Ụdị Ọrịa Iku Oku Iri Abụọ na Acid Nucleic Agwakọtara
Aha ngwaahịa
Ụdị HWTS-RT160 -29 nke Ọrịa Iku Oku Ngwa Nchọpụta Nucleic Acid Agwakọtara
Ọmụmụ ihe gbasara ọrịa na-efe efe
Ọrịa iku ume bụ ọrịa kachasị na ụmụ mmadụ, nke nwere ike ime n'ụdị nwoke na nwanyị, afọ na mpaghara ọ bụla. Ọ bụ otu n'ime ihe ndị na-akpata ọrịa na ọnwụ n'etiti ndị mmadụ n'ụwa niile[1]. Ọrịa iku ume a na-ahụkarị gụnyere coronavirus ọhụrụ, nje Influenza A, nje Influenza B, nje Respiratory syncytial, Adenovirus, nje metapneumovirus mmadụ, rhinovirus, ụdị nje Parainfluenza I/II/III, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, na Streptococcus pneumoniae, wdg[2,3]. Ihe mgbaàmà na ihe ịrịba ama nke ọrịa iku ume na-akpata yiri nke ahụ, mana ụzọ ọgwụgwọ, arụmọrụ na usoro nke ọrịa ndị dị iche iche na-akpata dị iche iche [4,5]. Ugbu a, ụzọ ndị bụ isi eji eme ihe n'ụlọ nyocha iji chọpụta nje ndị na-akpata iku ume a kpọtụrụ aha n'elu gụnyere: mwepụ nje, nchọpụta antigen na nchọpụta nucleic acid, wdg. Ngwa a na-achọpụta ma na-achọpụta ụfọdụ nje nucleic acid n'ime ndị mmadụ nwere ihe ịrịba ama na ihe mgbaàmà nke ọrịa iku ume, yana nchọpụta ụdị nke nje influenza na coronaviruses, ma na-ejikọta ya na nsonaazụ ụlọ nyocha ndị ọzọ iji nye aka na nchọpụta nke ọrịa nje iku ume. Nsonaazụ ọjọọ anaghị ewepụ ọrịa nje iku ume, ekwesighikwa iji ya dịka naanị ihe ndabere maka nchọpụta, ọgwụgwọ, ma ọ bụ mkpebi nchịkwa ndị ọzọ. Nsonaazụ dị mma enweghị ike iwepụ ọrịa nje bacteria ma ọ bụ ọrịa agwakọtara site na nje ndị ọzọ na-abụghị ihe ngosi nnwale. Ndị na-eme nnwale kwesịrị ịnata ọzụzụ ọkachamara na mmụba mkpụrụ ndụ ihe nketa ma ọ bụ nchọpụta bayọlọji, ma nwee asambodo ọrụ nnwale dị mkpa. Ụlọ nyocha ahụ kwesịrị inwe ebe nchekwa biosafety ezi uche dị na ya na usoro nchekwa.
Usoro Nkà na ụzụ
| Nchekwa | -18℃ |
| Ndụ nchekwa | Ọnwa itoolu |
| Ụdị ihe atụ | Swab akpịrị |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200Mpempe/mL |
| Nkọwapụta | Nsonaazụ nnwale mmeghachi omume gosiri na enweghị mmeghachi omume mmegharị ahụ n'etiti ngwa a na Cytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, ụdị Mycobacterium tuberculosis attenuated, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivaryus, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiraveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii, na asịd nucleic genomic nke mmadụ. |
| Ngwa Ndị Dị Mkpa | Sistemụ PCR Oge-N'ezie nke Biosystems 7500,Sistemụ PCR Ngwa-ngwa Ngwa Ngwa 7500 nke Ejiri Mee Ihe, QuantStudio®Sistemụ PCR 5 n'oge a, Sistemụ PCR SLAN-96P n'oge a na-ahụ anya (Hongshi Medical Technology Co., Ltd.), LightCycler®Sistemụ PCR oge 480, Sistemụ Nchọpụta PCR LineGene 9600 Plus nke oge a (FQD-96A, teknụzụ Hangzhou Bioer), MA-6000 Ngwa Okpomọkụ Oge A Na-ahụ Anya (Suzhou Molarray Co., Ltd.), Sistemụ PCR oge-oge BioRad CFX96, Sistemụ PCR BioRad CFX Opus 96 n'oge dị adị. |
Usoro Ọrụ
Ngwa Ngwuputa DNA/RNA nke Macro na Micro-Test Viral (HWTS-3017) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), na Ngwa Ngwuputa DNA/RNA nke Macro & Micro-Test Viral (HWTS-3017-8) (nke enwere ike iji ya na Eudemon)TM AIO800 (HWTS-EQ007)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Olu ihe nlele ewepụtara bụ 200μL, olu ihe mgbapụta akwadoro bụ 150μL.
