. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-Oge BioRad CFX96 Sistemụ PCR nke Rotor-Gene Q 5plex HRM n'oge dị adị |
