Enterococcus na-eguzogide ọgwụ na mkpụrụ ndụ ihe nketa na-eguzogide ọgwụ ọjọọ nke Vancomycin
Aha ngwaahịa
Ngwa Nchọpụta Mkpụrụ Ndụ Ihe Ndị Na-eguzogide Ọgwụ na Enterococcus Na-eguzogide Ọgwụ nke HWTS-OT090-Vancomycin (Fluorescence PCR)
Ọmụmụ ihe gbasara ọrịa na-efe efe
A makwaara iguzogide ọgwụ dịka iguzogide ọgwụ, nke pụtara iguzogide nje bacteria megide ọrụ nke ọgwụ nje. Ozugbo iguzogide ọgwụ mere, mmetụta chemotherapy nke ọgwụ ga-ebelata nke ukwuu. A na-ekewa iguzogide ọgwụ n'ime iguzogide n'ime na iguzogide enwetara. A na-ekpebi iguzogide n'ime site na mkpụrụ ndụ ihe nketa chromosomal nke nje bacteria, nke a na-agafe site n'ọgbọ ruo n'ọgbọ, ọ gaghị agbanwekwa. Mgbochi enwetara bụ n'ihi na mgbe ha metụrụ ọgwụ nje aka, nje bacteria na-agbanwe ụzọ metabolic nke ha ka ọgwụ nje ghara igbu ha.
Mkpụrụ ndụ ihe nketa ndị na-eguzogide ọgwụ vancomycin bụ́ VanA na VanB bụ ndị a na-enweta site n'igbochi ọgwụ, nke VanA na-egosi oke iguzogide ọgwụ vancomycin na teicoplanin, VanB na-egosi ọkwa dị iche iche nke iguzogide ọgwụ vancomycin, ọ na-emekwa ka teicoplanin nwee mmetụta. A na-ejikarị Vancomycin agwọ ọrịa nje bacteria Gram-positive, mana n'ihi mpụta nke enterococci na-eguzogide ọgwụ vancomycin (VRE), karịsịa enterococcus faecalis na enterococcus faecium, nke karịrị 90%, o wetawo nnukwu ihe ịma aka ọhụrụ na ọgwụgwọ ahụike. Ugbu a, enweghị ọgwụ nje kpọmkwem maka ọgwụgwọ VRE. Ihe ọzọkwa bụ na VRE nwekwara ike ibufe mkpụrụ ndụ ihe nketa ndị na-eguzogide ọgwụ nye enterococci ndị ọzọ ma ọ bụ nje bacteria Gram-positive ndị ọzọ.
Ọwa
| FAM | Enterococci na-eguzogide Vancomycin (VRE): Enterococcus faecalis na Enterococcus faecium |
| VIC/HEX | Njikwa Dị n'ime |
| CY5 | mkpụrụ ndụ ihe nketa na-eguzogide vancomycin VanB |
| ROX | mkpụrụ ndụ ihe nketa na-eguzogide vancomycin VanA |
Usoro Nkà na ụzụ
| Nchekwa | Mmiri mmiri: ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ihe atụ | afụ, ọbara, mmamịrị ma ọ bụ ezigbo eriri afọ |
| CV | ≤5.0% |
| Ct | ≤36 |
| LoD | 103CFU/mL |
| Nkọwapụta | Enweghị mmeghachi omume na nje ndị ọzọ na-akpata iku ume dịka klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, streptococcus pneumoniae, neisseria meningitidis, staphylococcus aureus, klebsiella oxytoca, haemophilus influenzae, A. junii, A. haemolyticus, legionella pneumophila, escherichia coli, pseudomonas fluorescens, candida albicans, chlamydia pneumoniae, respiratory adenovirus, ma ọ bụ ihe nlele nwere mkpụrụ ndụ ihe nketa ndị ọzọ na-eguzogide ọgwụ CTX, mecA, SME, Ihe nlele nke SHV na TEM. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500 etinyere Sistemụ PCR SLAN-96P Oge-N'ezie Sistemụ PCR oge-oge LightCycler®480 |
Usoro Ọrụ
Ihe ndị a tụrụ aro maka mwepụta: Ngwa DNA Genomic na Macro & Micro-Test (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
