Ụdị 29 nke Ọrịa iku ume jikọtara Acid Nucleic
Aha ngwaahịa
HWTS-RT160-29 Ụdị Ọrịa iku ume jikọtara ngwa nchọpụta Nucleic Acid.
Epidemiology
Ọrịa iku ume bụ ọrịa a na-ahụkarị na ụmụ mmadụ, nke nwere ike ime na okike, afọ na mpaghara ọ bụla. Ọ bụ otu n'ime ihe na-ebute ọrịa na-anwụ anwụ na ọnụ ọgụgụ mmadụ n'ụwa nile[1]. Ọrịa ndị na-akpata iku ume na-agụnye novel coronavirus, Influenza A virus, Influenza B virus, Respiratory syncytial virus, Adenovirus, human metapneumovirus, rhinovirus, Parainfluenza virus type I/II/III, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniaeccus, wdg. Ihe mgbaàmà na ihe ịrịba ama nke ọrịa iku ume na-ebute bụ ihe yiri nke ahụ, ma ụzọ ọgwụgwọ, ịdị irè na ọrịa nke nje dị iche iche kpatara dị iche[4,5]. Ka ọ dị ugbu a, ụzọ ndị bụ isi a na-eji n'ụlọ nyocha iji chọpụta ọrịa nje iku ume ndị a kpọtụrụ aha n'elu gụnyere: iche iche nje, nchọpụta antigen na nchọpụta nucleic acid, wdg. Ngwa a na-achọpụta ma na-achọpụta kpọmkwem viral nucleic acids na ndị mmadụ nwere ihe ịrịba ama na mgbaàmà nke ọrịa iku ume, na ntinye nchọpụta nke nje virus influenza na coronaviruses, ma jikọta ya na nchọpụta ụlọ nyocha ndị ọzọ iji nye ọgwụgwọ ọrịa iku ume. Nsonaazụ na-adịghị mma anaghị ewepu ọrịa nje na-eku ume na e kwesịghị iji ya mee naanị ihe ndabere maka nyocha, ọgwụgwọ, ma ọ bụ mkpebi nlekọta ndị ọzọ. Nsonaazụ dị mma enweghị ike iwepụ nje na-efe efe ma ọ bụ ọrịa agwakọta nke nje ndị ọzọ na-abụghị ihe ngosi nnwale. Ndị na-ahụ maka nnwale kwesịrị ịnata ọzụzụ ọkachamara n'ihe gbasara mmụba mkpụrụ ndụ ihe nketa ma ọ bụ nchọpụta ihe ndụ ndụ, ma nwee ntozu nnwale dị mkpa. Ụlọ nyocha ahụ kwesịrị inwe akụrụngwa mgbochi biosafety ezi uche dị na ya na usoro nchebe.
Nka nka
| Nchekwa | -18 ℃ |
| Ndụ nchekwa | Ọnwa 9 |
| Ụdị ụdị | Akpịrị swab |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200 oyiri/μL |
| Nkọwapụta | Nsonaazụ nyocha nke cross-reactivity gosiri na enweghị mmeghachi omume n'etiti kit a na Cytomegalovirus, ụdị nje virus Herpes simplex 1, nje Varicella-zoster, nje Epstein-Barr, Pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophilubacterin a Morax, a na-akpọ Morax. ụkwara nta, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkriastrine, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, nucleictii acid gemnum. |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR, Ngwa biosystem 7500 ngwa ngwa PCR ngwa ngwa, QuantStudio®Sistemụ PCR 5 ezigbo oge, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LightCycler®480 ezigbo oge PCR usoro, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, teknụzụ Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Sistemụ PCR nke BioRad CFX96, BioRad CFX Opus 96 PCR ezigbo oge. |
Usoro ọrụ
Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (nke enwere ike iji ya na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), na Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017-8 nwere ike iji E).TM AIO800 (HWTS-EQ007)) nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ihe nlele nke ewepụtara bụ 200μL yana olu elution akwadoro bụ 150μL.
