Ejikọtara Ọrịa iku ume

Nkọwa dị mkpirikpi:

A na-eji ngwa a maka nchọpụta qualitative nke ọrịa na-eku ume na nucleic acid ewepụtara n'ụdị swab oropharyngeal mmadụ.

A na-eji ihe atụ a maka nchọpụta qualitative nke 2019-nCoV, nje influenza A, nje influenza B na nje virus syncytial nucleic acid n'ime ihe nlele swab oropharyngeal mmadụ.

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Ngwa ihe nchọpụta agwakọta HWTS-RT158A iku ume (Fluorescence PCR)

Asambodo

Epidemiology

Ọrịa Corona Virus 2019, nke a na-akpọ'COVID 19', na-ezo aka na oyi baa nke ọrịa 2019-nCoV kpatara.2019-nCoV bụ coronavirus bụ nke β genus.COVID-19 bụ ọrịa na-efe efe na-efe efe na-efe efe na-efe efe, ndị mmadụ na-enwekarịkwa ike.Ka ọ dị ugbu a, isi mmalite nke ọrịa bụ ndị ọrịa butere site na 2019-nCoV, yana ndị butere ọrịa asymptomatic nwekwara ike bụrụ isi mmalite nke ọrịa.Dabere na nyocha ọrịa ọrịa ugbu a, oge nnabata bụ ụbọchị 1-14, ọkachasị ụbọchị 3-7.Ahụ ọkụ, ụkwara akọrọ na ike ọgwụgwụ bụ isi ihe ngosi.Ndị ọrịa ole na ole nwere ihe mgbaàmà dị ka imi imi, imi imi, akpịrị akpịrị, myalgia na afọ ọsịsa, wdg.

Influenza, nke a na-akpọkarị "flu", bụ nnukwu ọrịa na-efe efe nke iku ume nke nje influenza kpatara.Ọ na-efe efe nke ukwuu.A na-ebutekarị ya site na ụkwara na imi.Ọ na-apụtakarị na mmiri na oyi.A na-ekewa nje virus influenza n'ime influenza A (IFV A), influenza B (IFV B), na influenza C (IFV C) ụdị atọ, ha niile bụ nje virus na-egbuke egbuke, na-ebute ọrịa mmadụ karịsịa maka nje influenza A na B, ọ bụ otu. - stranded, nke RNA nje.Influenza A bụ nnukwu ọrịa iku ume, gụnyere H1N1, H3N2 na ụdị ndị ọzọ, nke na-enwekarị mmụgharị na ntiwapụ n'ụwa nile."Shift" na-ezo aka na ngbanwe nke nje influenza A, na-ebute mpụta nke nje ọhụrụ "subtype".A na-ekewa nje virus influenza B ụzọ abụọ, Yamagata na Victoria.Nje virus influenza B nwere naanị ihe mgbochi antigenic, ọ na-ezere nleba anya na mkpochapụ nke sistemu ahụ ji alụso ọrịa ọgụ site na ngbanwe ya.Otú ọ dị, evolushọn ọsọ nke nje influenza B dị nwayọọ karịa nke nje influenza A mmadụ.Influenza B nwekwara ike bute ọrịa iku ume mmadụ ma bute ọrịa na-efe efe.

Nje virus syncytial iku ume (RSV) bụ nje RNA, nke sitere na ezinụlọ paramyxoviridae.A na-ebute ya site na ụmụ irighiri mmiri ikuku na nso nso a na ọ bụ isi ihe na-akpata ọrịa iku ume n'ime ụmụ ọhụrụ.Ụmụaka nwere ọrịa RSV nwere ike ịmalite bronchiolitis siri ike na oyi baa, nke metụtara ụkwara ume ọkụ na ụmụaka.Ụmụaka na-enwe mgbaàmà siri ike, gụnyere nnukwu ahụ ọkụ, rhinitis, pharyngitis na laryngitis, na mgbe ahụ bronchiolitis na oyi baa.Ole na ole ụmụaka na-arịa ọrịa nwere ike mgbagwoju anya na otitis media, pleurisy na myocarditis, wdg. elu akụkụ okuku ume na tract ọrịa bụ isi ihe mgbaàmà nke ọrịa na ndị okenye na ndị okenye ụmụaka.

Ọwa

FAM	SARS-CoV-2
VIC(HEX)	RSV
CY5	IFV A
ROX	IFV B
Afọ 705	Njikwa ime

Nka nka

Nchekwa	-18 ℃
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ụdị	Oropharyngeal swab
Ct	≤38
LoD	2019-nCoV: 300 oyiri/ml Influenza A virus/Influenza B nje/Nje virus syncytial iku ume: 500Copy/mL
Nkọwapụta	a) Nsonaazụ mmeghachi omume na-egosi na enweghị mmeghachi omume n'etiti ngwa ahụ na coronavirus mmadụ SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, ụdị nje parainfluenza 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, mmadụ metapneumovirus, enterovirus A, B, C, D, epstein-barr virus, measles virus, mmadụ cytomegalo virus, rotavirus, norovirus, parotitis nje, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium ụkwara nta, anwụrụ ọkụ aspergillus, candida albicans, candida glabrata, pnemmụ mmadụ kriptococcus n'ime ọhụrụ na acid n'ime anụ ahụ. b) Ikike mgbochi nnyonye anya: họrọ mucin (60mg/mL), 10% (v/v) nke ọbara na phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (gụnyere preservatives) (20mg/mL) , beclomethasone (20mg / ml), dexamethasone (20mg / ml), flunisolide (20μg / ml), triamcinolone acetonide (2mg / ml), budesonide (2mg / ml), mometasone (2mg / ml), fluticasone (2mg / ml). , histamine hydrochloride (5mg/mL), alpha interferon (800IU / ml), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL). Ritonavir (60mg / ml), mupirocin (20mg / ml), azithromycin (1mg / ml), ceftriaxone (40μg / ml), meropenem (200mg / ml), levofloxacin (10μg / ml) na tobramycin (0.6mg/mL). ) maka nyocha nnyonye anya, na nsonaazụ ya na-egosi na ihe ndị na-etinye aka na ntinye uche ndị a kpọtụrụ aha n'elu enweghị mmeghachi omume nnyonye anya na nsonaazụ ule nke nje.
Ngwa ndị ọdabara	BioRad CFX96 ezigbo oge PCR sistemụ Rotor-Gene Q 5plex HRM Platform ezigbo oge PCR Sistemu