Ọrịa Urogenital asaa

Nkọwa dị mkpirikpi:

A na-eji ngwa a maka nchọpụta qualitative chlamydia trachomatis (CT), neisseria gonorrhoeae (NG) na mycoplasma genitalium (MG), mycoplasma hominis (MH), herpes simplex virus ụdị 2 (HSV2), ureaplasma parvum (UP) na ureaplasma urealyticum. (UU) nucleic acids na nwoke urethral swabs na nwanyị cervical swab sample in vitro, maka enyemaka iji chọpụta na ọgwụgwọ nke ndị ọrịa nwere ọrịa genitourinary tract.

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Aha ngwaahịa

HWTS-UR017A Ngwa nchọpụta Urogenital Pathogen Nucleic Acid asaa (mgbaze agbaze)

Epidemiology

Ọrịa ndị a na-ebute site ná mmekọahụ (STD) ka bụ otu n'ime ihe ndị dị mkpa na-eyi nchebe ahụike ọha na eze gburugburu ụwa, nke nwere ike ibute enweghị ọmụmụ, ịmụ nwa, etuto na nsogbu dị iche iche dị egwu.Ọrịa ndị a na-ahụkarị gụnyere chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma genitalium, mycoplasma hominis, herpes simplex virus ụdị 2, ureaplasma parvum, na ureaplasma urealyticum.

Ọwa

FAM	CT na NG
HEX	MG, MH na HSV2
ROX	Njikwa ime

Nka nka

Nchekwa	≤-18℃
Ndụ nchekwa	Ọnwa iri na abụọ
Ụdị ụdị	secretions urethra Ihe nzuzo nke cervical
Tt	≤28
CV	≤5.0%
LoD	CT: 500 oyiri/ml NG:400 oyiri/ml MG: 1000 oyiri/ml MH: 1000 oyiri/ml HSV2:400 oyiri/ml Elu: 500 oyiri/ml UU: 500 oyiri/ml
Nkọwapụta	Nwalee nje ndị butere ọrịa na mpụga nso nso nke ngwa ule, dị ka treponema pallidum, candida albicans, trichomonas vaginalis, staphylococcus epidermidis, escherichia coli, gardnerella vaginalis, adenovirus, cytomegalovirus, beta Streptococcus, HIV, lactobacillus case.Ma ọ dịghị cross-reactivity. Ikike mgbochi nnyonye anya: 0.2 mg/mL bilirubin, imi cervical, 10⁶mkpụrụ ndụ ọbara ọcha / mL, 60 mg / ml mucin, ọbara zuru oke, semen, ọgwụ antifungal a na-ejikarị (200 mg / ml levofloxacin, 300 mg / ml erythromycin, 500 mg / ml penicillin, 300mg/mL azithromycin, 10% Jieryin lotion. , 5% Fuyanjie ude mmiri) etinyela aka na ngwa ahụ.
Ngwa ndị ọdabara	Sistemụ PCR SLAN-96P ezigbo oge Sistemụ PCR Real-Time LightCycler®480

Usoro ọrụ

Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).

A) Usoro ntuziaka: Were tube centrifuge na-enweghị 1.5mL DNA ma tinye 200μL nke nlele a ga-anwale.Ekwesịrị ịwepụta usoro ndị na-esote n'ụzọ siri ike dabere na IFU.Oke elution akwadoro bụ 80μL.

B) Usoro akpaaka: Were ngwa mmịpụta eburula ụzọ tinye, gbakwunye 200 μL nke ihe nlele a ga-anwale n'ime ebe olulu mmiri dabara adaba, a ga-ewepụtakwa usoro ndị na-esote n'ụzọ siri ike dabere na IFU.

Nke gara aga: Mycoplasma Hominis

Osote: Chlamydia Trachomatis akpọnwụwo akpọnwụrụ

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