Ejikọtara Ọrịa iku ume
Aha ngwaahịa
Ngwa ihe nchọpụta agwakọta HWTS-RT158A iku ume (Fluorescence PCR)
Asambodo
CE
Epidemiology
Ọrịa Corona Virus 2019, nke a na-akpọ'COVID 19', na-ezo aka na oyi baa nke ọrịa 2019-nCoV kpatara. 2019-nCoV bụ coronavirus bụ nke β genus. COVID-19 bụ ọrịa na-efe efe na-efe efe na-efe efe na-efe efe, ndị mmadụ na-enwekarịkwa ike. Ka ọ dị ugbu a, isi mmalite nke ọrịa bụ ndị ọrịa butere site na 2019-nCoV, na ndị butere ọrịa asymptomatic nwekwara ike bụrụ isi mmalite nke ọrịa. Dabere na nyocha ọrịa ọrịa ugbu a, oge nnabata bụ ụbọchị 1-14, ọkachasị ụbọchị 3-7. Ahụ ọkụ, ụkwara akọrọ na ike ọgwụgwụ bụ isi ihe ngosi. Ndị ọrịa ole na ole nwere ihe mgbaàmà dị ka imi imi, imi imi, akpịrị akpịrị, myalgia na afọ ọsịsa, wdg.
Influenza, nke a na-akpọkarị "flu", bụ nnukwu ọrịa na-efe efe nke iku ume nke nje influenza kpatara. Ọ na-efe efe nke ukwuu. A na-ebutekarị ya site na ụkwara na imi. Ọ na-apụtakarị na mmiri na oyi. A na-ekewa nje virus influenza n'ime influenza A (IFV A), influenza B (IFV B), na influenza C (IFV C) ụdị atọ, ha niile bụ nje virus na-egbuke egbuke, na-ebute ọrịa mmadụ karịsịa maka nje nje A na B, ọ bụ nje RNA nwere otu eriri, nke kewara ekewa. Influenza A bụ nnukwu ọrịa iku ume, gụnyere H1N1, H3N2 na ụdị ndị ọzọ, nke na-enwekarị mmụgharị na ntiwapụ n'ụwa nile. "Shift" na-ezo aka na ngbanwe nke nje influenza A, na-ebute mpụta nke nje ọhụrụ "subtype". A na-ekewa nje virus influenza B ụzọ abụọ, Yamagata na Victoria. Nje virus influenza B nwere naanị ihe mgbochi antigenic, ọ na-ezere nleba anya na mkpochapụ nke sistemu ahụ ji alụso ọrịa ọgụ site na ngbanwe ya. Otú ọ dị, evolushọn ọsọ nke nje influenza B dị nwayọọ karịa nke nje influenza A mmadụ. Nje virus influenza B nwekwara ike ibute ọrịa iku ume mmadụ ma bute ọrịa na-efe efe.
Nje virus syncytial iku ume (RSV) bụ nje RNA, nke ezinụlọ paramyxoviridae. A na-ebunye ya site na ụmụ irighiri mmiri ikuku na nso nso a na ọ bụ isi ihe na-akpata ọrịa iku ume n'ime ụmụ ọhụrụ. Ụmụaka nwere ọrịa RSV nwere ike ịmalite bronchiolitis siri ike na oyi baa, nke metụtara ụkwara ume ọkụ na ụmụaka. Ụmụaka na-enwe mgbaàmà siri ike, gụnyere nnukwu ahụ ọkụ, rhinitis, pharyngitis na laryngitis, na mgbe ahụ bronchiolitis na oyi baa. Ole na ole ụmụaka na-arịa ọrịa nwere ike mgbagwoju anya na otitis media, pleurisy na myocarditis, wdg. elu akụkụ okuku ume na tract ọrịa bụ isi ihe mgbaàmà nke ọrịa na ndị okenye na ndị okenye ụmụaka.
Ọwa
| FAM | SARS-CoV-2 |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| Afọ 705 | Njikwa ime |
Nka nka
| Nchekwa | -18 ℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300 oyiri/ml Influenza A virus/Influenza B nje/Nje virus syncytial iku ume: 500Copy/mL |
| Nkọwapụta | a) Cross-reactivity results na-egosi na ọ dịghị cross mmeghachi omume n'etiti kit na mmadụ coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza nje ụdị 1, 2, 3, rhinovirus A, B, diamyvirus chulamoni, C enterovirus A, B, C, D, epstein-barr virus, measles virus, mmadụ cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus, pneumonia, streptococcus, pneumonia. oyi baa, mycobacterium ụkwara nta, anwụrụ ọkụ aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci na nwa amụrụ ọhụrụ cryptococcus na mmadụ genomic nucleic acid. b) Mgbochi nnyonye anya: họrọ mucin (60mg / ml), 10% (v / v) nke ọbara na phenylephrine (2mg / ml), oxymetazoline (2mg / ml), sodium chloride (gụnyere preservatives) (20mg / mL), beclomethasone (20mg / mL), 20mgalisone (20mg / ml), dexamnisone. (20μg / ml), triamcinolone acetonide (2mg / ml), budesonide (2mg / ml), mometasone (2mg / ml), fluticasone (2mg / ml), histamine hydrochloride (5mg / ml), alpha interferon (800IU / ml), zanamivir (20mg/mL) , ovarim ribavirin. (60ng / ml), peramivir (1mg / ml), lopinavir (500mg / ml), ritonavir (60mg / mL), mupirocin (20mg / ml), azithromycin (1mg / ml), ceftriaxone (40μg / ml), meropenem (200mg / ml / ml), 10μcin na levocin. (0.6mg / mL) maka nyocha nnyonye anya, nsonaazụ ya na-egosi na ihe ndị na-etinye aka na ntinye aka ndị a kpọtụrụ aha n'elu enweghị mmeghachi omume nnyonye anya na nsonaazụ ule nke nje. |
| Ngwa ndị ọdabara | BioRad CFX96 ezigbo oge PCR sistemụ Rotor-Gene Q 5plex HRM Platform ezigbo oge PCR Sistemu |
