Adenovirus Universal
Aha ngwaahịa
HWTS-RT017A Adenovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Adenovirus mmadụ (HAdV) bụ nke genus Mammalian adenovirus, nke bụ nje DNA nwere eriri abụọ na-enweghị envelopu.Adenoviruses nke achọtara ugbu a gụnyere 7 subgroups (AG) na ụdị 67, nke 55 serotypes bụ ọrịa na-efe efe nye mmadụ.N'ime ha, nke nwere ike ibute ọrịa akụkụ okuku ume bụ otu B (ụdị 3, 7, 11, 14, 16, 21, 50, 55), Group C (ụdị 1, 2, 5, 6, 57) na Group E. (Ụdị 4), nke nwere ike ibute ọrịa afọ ọsịsa nke eriri afọ bụ Group F (ụdị 40 na 41) [1-8].Ụdị dị iche iche nwere mgbaàmà ụlọ ọgwụ dị iche iche, mana ọ bụ ọrịa na-efe efe nke akụkụ okuku ume.Ọrịa iku ume na-ebute site na ọrịa iku ume nke ahụ mmadụ na-akpata 5% ~ 15% nke ọrịa iku ume zuru ụwa ọnụ, yana 5%-7% nke ọrịa iku ume ụmụaka.Adenovirus na-efe efe na mpaghara dịgasị iche iche ma nwee ike ibute ya n'afọ niile, karịsịa n'ebe ndị mmadụ juru, bụ nke na-ebute ọrịa mpaghara, karịsịa n'ụlọ akwụkwọ na ogige ndị agha.
Ọwa
| FAM | adenovirus zuru ụwa ọnụnucleic acid |
| ROX | Njikwa ime |
Nka nka
| Nchekwa | ≤-18℃ |
| Ndụ nchekwa | Ọnwa iri na abụọ |
| Ụdị ụdị | Nasopharyngeal swab,Akpịrị swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300 oyiri/ml |
| Nkọwapụta | a) Nyochaa nrụtụ aka na-adịghị mma nke ụlọ ọrụ ahazi site na ngwa ahụ, nsonaazụ ule na-emezu ihe achọrọ. b) Jiri ngwa a chọpụta na ọ dịghị cross-reactivity na ndị ọzọ iku ume nje (dị ka Influenza A nje, Influenza B nje, Respiratory syncytial virus, Parainfluenza virus, Rhinovirus, Human metapneumovirus, wdg) ma ọ bụ bacteria (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, wdg). |
| Ngwa ndị ọdabara | Ngwa Biosystem 7500 ezigbo oge PCR Sistemu Ngwa Biosystem 7500 Ngwa PCR ngwa ngwa ozugbo Sistemụ PCR ezigbo oge QuantStudio5 SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) Sistemụ PCR Real-Time LightCycler®480 Sistemụ nchọpụta PCR nke LineGene 9600 Pluss (FQD-96A, HangzhouTeknụzụ bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistemụ PCR nke BioRad CFX96, BioRad CFX Opus 96 Sistemụ PCR ezigbo oge |
Usoro ọrụ
(1) reagent mmịpụta akwadoro:Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Ekwesịrị ịme mmịpụta dịka ntuziaka ahụ si dị.Ihe nlele ewepụtara bụ ndị ọrịa'nasopharyngeal swab ma ọ bụ akpịrị swab sample anakọtara na saịtị.Tinye samples n'ime sample ntọhapụ reagent nke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex ka mix ọma, tinye n'ime ụlọ okpomọkụ maka 5 nkeji, wepụ na mgbe tụgharịa na mix ọma iji nweta DNA nke nke ọ bụla sample.
(2) reagent mmịpụta akwadoro:Macro & Micro-Nnwale Viral Ngwa DNA/RNA(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ekwesịrị ịrụ ọrụ ahụ n'ụzọ siri ike dabere na ntuziaka.Ihe nlele nke ewepụtara bụ 200μL, na nkeakwadoro elution oluis80μl.
(3) reagent mmịpụta akwadoro: Mwepụta Nucleic Acid ma ọ bụ Reagent Ọcha (YDP)315) site na Tiangen Biotech (Beijing) Co., Ltd., nkeEkwesịrị ịrụ ọrụ siri ike dabere na ntuziaka.Ihe nlele nke ewepụtara bụ 200μL, na nkeakwadoro elution oluis80μl.
